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Mouse Anti-EMC1 Recombinant Antibody (CBFYE-0087) (CBMAB-E0182-FY)

This product is mouse antibody that recognizes EMC1. The antibody CBFYE-0087 can be used for immunoassay techniques such as: WB, IHC-P, IF.
See all EMC1 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Rat, Rabbit
Clone
CBFYE-0087
Antibody Isotype
IgG
Application
WB, IHC-P, IF

Basic Information

Immunogen
KLH conjugated peptide, human EMC1
Specificity
Human, Rat, Rabbit
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
1% BSA, 50% glycerol
Preservative
0.09% Sodium azide
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Er Membrane Protein Complex Subunit 1
Introduction
This gene encodes a single-pass type I transmembrane protein, which is a subunit of the endoplasmic reticulum membrane protein complex (EMC). Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Entrez Gene ID
Human23065
Rabbit100353694
Rat362643
UniProt ID
HumanQ8N766
RabbitG1SCY7
RatD4A994
Alternative Names
ER Membrane Protein Complex Subunit 1; KIAA0090; CAVIPMR
Research Area
Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:30415835, PubMed:29809151, PubMed:29242231, PubMed:32459176, PubMed:32439656).

Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed:30415835, PubMed:29809151, PubMed:29242231).

Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed:30415835, PubMed:29809151).

It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes (PubMed:29809151, PubMed:29242231).

By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors (PubMed:30415835).

By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable).
Biological Process
Protein insertion into ER membrane by stop-transfer membrane-anchor sequence Source: UniProtKB
Tail-anchored membrane protein insertion into ER membrane Source: UniProtKB
Cellular Location
Endoplasmic reticulum membrane
Involvement in disease
Cerebellar atrophy, visual impairment, and psychomotor retardation (CAVIPMR):
An autosomal recessive, neurodegenerative disorder characterized by developmental delay, intellectual disability, hypotonia, scoliosis, cerebellar atrophy, and variable dysmorphic features.
Topology
Lumenal: 23-962
Helical: 963-983
Cytoplasmic: 984-993

Iyer, A., Niemann, M., Serricchio, M., Dewar, C. E., Oeljeklaus, S., Farine, L., ... & Bütikofer, P. (2022). The endoplasmic reticulum membrane protein complex localizes to the mitochondrial-endoplasmic reticulum interface and its subunits modulate phospholipid biosynthesis in Trypanosoma brucei. PLoS pathogens, 18(5), e1009717.

Kawata, K., Baba, A., Shiota, M., Wanibuchi, H., & Baba, Y. (2021). ER membrane protein complex 1 interacts with STIM1 and regulates store-operated Ca2+ entry. The Journal of Biochemistry, 170(4), 483-488.

Pleiner, T., Tomaleri, G. P., Januszyk, K., Inglis, A. J., Hazu, M., & Voorhees, R. M. (2020). Structural basis for membrane insertion by the human ER membrane protein complex. Science, 369(6502), 433-436.

Marquez, J., Criscione, J., Charney, R. M., Prasad, M. S., Hwang, W. Y., Mis, E. K., ... & Khokha, M. K. (2020). Disrupted ER membrane protein complex–mediated topogenesis drives congenital neural crest defects. The Journal of clinical investigation, 130(2), 813-826.

Bai, L., You, Q., Feng, X., Kovach, A., & Li, H. (2020). Structure of the ER membrane complex, a transmembrane-domain insertase. Nature, 584(7821), 475-478.

Lin, D. L., Inoue, T., Chen, Y. J., Chang, A., Tsai, B., & Tai, A. W. (2019). The ER membrane protein complex promotes biogenesis of dengue and Zika virus non-structural multi-pass transmembrane proteins to support infection. Cell reports, 27(6), 1666-1674.

Shurtleff, M. J., Itzhak, D. N., Hussmann, J. A., Oakdale, N. T. S., Costa, E. A., Jonikas, M., ... & Weissman, J. S. (2018). The ER membrane protein complex interacts cotranslationally to enable biogenesis of multipass membrane proteins. Elife, 7, e37018.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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