Mouse Anti-H2AFZ Recombinant Antibody (EG1473) (CBMAB-EN1760-LY)

The product is antibody recognizes H2AFZ. The antibody EG1473 immunoassay techniques such as: ELISA, WB.
See all H2AFZ antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

EG1473

Antibody Isotype

IgG

Application

ELISA, WB

Basic Information

Immunogen

A synthetic peptide of human H2AFZ.

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

H2A Histone Family Member Z

Introduction

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008]

Entrez Gene ID

2997

UniProt ID

P13807

Alternative Names

H2A Histone Family Member Z; H2A/Z; H2AZ; H2A Histone Family, Member Z; Histone H2A.Z; H2AZ Histone; H2A.Z-1; H2A.Z;

Function

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.

Biological Process

Cellular response to estradiol stimulus Source: UniProtKB
Heterochromatin assembly Source: GO_Central
Positive regulation of transcription by RNA polymerase II Source: UniProtKB

Cellular Location

Nucleus; Chromosome

PTM

Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
Acetylated on Lys-5, Lys-8, Lys-12 and Lys-14 by KAT2A; KAT2A is recruited by the XPC complex in absence of DNA damage (PubMed:31527837). Acetylated on Lys-5, Lys-8 and Lys-12 during interphase; acetylation disappears at mitosis (By similarity). Acetylation by the NuA4 histone acetyltransferase complex is required for hematopoietic stem cell maintenance (By similarity).
Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
Not phosphorylated.
Lactylated in macrophages by EP300/P300 by using lactoyl-CoA directly derived from endogenous or exogenous lactate, leading to stimulates gene transcription.

References

Ye, L., Fan, T., Qin, Y., Qiu, C., Li, L., Dai, M., ... & Jiang, Y. (2023). MicroRNA-455-3p accelerate malignant progression of tumor by targeting H2AFZ in colorectal cancer. Cell Cycle, 22(7), 777-795.

Lubin, E., Bryant, L., Aicher, J., Li, D., & Bhoj, E. (2022). Analysis of histone variant constraint and tissue expression suggests five potential novel human disease genes: H2AFY2, H2AFZ, H2AFY, H2AFV, H1F0. Human Genetics, 141(8), 1409-1421.

Li, Z., Hu, M., Qiu, J., Feng, J., Zhang, R., Wu, H., ... & Ren, J. (2022). H2A Histone Family Member Z (H2AFZ) Serves as a Prognostic Biomarker in Lung Adenocarcinoma: Bioinformatic Analysis and Experimental Validation. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research, 28, e933447-1.

Dong, M., Chen, J., Deng, Y., Zhang, D., Dong, L., & Sun, D. (2021). H2AFZ is a prognostic biomarker correlated to TP53 mutation and immune infiltration in hepatocellular carcinoma. Frontiers in Oncology, 11, 701736.

Bongiovanni, L., Andriessen, A., Silvestri, S., Porcellato, I., Brachelente, C., & De Bruin, A. (2021). H2AFZ: a novel prognostic marker in canine melanoma and a predictive marker for resistance to CDK4/6 inhibitor treatment. Frontiers in Veterinary Science, 8, 705359.

Zhao, L., Ma, C., Song, H., Feng, Y., Li, Y., Xia, X., ... & Li, F. (2019). H2AFZ, RNF4 and NR4A1 loci are associated with boar semen quality by population association studies. Animal Biotechnology, 30(4), 311-316.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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