Mouse Anti-SKB1 Recombinant Antibody (4F2) (CBMAB-A8153-LY)

Mouse

Human

4F2

IgG2b, κ

ELISA

SKB1 (AAH25979.1, 1 a.a. ~ 637 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Human

IgG2b, κ

Monoclonal

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Liquid

> 95% Purity determined by SDS-PAGE.

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Protein Arginine Methyltransferase 5

HRMT1L5; IBP72; JBP1; SKB1; SKB1Hs

Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA (PubMed:10531356, PubMed:11152681, PubMed:11747828, PubMed:12411503, PubMed:15737618, PubMed:17709427, PubMed:20159986, PubMed:20810653, PubMed:21258366, PubMed:21917714, PubMed:22269951, PubMed:21081503).
Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles (PubMed:12411503, PubMed:11747828, PubMed:17709427).
Methylates SUPT5H and may regulate its transcriptional elongation properties (PubMed:12718890).
Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. Methylates histone H2A and H4 'Arg-3' during germ cell development (By similarity).
Methylates histone H3 'Arg-8', which may repress transcription (By similarity).
Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage (By similarity).
Methylates RPS10. Attenuates EGF signaling through the MAPK1/MAPK3 pathway acting at 2 levels. First, monomethylates EGFR; this enhances EGFR 'Tyr-1197' phosphorylation and PTPN6 recruitment, eventually leading to reduced SOS1 phosphorylation (PubMed:21917714, PubMed:21258366).
Second, methylates RAF1 and probably BRAF, hence destabilizing these 2 signaling proteins and reducing their catalytic activity (PubMed:21917714).
Required for induction of E-selectin and VCAM-1, on the endothelial cells surface at sites of inflammation. Methylates HOXA9 (PubMed:22269951).
Methylates and regulates SRGAP2 which is involved in cell migration and differentiation (PubMed:20810653).
Acts as a transcriptional corepressor in CRY1-mediated repression of the core circadian component PER1 by regulating the H4R3 dimethylation at the PER1 promoter (By similarity).
Methylates GM130/GOLGA2, regulating Golgi ribbon formation (PubMed:20421892).
Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (PubMed:25284789).
Symmetrically methylates POLR2A, a modification that allows the recruitment to POLR2A of proteins including SMN1/SMN2 and SETX. This is required for resolving RNA-DNA hybrids created by RNA polymerase II, that form R-loop in transcription terminal regions, an important step in proper transcription termination (PubMed:26700805).
Along with LYAR, binds the promoter of gamma-globin HBG1/HBG2 and represses its expression (PubMed:25092918).
Symmetrically methylates NCL (PubMed:21081503).
Methylates p53/TP53; methylation might possibly affect p53/TP53 target gene specificity (PubMed:19011621).
Involved in spliceosome maturation and mRNA splicing in prophase I spermatocytes through the catalysis of the symmetrical arginine dimethylation of SNRPB (small nuclear ribonucleoprotein-associated protein) and the interaction with tudor domain-containing protein TDRD6 (By similarity).

Chromatin organizationIEA:UniProtKB-KW
Circadian regulation of gene expressionISS:UniProtKB
DNA-templated transcription, terminationManual Assertion Based On ExperimentIMP:UniProtKB
Endothelial cell activationManual Assertion Based On ExperimentIMP:UniProtKB
Golgi ribbon formationManual Assertion Based On ExperimentIMP:UniProtKB
Histone arginine methylationManual Assertion Based On ExperimentIBA:GO_Central
Histone H4-R3 methylationManual Assertion Based On ExperimentIDA:WormBase
Liver regenerationIEA:Ensembl
Negative regulation of cell differentiationIEA:Ensembl
Peptidyl-arginine methylationManual Assertion Based On ExperimentIMP:UniProtKB
Peptidyl-arginine N-methylationManual Assertion Based On ExperimentIDA:WormBase
Positive regulation of adenylate cyclase-inhibiting dopamine receptor signaling pathwayManual Assertion Based On ExperimentIGI:WormBase
Positive regulation of mRNA splicing, via spliceosome1 PublicationIC:ComplexPortal
Positive regulation of oligodendrocyte differentiationIEA:Ensembl
Regulation of DNA methylationIEA:Ensembl
Regulation of ERK1 and ERK2 cascadeIEA:Ensembl
Regulation of mitotic nuclear divisionManual Assertion Based On ExperimentTAS:ProtInc
Regulation of signal transduction by p53 class mediatorTAS:Reactome
Regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIBA:GO_Central
Spliceosomal snRNP assemblyManual Assertion Based On ExperimentIMP:UniProtKB

Cytoplasm
Nucleus
Chromosome
Golgi apparatus
Localizes to promoter regions of target genes on chromosomes.