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Human Lung Cancer IgA Autoantibody Array (Glass Slide, 30 Targets) (Y0197) (AbAr-0197-YC)

Specifications

Target Array
Lung Cancer IgA Autoantibody Array
Target
14-3-3 theta, CDK2, DKK-1, IMP1, MALAT1, PGP95, Annexin A1, c-Myc (MYC), GBU4-5, IMP2/IGF2BP2/p62, NY-ESO-1, Recoverin, Annexin A2, Cyclin A, HSP70-9B/HSPA9/GRP-75, IMP3, p53, SOX2, CAGE, Cyclin B1, Hu-D, LAMR1, Paxillin, BIRC5, Cathepsin D, Cyclin D1, IMDH2, MAGEA4, PGAM1, Ubiquilin-1
Species Reactivity
Human
Sample Type
Hydrothorax, Serum, Plasma

Applications

Application
Multiplex Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Glass Slide
Detection Assay
Label-based
Protocol Outline
Thoroughly dry the glass slide and block the array surface to prevent non-specific binding.
Incubate the array with both samples and standards.
Incubate with a biotinylated detection antibody cocktail and then with streptavidin-conjugated fluorescent dye.
Carefully disassemble the glass slide and scan the slide using a gene microarray laser scanner.
Conduct densitometric analysis.
Components
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction
Lung cancer, also known as lung carcinoma, is a malignant lung tumor characterized by uncontrolled cell growth in tissues of the lung. This growth can spread beyond the lung by the process of metastasis into nearby tissue or other parts of the body.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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