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Human Ubiquitin Antibody Array (Membrane, 49 Targets) (Y0249) (AbAr-0249-YC)


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Specifications

Target Array
Ubiquitin Array
Target
A20/TNFAIP3, ATF4, Bcl-2, beta-TrCP1/BTRC, CASP8, CBL, Cyclin D1/CCND1, CD44, cIAP-1/HIAP-2, cIAP-2/HIAP-1, COX-2/PTGS2, EGF R, ER-alpha/NR3A1, ErbB2, ErbB3, ErbB4, FASN, F-box protein 15/FBXO15, FBXW7/Cdc4, FGF R2, HGF R/c-met, HIF-1 alpha, HSP70, HSP90AA1/HSP90a, IGF-I R, I kappa B alpha, I kappa B eplison, IKK gamma/NEMO, Insulin R, IRAK1, IRF3, IRS1, M-CSF R, MSP R/Ron, Nrf2/NFE2L2, NIK/MAP3K14, p21CDKN1A/Cip1, p53, PDGF R alpha, PDGF R beta, RIP1, SCF R, TfR, TNF R1/TNFRSF1A, TRAF-2, TRAF-3, TRAF-6, Trk A, VEGF R3
Species Reactivity
Human

Applications

Application
Multiplex Protein Detection
Solid Support
Membrane
Detection Assay
Sandwich-based
Protocol Outline
Apply a blocking agent to the membranes.
Proceed with sample incubation.
Incubate using the cocktail of biotinylated detection antibodies.
Incubate with streptavidin conjugated to horseradish peroxidase (HRP).
Conduct incubation with the detection buffers.
Utilize a chemiluminescent imaging system for image capture.
Execute densitometric evaluation and perform data analysis.
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction
Ubiquitin is a small protein that is found in almost all cellular tissues in humans and other eukaryotic organisms, which helps to regulate the processes of other proteins in the body.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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