What sample types are compatible with the Human/Mouse MAPK Phosphorylation Antibody Array (Y0188)?

This array is designed for use with cell lysates and tissue lysates from both human and mouse samples. It is important to prepare lysates using a lysis buffer that effectively preserves protein phosphorylation, typically containing phosphatase inhibitors.

How much protein sample is required per array?

The optimal protein amount may vary depending on the sample type and the abundance of the target proteins. We recommend starting with the amount suggested in the product manual and optimizing as needed. Typically, a few hundred micrograms of total protein per array is sufficient.

Human/Mouse MAPK Phosphorylation Antibody Array (Membrane, 17 Targets) (Y0188) (AbAr-0188-YC)

See all MAPK Phosphorylation antibodies

Online Inquiry

Datasheet

Specifications

Target Array

MAPK Phosphorylation Array

Target

Akt (pSer473) (PKB (pSer473)), HSP27 (pSer82), MSK2 (pSer360), RSK1 (pSer380), CREB (pSer133), JNK (pThr183), mTOR (pSer2448), RSK2 (pSer386), ERK1 (pThr202, pTyr204)/ERK2 (pTyr185, pTyr187), MEK (pSer217/pSer221), p38 (pThr180, pTyr182), GSK3a (pSer21), MKK3 (pSer189), P53 (pSer15), GSK3b (pSer9), MKK6 (pSer207), P70S6K (pThr421, pSer424)

Species Reactivity

Human, Mouse

Sample Type

Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates

Applications

Application

Multiplex Protein Detection

Assay Type

Semi-Quantitative

Solid Support

Membrane

Detection Assay

Sandwich-based

Protocol Outline

Conduct membrane blocking procedures.
Incubate with the provided sample.
Incubate using the detection antibody cocktail.
Proceed with incubation using HRP-anti-IgG antibodies.
Engage in incubation with detection buffer solutions.
Capture images utilizing a chemiluminescent imaging system.
Perform densitometric analysis and data interpretation.

Components

Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction

A mitogen-activated protein kinase (MAPK or MAP kinase) is a type of protein kinase that is specific to the amino acids serine and threonine (i.e., a serine/threonine-specific protein kinase). MAPKs are involved in directing cellular responses to a diverse array of stimuli, such as mitogens, osmotic stress, heat shock and proinflammatory cytokines. They regulate cell functions including proliferation, gene expression, differentiation, mitosis, cell survival, and apoptosis.

More Infomation

Q & As

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Review & reward

Have you used Human/Mouse MAPK Phosphorylation Antibody Array (Membrane, 17 Targets) (Y0188)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email: