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Human NFkB Pathway Antibody Array (Membrane, 41 Targets) (Y0248) (AbAr-0248-YC)

Specifications

Target Array
NFkB Pathway Array
Target
ASC, IL-18 R alpha, RelA/p65 (pS529), BCL-10, IRAK1, c-Rel, CARD6, IRF5, SHARPIN, CD40, IRF8, SOCS-6, dAP1/BIRC2, JNK1/2, STAT1p91, dAP2/BIRC3, JNK2, STAT1 (pY701), FADD/MORT1, LTBR/TNFRSF3, STAT2, TNFRSF6/CD95, Metadherin/AEG-1, STAT2 (pY689), IκBα, MYD88, STING/TMEM173, IκBε, NFκB1, TLR2, IKK1/IKKα/CHUK, NFκB2, TNF RI/TNFRSF1A, IKK2/IKKβ, NGF R/TNFRSF16, TNF RII/TNFRSF1B, IKKυ/NEMO, P53, TRAF2, IL-1 RI, P53 (pS46), TRAIL R1/DR4, IL-17 RA, RelA/p65, TRAIL R2/DR5
Species Reactivity
Human
Sample Type
Cell Lysates

Applications

Application
Multiplex Protein Detection
Solid Support
Membrane
Detection Assay
Sandwich-based
Protocol Outline
Apply a blocking agent to the membranes.
Proceed with sample incubation.
Incubate using the cocktail of biotinylated detection antibodies.
Incubate with streptavidin conjugated to horseradish peroxidase (HRP).
Conduct incubation with the detection buffers.
Utilize a chemiluminescent imaging system for image capture.
Execute densitometric evaluation and perform data analysis.
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction
NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) is a protein complex that controls transcription of DNA, cytokine production and cell survival. NF-κB is found in almost all animal cell types and is involved in cellular responses to stimuli such as stress, cytokines, free radicals, heavy metals, ultraviolet irradiation, oxidized LDL, and bacterial or viral antigens. NF-κB plays a key role in regulating the immune response to infection. Incorrect regulation of NF-κB has been linked to cancer, inflammatory and autoimmune diseases, septic shock, viral infection, and improper immune development. NF-κB has also been implicated in processes of synaptic plasticity and memory.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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