Mouse Anti-FADS3 Recombinant Antibody (CBXF-2823) (CBMAB-F3533-CQ)

This product is a mouse antibody that recognizes FADS3. The antibody CBXF-2823 can be used for immunoassay techniques such as: ELISA, WB.
See all FADS3 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBXF-2823

Antibody Isotype

IgG2b, κ

Application

ELISA, WB

Basic Information

Immunogen

Partial recombinant corresponding to aa16-113 from human FADS3 (NP_068373) with GST tag

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Fatty Acid Desaturase 3

Introduction

The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization.

Entrez Gene ID

3995

UniProt ID

Q9Y5Q0

Alternative Names

Fatty Acid Desaturase 3; Cytochrome B5-Related Protein; Delta-9-Desaturase; CYB5RP; Linoleoyl-CoA Desaturase (Delta-9-Desaturase)-Like 3; Delta-9 Fatty Acid Desaturase; EC 1.14.19.-; EC 1.14.19; LLCDL3;

Research Area

Mammals have different sphingoid bases that differ in their length and/or pattern of desaturation and hydroxyl groups. The predominant sphingoid base in mammalian ceramides is sphing-4-enine (sphingosine or SPH) which has a trans desaturation at carbon 4. FADS3 is a ceramide desaturase that introduces a cis double bond between carbon 14 and carbon 15 of the SPH-containing ceramides, producing sphinga-4,14-dienine-containing ceramides (SPD ceramides). SPD ceramides occur widely in mammalian tissues and cells. Due to their unusual structure containing a cis double bond, SPD ceramides may have an opposite, negative role in lipid microdomain formation relative to conventional ceramides (PubMed:31916624).

FADS3 also acts as a methyl-end fatty acyl coenzyme A (CoA) desaturase that introduces a cis double bond between the preexisting double bond and the terminal methyl group of the fatty acyl chain. Desaturates (11E)-octadecenoate (trans-vaccenoate, the predominant trans fatty acid in human milk) at carbon 13 to generate (11E,13Z)-octadecadienoate (also known as conjugated linoleic acid 11E,13Z-CLA).

Biological Process

Sphingolipid metabolic process Source: UniProtKB-UniPathway
Unsaturated fatty acid biosynthetic process Source: UniProtKB

Cellular Location

Endoplasmic reticulum membrane

Topology

Cytoplasmic: 1-133
Helical: 134-154
Lumenal: 155-159
Helical: 160-180
Cytoplasmic: 181-263
Helical: 264-284
Lumenal: 285-306
Helical: 307-327
Cytoplasmic: 328-445

References

Karsai, G., Lone, M., Kutalik, Z., Brenna, J. T., Li, H., Pan, D., ... & Hornemann, T. (2020). FADS3 is a Δ14Z sphingoid base desaturase that contributes to gender differences in the human plasma sphingolipidome. Journal of Biological Chemistry, 295(7), 1889-1897.

Jojima, K., Edagawa, M., Sawai, M., Ohno, Y., & Kihara, A. (2020). Biosynthesis of the anti‐lipid‐microdomain sphingoid base 4, 14‐sphingadiene by the ceramide desaturase FADS3. The FASEB Journal, 34(2), 3318-3335.

Rioux, V., & Legrand, P. (2019). Fatty Acid Desaturase 3 (FADS3) Is a Specific∆ 13-Desaturase of Ruminant trans-Vaccenic Acid. Lifestyle Genomics, 12(1-6), 18-24.

Yang, H. T., Wang, R. Y., Huang, S. Y., Huang, C. L., & Su, K. P. (2018). Genetic polymorphisms of FADS1, FADS2, and FADS3 and fatty acid profiles in subjects received methadone maintenance therapy. Prostaglandins, Leukotrienes and Essential Fatty Acids, 136, 117-121.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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