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Human Phospho-Immunoreceptor Antibody Array (Membrane, 59 Targets) (Y0242) (AbAr-0242-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Human Phospho-Immunoreceptor Antibody Array detects phosphorylation of 59 human ITAM/ITIM-associated immunoreceptors simultaneously.
Specifications
Target Array
Phospho-Immunoreceptor Array
Target
2B4/SLAMF4, FcRH1/IRTA5, NKp46/NCR1, BLAME/SLAMF8, FcRH2/IRTA4, NKp80/KLRF1, BTLA, FcRH4/IRTA1, NTB-A/SLAMF6, CD3 epsilon, FcRH5/IRTA2, PD-1, CD5, ILT2/CD85j, PECAM/CD31), CD6, ILT3/CD85k, SHIP-1, CD28, ILT4/CD85d, SHP-1, CD84/SLAMF5, ILT5/CD85a, SHP-2, CD229/SLAMF3, ILT6/CD85e, Siglec-2/CD22, CEACAM-1, Integrin beta 3/CD61, Siglec-3/CD33, CLEC-1, KIR2DL4, Siglec-5, CLEC-2, LAIR-1, Siglec-7, CRACC/SLAMF7, LAIR-2, Siglec-9, CTLA-4/CD152, LMIR1/CD300A, Siglec-10, DCIR/CLEC4A, LMIR2/CD300C, SIRP-beta 1, Dectin-1/CLEC7A, LMIR3/CD300LF, SLAM/CD150, DNAM-1, LMIR6/CD300LE, TREM-1, Fc epsilon RII/CD23, MDL-1/CLEC5A, TREM-2, Fc gamma RIIA, NKp30/NCR3, TREML1/TLT-1, Fc gamma RIIIA/B, NKp44/NCR2
Species Reactivity
Human
Sample Type
Cell Lysates
Applications
Application
Multiplex Protein Detection
Solid Support
Membrane
Detection Assay
Sandwich-based
Protocol Outline
Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.
Background
Introduction
Immune cells are activated as a result of productive interactions between ligands and various receptors known as immunoreceptors. These receptors function by recruiting cytoplasmic protein tyrosine kinases, which trigger a unique phosphorylation signal leading to cell activation. In the recent past, there has been increasing interest in elucidating the processes involved in the negative regulation of immunoreceptor-mediated signal transduction. Evidence is accumulating that immunoreceptor signaling is inhibited by complex and highly regulated mechanisms that involve receptors, protein tyrosine kinases, protein tyrosine phosphatases, lipid phosphatases, ubiquitin ligases, and inhibitory adaptor molecules. Genetic evidence indicates that this inhibitory machinery is crucial for normal immune cell homeostasis.
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For Research Use Only. Not For Clinical Use.