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Human NFkB Pathway Antibody Array (Membrane, 41 Targets) (Y0248) (AbAr-0248-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Human NFkB Pathway Antibody Array detects 41 human proteins and 4 serine or tyrosine phosphorylation sites simultaneously.
Datasheet SDS
Specifications
Target Array
NFkB Pathway Array
Target
ASC, IL-18 R alpha, RelA/p65 (pS529), BCL-10, IRAK1, c-Rel, CARD6, IRF5, SHARPIN, CD40/TNFRSF5, IRF8, SOCS-6, dAP1/BIRC2, JNK1/2, STAT1p91, dAP2/BIRC3, JNK2, STAT1 (pY701), FADD/MORT1, LTBR/TNFRSF3, STAT2, Fas/TNFRSF6/CD95, Metadherin/AEG-1, STAT2 (pY689), IκBα, MYD88, STING/TMEM173, IκBε, NFκB1, TLR2, IKK1/IKKα/CHUK, NFκB2, TNF RI/TNFRSF1A, IKK2/IKKβ, NGF R/TNFRSF16, TNF RII/TNFRSF1B, IKKυ/NEMO, P53, TRAF2, IL-1 RI, P53 (pS46), TRAIL R1/DR4, IL-17 RA, RelA/p65, TRAIL R2/DR5
Species Reactivity
Human
Sample Type
Cell Lysates
Applications
Application
Multiplex Protein Detection
Solid Support
Membrane
Detection Assay
Sandwich-based
Protocol Outline
Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.
Background
Introduction
NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) is a protein complex that controls transcription of DNA, cytokine production and cell survival. NF-κB is found in almost all animal cell types and is involved in cellular responses to stimuli such as stress, cytokines, free radicals, heavy metals, ultraviolet irradiation, oxidized LDL, and bacterial or viral antigens. NF-κB plays a key role in regulating the immune response to infection. Incorrect regulation of NF-κB has been linked to cancer, inflammatory and autoimmune diseases, septic shock, viral infection, and improper immune development. NF-κB has also been implicated in processes of synaptic plasticity and memory.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

For Research Use Only. Not For Clinical Use.
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