Rabbit Anti-EMC10 Recombinant Antibody (CBWJC-3603) (CBMAB-C4844WJ)

This product is a Rabbit antibody that recognizes EMC10. This antibody CBWJC-3603 can be used for immunoassay techniques such as: WB, FC, ICC.
See all EMC10 antibodies

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human

Clone

CBWJC-3603

Antibody Isotype

IgG

Application

WB, FC, ICC

Basic Information

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Concentration

1.162 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

ER Membrane Protein Complex Subunit 10

Introduction

EMC10 (ER Membrane Protein Complex Subunit 10) is a Protein Coding gene.

Entrez Gene ID

284361

UniProt ID

Q5UCC4

Alternative Names

ER Membrane Protein Complex Subunit 10; Hematopoietic Signal Peptide-Containing Membrane Domain-Containing 1; Hematopoietic Signal Peptide-Containing Secreted 1; C19orf63; HSM1; Hematopoietic Signal Peptide-Containing Membrane Domain-Containing Protein 1;

Research Area

Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:30415835, PubMed:29809151, PubMed:29242231, PubMed:32459176, PubMed:32439656).

Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed:30415835, PubMed:29809151, PubMed:29242231).

Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed:30415835, PubMed:29809151).

It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes (PubMed:29809151, PubMed:29242231).

By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors (PubMed:30415835).

By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable). Promotes angiogenesis and tissue repair in the heart after myocardial infarction. Stimulates cardiac endothelial cell migration and outgrowth via the activation of p38 MAPK, PAK and MAPK2 signaling pathways (PubMed:28931551).

Biological Process

Angiogenesis Source: UniProtKB-KW
Positive regulation of angiogenesis Source: UniProtKB
Positive regulation of endothelial cell migration Source: UniProtKB
Positive regulation of endothelial cell proliferation Source: UniProtKB
Positive regulation of p38MAPK cascade Source: UniProtKB
Protein insertion into ER membrane by stop-transfer membrane-anchor sequence Source: UniProtKB
Tail-anchored membrane protein insertion into ER membrane Source: UniProtKB

Cellular Location

Isoform 1: Endoplasmic reticulum membrane
Isoform 2: Secreted

Topology

Lumenal: 26-221
Helical: 222-242
Cytoplasmic: 243-262

PTM

Glycosylated.

References

Liu, L., Mao, S., Chen, K., Dai, J., Jin, S., Chen, L., ... & Wang, X. (2022). Membrane-Bound EMC10 Is Required for Sperm Motility via Maintaining the Homeostasis of Cytoplasm Sodium in Sperm. International journal of molecular sciences, 23(17), 10069.

Kaiyrzhanov, R., Rocca, C., Suri, M., Gulieva, S., Zaki, M. S., Henig, N. Z., ... & Maroofian, R. (2022). Biallelic loss of EMC10 leads to mild to severe intellectual disability. Annals of Clinical and Translational Neurology, 9(7), 1080-1089.

Shao, D. D., Straussberg, R., Ahmed, H., Khan, A., Tian, S., Hill, R. S., ... & Walsh, C. A. (2021). A recurrent, homozygous EMC10 frameshift variant is associated with a syndrome of developmental delay with variable seizures and dysmorphic features. Genetics in Medicine, 23(6), 1158-1162.

Umair, M., Ballow, M., Asiri, A., Alyafee, Y., Al Tuwaijri, A., Alhamoudi, K. M., ... & Alfadhel, M. (2020). EMC10 homozygous variant identified in a family with global developmental delay, mild intellectual disability, and speech delay. Clinical Genetics, 98(6), 555-561.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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