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Mouse Anti-HIRA Recombinant Antibody (CBFYH-1138) (CBMAB-H2094-FY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYH-1138
Antibody Isotype
IgG2a
Application
WB

Basic Information

Immunogen
Recombinant human HIR Histone Cell Cycle Regulation Defective Homolog A
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Histone Cell Cycle Regulator
Introduction
This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development.
Entrez Gene ID
7290
UniProt ID
P54198
Alternative Names
Histone Cell Cycle Regulator; TUP1-Like Enhancer Of Split Protein 1; DiGeorge Critical Region Gene 1; TUPLE1; DGCR1; HIR (Histone Cell Cycle Regulation Defective) Homolog A (S. Cerevisiae)
Function
Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
Biological Process
Anatomical structure morphogenesis Source: ProtInc
DNA replication-independent chromatin assembly Source: UniProtKB
Gastrulation Source: Ensembl
Muscle cell differentiation Source: Ensembl
Osteoblast differentiation Source: Ensembl
Regulation of transcription by RNA polymerase II Source: ProtInc
Transcription, DNA-templated Source: InterPro
Cellular Location
Nucleus; PML body. Primarily, though not exclusively, localized to the nucleus. Localizes to PML bodies immediately prior to onset of senescence.
PTM
Sumoylated.
Phosphorylated by CDK2/CCNA1 and CDK2/CCNE1 on Thr-555 in vitro. Also phosphorylated on Thr-555 and Ser-687 in vivo.
More Infomation

Zhang, M., Zhao, X., Feng, X., Hu, X., Zhao, X., Lu, W., & Lu, X. (2022). Histone chaperone HIRA complex regulates retrotransposons in embryonic stem cells. Stem Cell Research & Therapy, 13(1), 137.

Valcarcel-Jimenez, L., Rogerson, C., Yong, C., Schmidt, C., Yang, M., Cremades-Rodelgo, M., ... & Frezza, C. (2022). HIRA loss transforms FH-deficient cells. Science advances, 8(42), eabq8297.

Layat, E., Bourcy, M., Cotterell, S., Zdzieszyńska, J., Desset, S., Duc, C., ... & Probst, A. V. (2021). The Histone chaperone HIRA is a positive regulator of seed germination. International Journal of Molecular Sciences, 22(8), 4031.

Bouvier, D., Ferrand, J., Chevallier, O., Paulsen, M. T., Ljungman, M., & Polo, S. E. (2021). Dissecting regulatory pathways for transcription recovery following DNA damage reveals a non-canonical function of the histone chaperone HIRA. Nature Communications, 12(1), 3835.

Zhang, Y., Jiang, C., Trudeau, S. J., Narita, Y., Zhao, B., Teng, M., ... & Gewurz, B. E. (2020). Histone loaders CAF1 and HIRA restrict Epstein-Barr virus B-cell lytic reactivation. MBio, 11(5), 10-1128.

McFarlane, S., Orr, A., Roberts, A. P., Conn, K. L., Iliev, V., Loney, C., ... & Boutell, C. (2019). The histone chaperone HIRA promotes the induction of host innate immune defences in response to HSV-1 infection. PLoS Pathogens, 15(3), e1007667.

Majumder, A., Dharan, A. T., Baral, I., Varghese, P. C., Mukherjee, A., Subhadradevi, L., ... & Dutta, D. (2019). Histone chaperone HIRA dictate proliferation vs differentiation of chronic myeloid leukemia cells. FASEB bioAdvances, 1(9), 525.

Shindo, T., Doi, S., Nakashima, A., Sasaki, K., Arihiro, K., & Masaki, T. (2018). TGF-β1 promotes expression of fibrosis-related genes through the induction of histone variant H3. 3 and histone chaperone HIRA. Scientific reports, 8(1), 14060.

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For research use only. Not intended for any clinical use.

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