Mouse Anti-NAP1L4 Recombinant Antibody (CBWJN-0644) (CBMAB-N1148-WJ)

Name: Mouse Anti-NAP1L4 Recombinant Antibody (CBWJN-0644)
SKU: CBMAB-N1148-WJ
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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBWJN-0644

Antibody Isotype

IgG1, κ

Application

ELISA, BL, WB

Basic Information

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Nucleosome Assembly Protein 1 Like 4

Introduction

This gene encodes a member of the nucleosome assembly protein (NAP) family which can interact with both core and linker histones. It can shuttle between the cytoplasm and nucleus, suggesting a role as a histone chaperone. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. [provided by RefSeq, Jul 2008]

Entrez Gene ID

4676

UniProt ID

Q99733

Alternative Names

Nucleosome Assembly Protein 1 Like 4; Nucleosome Assembly Protein 1-Like 4; Nucleosome Assembly Protein 2; NAP-2; NAP2; Nucleosome Assembly Protein 1-Like 4b; NAP1L4b; NAP2L; HNAP2;

Function

Acts as histone chaperone in nucleosome assembly.

Biological Process

Nucleosome assembly Source: UniProtKB

Cellular Location

Nucleus
Cytoplasm 2 Publications
Note: Present in the cytoplasm and excluded from the nucleus during G0/G1 phase, then relocates to the nucleus by the time cells are in S phase (PubMed:9325046). Phosphorylated form localizes in the cytoplasm during the G0/G1 transition, whereas dephosphorylation leads to relocalization into the nucleus at the G1/S-boundary (PubMed:10764593).

PTM

Phosphorylated at the G0/G1 boundary but it is not phosphorylated in S-phase. Phosphorylated protein remains in the cytoplasm in a complex with histones during the G0/G1 transition, whereas dephosphorylation triggers its transport into the nucleus at the G1/S-boundary.
Polyglutamylated by TTLL4, a modification that occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Some residues may also be monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human (By similarity).

More Infomation

References

Dominguez, F., Shiliaev, N., Lukash, T., Agback, P., Palchevska, O., Gould, J. R., ... & Frolov, I. (2021). NAP1L1 and NAP1L4 binding to hypervariable domain of chikungunya virus nsP3 protein is bivalent and requires phosphorylation. Journal of Virology, 95(16), 10-1128.

Wang, S., Ren, X., Li, J., Lin, C., Zhou, J., Zhou, J., & Gu, J. (2020). NAP1L4 inhibits porcine circovirus type 2 replication via IFN-β signaling pathway. Veterinary Microbiology, 246, 108692.

Cheng, Z., Luo, Y., Zhang, Y., Wang, Y., Chen, Y., Xu, Y., ... & Zhang, G. (2020). A novel NAP1L4/NUTM1 fusion arising from translocation t (11; 15)(p15; q12) in a myeloid neoplasm with eosinophilia and rearrangement of PDGFRA highlights an unusual clinical feature and therapeutic reaction. Annals of Hematology, 99, 1561-1564.

Tanaka, T., Hozumi, Y., Martelli, A. M., Iino, M., & Goto, K. (2019). Nucleosome assembly proteins NAP1L1 and NAP1L4 modulate p53 acetylation to regulate cell fate. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1866(12), 118560.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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