Mouse Anti-EEF1B2 Recombinant Antibody (2G6) (CBMAB-A2462-LY)

The product is antibody recognizes EEF1B2. The antibody 2G6 immunoassay techniques such as: WB, ELISA.
See all EEF1B2 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

2G6

Antibody Isotype

IgG2a, κ

Application

WB, ELISA

Basic Information

Immunogen

EEF1B2 (AAH00211, 1 a.a. ~ 225 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Eukaryotic Translation Elongation Factor 1 Beta 2

Introduction

This gene encodes a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. Alternative splicing results in three transcript variants which differ only in the 5' UTR. [provided by RefSeq]

Entrez Gene ID

Human	1933
Mouse	55949
Rat	363241

UniProt ID

Human	P24534
Mouse	O70251
Rat	B5DEN5

Alternative Names

EEF1B; EEF1B1; EF1B

Research Area

EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP.

Biological Process

Response to ethanol Source: Ensembl
Translational elongation Source: GO_Central

Cellular Location

Cytosol; Cytoplasm; Eukaryotic translation elongation factor 1 complex

PTM

Phosphorylation affects the GDP/GTP exchange rate.

References

Gong, P., Liu, J., Jiao, X., Niu, Y., Wang, J., Wang, X., & Yang, Z. (2022). Novel biallelic loss of EEF1B2 function links to autosomal recessive intellectual disability. Human Mutation, 43(3), 299-304.

He, C., Guo, J., Tian, W., & Wong, C. C. (2021). Proteogenomics Integrating Novel Junction Peptide Identification Strategy Discovers Three Novel Protein Isoforms of Human NHSL1 and EEF1B2. Journal of Proteome Research, 20(12), 5294-5303.

Larcher, L., Buratti, J., Héron‐Longe, B., Benzacken, B., Pipiras, E., Keren, B., & Delahaye‐Duriez, A. (2020). New evidence that biallelic loss of function in EEF1B2 gene leads to intellectual disability. Clinical genetics, 97(4), 639-643.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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