Rat Anti-EXTL2 (AA 43-330) Recombinant Antibody (CBFYE-1421) (CBMAB-E2045-FY)

This product is rat antibody that recognizes EXTL2. The antibody CBFYE-1421 can be used for immunoassay techniques such as: WB.
See all EXTL2 antibodies

Datasheet

Summary

Host Animal

Rat

Specificity

Mouse

Clone

CBFYE-1421

Antibody Isotype

IgG2a

Application

Basic Information

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Exostosin-like 2/EXTL2, Asn43-Met330

Specificity

Mouse

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, trehalose

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 43-330

Target

Full Name

Exostosin Like Glycosyltransferase 2

Introduction

EXTL2 (Exostosin Like Glycosyltransferase 2) is a Protein Coding gene. Diseases associated with EXTL2 include Hereditary Multiple Osteochondromas. Among its related pathways are heparan sulfate biosynthesis and Metabolism. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring hexosyl groups and alpha-1,4-N-acetylgalactosaminyltransferase activity. An important paralog of this gene is EXTL3.

Entrez Gene ID

58193

UniProt ID

Q9ES89

Alternative Names

AW146439; 3000001D04Rik

Research Area

Glycosyltransferase required for the biosynthesis of heparan-sulfate and responsible for the alternating addition of beta-1-4-linked glucuronic acid (GlcA) and alpha-1-4-linked N-acetylglucosamine (GlcNAc) units to nascent heparan sulfate chains.

Biological Process

Heparan sulfate proteoglycan biosynthetic process Source: UniProtKB-UniPathway
N-acetylglucosamine metabolic process Source: UniProtKB
Protein glycosylation Source: InterPro
UDP-N-acetylgalactosamine metabolic process Source: UniProtKB

Cellular Location

Endoplasmic reticulum membrane
Processed exostosin-like 2: Secreted. A soluble form is found in the serum.

Topology

Cytoplasmic: 1-22
Helical: 23-43
Lumenal: 44-330

PTM

The soluble form derives from the membrane form by proteolytic processing.

References

Marques, C., Poças, J., Gomes, C., Faria-Ramos, I., Reis, C. A., Vivès, R. R., & Magalhães, A. (2022). Glycosyltransferases EXTL2 and EXTL3 cellular balance dictates Heparan Sulfate biosynthesis and shapes gastric cancer cell motility and invasion. Journal of Biological Chemistry, 102546.

Lei, J., Deng, H., Ran, Y., Lv, Y., Amhare, A. F., Wang, L., ... & Lammi, M. J. (2021). Altered expression of aggrecan, FAM20B, B3GALT6, and EXTL2 in patients with osteoarthritis and Kashin-beck disease. Cartilage, 13(1_suppl), 818S-828S.

Pu, A., Mishra, M. K., Dong, Y., Ghorbanigazar, S., Stephenson, E. L., Rawji, K. S., ... & Yong, V. W. (2020). The glycosyltransferase EXTL2 promotes proteoglycan deposition and injurious neuroinflammation following demyelination. Journal of Neuroinflammation, 17(1), 1-21.

Nadanaka, S., Hashiguchi, T., & Kitagawa, H. (2020). Aberrant glycosaminoglycan biosynthesis by tumor suppressor EXTL2 deficiency promotes liver inflammation and tumorigenesis through Toll‐like 4 receptor signaling. The FASEB Journal, 34(6), 8385-8401.

Pu, Y. (2019). The glycosyltransferase EXTL2 and its regulation of remyelination and neuroinflammation (Master's thesis, Cumming School of Medicine).

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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