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Creative Biolabs has successfully developed SARS, MERS and other anti-viral antibodies in the past to assist scientific research. Facing the difficult COVID-19, Creative Biolabs actively invests in antibody development to assist researchers to better understand the characteristics of 2019-nCoV and drug development. Currently, Creative Biolabs is proud to offer an extensive line of research antibodies to support the study of SARS-CoV-2/COVID-19, several of which were validated using virus-infected cell lysates.

Explore the collections of SARS-CoV-2/COVID-19 antibodies >

Human/Mouse MAPK Phosphorylation Antibody Array (Membrane, 17 Targets) (Y0188) (AbAr-0188-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Human/Mouse MAPK Phosphorylation Antibody Array detects 17 phosphorylated proteins in human or mouse samples. It is suitable for all liquid sample types but intended for use with cell and tissue lysates.
Specifications
Target Array
MAPK Phosphorylation Array
Target
Akt (P-Ser473) (PKB (P-Ser473)), HSP27 (P-Ser82), MSK2 (P-Ser360), RSK1 (P-Ser380), CREB (P-Ser133), JNK (P-Thr183), mTOR (P-Ser2448), RSK2 (P-Ser386), ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187), MEK (P-Ser217/Ser221), p38 (P-Thr180/Tyr182), GSK3a (P-Ser21), MKK3 (P-Ser189), P53 (P-Ser15), GSK3b (P-Ser9), MKK6 (P-Ser207), P70S6K (P-Thr421/Ser424)
Species Reactivity
Human, Mouse
Sample Type
Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates
Applications
Application
Multiplex Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Membrane
Detection Assay
Sandwich-based
Protocol Outline
Block membranes
Incubate with Sample
Incubate with Detection Antibody Cocktail
Incubate with HRP-Conjugated anti-IgG
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.
Background
Introduction
A mitogen-activated protein kinase (MAPK or MAP kinase) is a type of protein kinase that is specific to the amino acids serine and threonine (i.e., a serine/threonine-specific protein kinase). MAPKs are involved in directing cellular responses to a diverse array of stimuli, such as mitogens, osmotic stress, heat shock and proinflammatory cytokines. They regulate cell functions including proliferation, gene expression, differentiation, mitosis, cell survival, and apoptosis.
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