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Human Phospho-Kinase Antibody Array (Membrane, 43 Targets) (Y0225) (AbAr-0225-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Human Phospho-Kinase Antibody Array detects phosphorylation of 43 human kinases and total amounts of 2 related proteins simultaneously.
See all Phospho-Kinase antibodies

Specifications

Target Array
Phospho-Kinase Array
Target
Akt 1/2/3 (S473), Hck (Y411), PLC gamma-1 (Y783), Akt 1/2/3 (T308), HSP27 (S78/S82), PRAS40 (T246), AMPK alpha1 (T183), HSP60, Pyk2 (Y402), AMPK alpha2 (T172), JNK 1/2/3 (T183/Y185, T221/Y223), RSK1/2/3 (S380), beta-Catenin, Lck (Y394), Src (Y419), Chk-2 (T68), Lyn (Y397), STAT2 (Y689), c-Jun (S63), MSK1/2 (S376/S360), STAT3 (S727), CREB (S133), p27 (T198), STAT3 (Y705), EGF R (Y1086), p38 alpha (T180/Y182), STAT5a (Y699), eNOS (S1177), p53 (S15), STAT5a/b (Y699), ERK1/2 (T202/Y204, T185/Y187), p53 (S392), STAT5b (Y699), FAK (Y397), p53 (S46), STAT6 (Y641), Fgr (Y412), P70 S6 Kinase (T389), TOR (S2448), Fyn (Y420), p70 S6 Kinase (T421/S424), WNK-1 (T60), GSK-3 alpha/beta (S21/S9), PDGF R beta (Y751), Yes (Y426)
Species Reactivity
Human
Sample Type
Cell Lysates

Applications

Application
Multiplex Protein Detection
Solid Support
Membrane
Detection Assay
Sandwich-based
Protocol Outline
Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction
A protein kinase is a kinase enzyme that modifies other molecules, mostly proteins, by chemically adding phosphate groups to them (phosphorylation). Phosphorylation usually results in a functional change of the target protein (substrate) by changing enzyme activity, cellular location, or association with other proteins. The human genome contains about 518 protein kinase genes and they constitute about 2% of all human genes. Up to 30% of all human proteins may be modified by kinase activity[citation needed], and kinases are known to regulate the majority of cellular pathways, especially those involved in signal transduction. Protein kinases are also found in bacteria and plants, and include the pseudokinase sub-family, which exhibit unusual features including atypical nucleotide binding and weak, or no, catalytic activity and are part of a much larger pseudoenzyme group of 'degraded' enzyme relatives that are found throughout life, where they take an active participation in mechanistic cellular signaling.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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