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Human Atherosclerosis Antibody Array (Membrane, 20 Targets) (Y0007) (AbAr-0007-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Human Atherosclerosis Antibody Array detects 20 Human Atherosclerotic Factors. It is suitable for all liquid sample types.
Specifications
Target Array
Atherosclerosis Array
Target
Eotaxin-1 (CCL11), IFN-gamma, MCP-1 (CCL2), TNF alpha, GCSF, IL-1 alpha (IL-1 F1), M-CSF, TNF beta (TNFSF1B), GDNF, IL-1 beta (IL-1 F2), TGF beta 1, MIP-3 alpha (CCL20), GM-CSF, IL-1 R1, TGF beta 2, PDGF-BB, ICAM-1 (CD54), IL-1 R2, TGF beta 3, RANTES (CCL5)
Species Reactivity
Human
Sample Type
Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates
Applications
Application
Multiplex Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Membrane
Detection Assay
Sandwich-based
Protocol Outline
Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.
Background
Introduction
Atherosclerosis is a disease in which plaque builds up inside your arteries. Arteries are blood vessels that carry oxygen-rich blood to your heart and other parts of your body. Plaque is made up of fat, cholesterol, calcium, and other substances found in the blood. Over time, plaque hardens and narrows your arteries.
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For Research Use Only. Not For Clinical Use.