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Mouse Anti-FBXL5 Recombinant Antibody (CBXF-1452) (CBMAB-F1111-CQ)

This product is a mouse antibody that recognizes FBXL5. The antibody CBXF-1452 can be used for immunoassay techniques such as: WB, ELISA.
See all FBXL5 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBXF-1452
Antibody Isotype
IgG2a
Application
WB, ELISA

Basic Information

Immunogen
Recombinant protein
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
F-box and leucine-rich repeat protein 5
Introduction
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains several tandem leucine-rich repeats. Alternatively spliced transcript variants have been described for this locus.
Entrez Gene ID
26234
UniProt ID
Q9UKA1
Alternative Names
F-Box And Leucine Rich Repeat Protein 5; F-Box Protein FBL4/FBL5; P45SKP2-Like Protein; FBL4; FBL5; FLR1; F-Box And Leucine-Rich Repeat Protein 5; F-Box/LRR-Repeat Protein 5;
Research Area
Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2. Upon high iron and oxygen level, it specifically recognizes and binds IREB2/IRP2, promoting its ubiquitination and degradation by the proteasome. Promotes ubiquitination and subsequent degradation of DCTN1/p150-glued.
Biological Process
Iron ion homeostasis Source: UniProtKB
Positive regulation of cellular protein catabolic process Source: Ensembl
Protein ubiquitination Source: UniProtKB
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
Cellular Location
Perinuclear region
PTM
Polybiquitinated upon iron and oxygen depletion, leading to its degradation by the proteasome. Ubiquitination is regulated by the hemerythrin-like region that acts as an oxygen and iron sensor.

Li, D., You, J., Mao, C., Zhou, E., Han, Z., Zhang, J., ... & Wang, C. (2022). Circular RNA Fbxl5 Regulates Cardiomyocyte Apoptosis During Ischemia Reperfusion Injury via Sponging microRNA-146a. Journal of Inflammation Research, 15, 2539.

Terzi, E. M., Sviderskiy, V. O., Alvarez, S. W., Whiten, G. C., & Possemato, R. (2021). Iron-sulfur cluster deficiency can be sensed by IRP2 and regulates iron homeostasis and sensitivity to ferroptosis independent of IRP1 and FBXL5. Science Advances, 7(22), eabg4302.

Park, C. K., Heo, J., Ham, W. S., Choi, Y. D., Shin, S. J., & Cho, N. H. (2021). Ferroportin and FBXL5 as Prognostic Markers in Advanced Stage Clear Cell Renal Cell Carcinoma. Cancer Research and Treatment: Official Journal of Korean Cancer Association, 53(4), 1174-1183.

Wang, H., Shi, H., Rajan, M., Canarie, E. R., Hong, S., Simoneschi, D., ... & Zheng, N. (2020). FBXL5 regulates IRP2 stability in iron homeostasis via an oxygen-responsive [2Fe2S] cluster. Molecular cell, 78(1), 31-41.

Rouault, T. A., & Maio, N. (2020). How oxidation of a unique iron-sulfur cluster in FBXL5 regulates IRP2 levels and promotes regulation of iron metabolism proteins. Molecular cell, 78(1), 1-3.

Mayank, A. K., Pandey, V., Vashisht, A. A., Barshop, W. D., Rayatpisheh, S., Sharma, T., ... & Wohlschlegel, J. A. (2019). An oxygen-dependent interaction between FBXL5 and the CIA-targeting complex regulates iron homeostasis. Molecular cell, 75(2), 382-393.

Jiao, Q., Du, X., Wei, J., Li, Y., & Jiang, H. (2019). Oxidative stress regulated iron regulatory protein IRP2 through FBXL5-mediated ubiquitination-proteasome way in SH-SY5Y cells. Frontiers in Neuroscience, 13, 20.

Muto, Y., Moroishi, T., Ichihara, K., Nishiyama, M., Shimizu, H., Eguchi, H., ... & Nakayama, K. I. (2019). Disruption of FBXL5-mediated cellular iron homeostasis promotes liver carcinogenesis. Journal of Experimental Medicine, 216(4), 950-965.

Yin, X., Yuan, M., Duan, Y., Zhang, S., Wu, Y., & Wang, J. (2019). Association between Fbxl5 gene polymorphisms and partial economic traits in Jinghai Yellow chickens. Archives Animal Breeding, 62(1), 91-97.

Das, N. K., Sandhya, S., Kumar, R., Singh, A. K., Bal, S. K., Kumari, S., & Mukhopadhyay, C. K. (2018). Leishmania donovani inhibits ferroportin translation by modulating FBXL5‐IRP2 axis for its growth within host macrophages. Cellular Microbiology, 20(7), e12834.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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