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Human Kidney Biomarker Antibody Array (Membrane, 38 Targets) (Y0252) (AbAr-0252-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Human Kidney Biomarker Antibody Array can be used to simultaneously detect the relative changes of 38 proteins in a single sample.
Target Array
Kidney Biomarker Array
Adiponectin, Fetuin A, Renin, Aminopeptidase N, GRO-alpha/CXCL1, Resistin, Angiotensinogen/Serpin A8, IL-1ra, SCF, Annexin V, IL-6, Serpin A3, beta2-Microglobulin, IL-10, TNF-alpha, Clusterin, TIM-1/KIM-1/HAVCR, TNF RI, CXCL16, Lipocalin-2/NGAL, Trefoil Factor 3, Cyr61/CCN, MCP-1/CCL2, Thrombospondin-1, Cystatin C, MMP-9, TWEAK, DPPIV/CD26, Neprilysin, uPA, EGF, PSA/KLK-3, VCAM-1, EGF R/ErbB1, RAGE, VEGF-A, FABP1/L-FABP, RBP-4
Species Reactivity
Multiplex Protein Detection
Solid Support
Detection Assay
Protocol Outline
Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.
Legacy kidney biomarkers include serum creatinine (sCr), blood urea nitrogen (BUN), urinary albumin/protein and volume excretion. However, sCr or BUN cannot distinguish injury from hemodynamic changes in the kidney that lead to appropriate changes in glomerular filtration rate (GFR), particularly when the changes are acute. Furthermore, sCr or BUN cannot change quickly enough with injury since individuals with normal renal function have a functional reserve that is brought into play in response to nephron injury. Other nephrons increase their function so that sCr and BUN may not move out of the "normal range" until there is a great deal of injury and potentially irreversible loss of nephrons. Thus GFR, whether measured by sCr or by more direct methods such as iohexol clearance, is a measure of function of the kidney which is clearly important but which may not move in sync with injury under all circumstances.
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For research use only. Not intended for any clinical use.