Mouse Anti-CYP21A2 Recombinant Antibody (EG887) (V2LY-0125-LY931)

Name: Mouse Anti-CYP21A2 Recombinant Antibody (EG887)
SKU: V2LY-0125-LY931
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

EG887

Application

Immunogen

Human recombinant protein fragment of human CYP21A2 produced in E.coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

BSA, Glycerol & PBS

Preservative

Sodium Azide

Concentration

1 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cytochrome P450 Family 21 Subfamily A Member 2

Entrez Gene ID

1589

UniProt ID

P08686

Function

A cytochrome P450 monooxygenase that plays a major role in adrenal steroidogenesis. Catalyzes the hydroxylation at C-21 of progesterone and 17alpha-hydroxyprogesterone to respectively form 11-deoxycorticosterone and 11-deoxycortisol, intermediate metabolites in the biosynthetic pathway of mineralocorticoids and glucocorticoids (PubMed:25855791, PubMed:10602386, PubMed:16984992, PubMed:22014889, PubMed:27721825).

Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:25855791).

Biological Process

Glucocorticoid biosynthetic process Source: GO_Central
Mineralocorticoid biosynthetic process Source: Reactome
Steroid biosynthetic process Source: UniProtKB
Steroid metabolic process Source: UniProtKB
Sterol metabolic process Source: Reactome

Cellular Location

Endoplasmic reticulum membrane; Microsome membrane

Involvement in disease

Adrenal hyperplasia 3 (AH3):
A form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: 'salt wasting' (SW, the most severe type), 'simple virilizing' (SV, less severely affected patients), with normal aldosterone biosynthesis, 'non-classic form' or late-onset (NC or LOAH) and 'cryptic' (asymptomatic).

Gangodkar, P., Khadilkar, V., Raghupathy, P., Kumar, R., Dayal, A. A., Dayal, D., ... & Khadilkar, A. (2021). Clinical application of a novel next generation sequencing assay for CYP21A2 gene in 310 cases of 21-hydroxylase congenital adrenal hyperplasia from India. Endocrine, 71(1), 189-198.

Prado, M. J., Singh, S., Ligabue-Braun, R., Meneghetti, B. V., Rispoli, T., Kopacek, C., ... & Pandey, A. V. (2021). Characterization of Mutations Causing CYP21A2 Deficiency in Brazilian and Portuguese Populations. International journal of molecular sciences, 23(1), 296.

Cohen, M., Pignatti, E., Dines, M., Mory, A., Ekhilevitch, N., Kolodny, R., ... & Tiosano, D. (2020). In silico structural and biochemical functional analysis of a novel CYP21A2 pathogenic variant. International journal of molecular sciences, 21(16), 5857.

Lin, B., Zhang, H., & Zheng, Q. (2020). How do mutations affect the structural characteristics and substrate binding of CYP21A2? An investigation by molecular dynamics simulations. Physical Chemistry Chemical Physics, 22(16), 8870-8877.

Malikova, J., Zingg, T., Fingerhut, R., Sluka, S., Grössl, M., Brixius-Anderko, S., ... & Flück, C. E. (2019). HIV drug efavirenz inhibits CYP21A2 activity with possible clinical implications. Hormone research in paediatrics, 91(4), 262-270.

Santos-Silva, R., Cardoso, R., Lopes, L., Fonseca, M., Espada, F., Sampaio, L., ... & Borges, T. (2019). CYP21A2 gene pathogenic variants: a multicenter study on genotype–phenotype correlation from a Portuguese pediatric cohort. Hormone Research in Paediatrics, 91(1), 33-45.

Concolino, P., & Costella, A. (2018). Congenital adrenal hyperplasia (CAH) due to 21-hydroxylase deficiency: a comprehensive focus on 233 pathogenic variants of CYP21A2 gene. Molecular diagnosis & therapy, 22(3), 261-280.

Simonetti, L., Bruque, C. D., Fernández, C. S., Benavides‐Mori, B., Delea, M., Kolomenski, J. E., ... & Dain, L. (2018). CYP21A2 mutation update: Comprehensive analysis of databases and published genetic variants. Human mutation, 39(1), 5-22.

Khajuria, R., Walia, R., Bhansali, A., & Prasad, R. (2017). The spectrum of CYP21A2 mutations in Congenital Adrenal Hyperplasia in an Indian cohort. Clinica Chimica Acta, 464, 189-194.

Concolino, P., Rizza, R., Costella, A., Carrozza, C., Zuppi, C., & Capoluongo, E. (2017). CYP21A2 intronic variants causing 21-hydroxylase deficiency. Metabolism, 71, 46-51.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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