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Human Ubiquitin Antibody Array (Membrane, 49 Targets) (Y0249) (AbAr-0249-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. To detect changes in ubiquitination of 49 different proteins between samples.
Specifications
Target Array
Ubiquitin Array
Target
A20/TNFAIP3, ATF4, Bcl-2, beta-TrCP1/BTRC, Caspase-8, CBL, Cyclin D1/CCND1, CD44, cIAP-1/HIAP-2, cIAP-2/HIAP-1, COX-2/PTGS2, EGF R, ER-alpha/NR3A1, ErbB2, ErbB3, ErbB4, FASN, F-box protein 15/FBXO15, FBXW7/Cdc4, FGF R2, HGF R/c-met, HIF-1 alpha, HSP70, HSP90AA1/HSP90a, IGF-I R, I kappa B alpha, I kappa B eplison, IKK gamma/NEMO, Insulin R, IRAK1, IRF3, IRS1, M-CSF R, MSP R/Ron, Nrf2/NFE2L2, NIK/MAP3K14, p21CDKN1A/Cip1, p53, PDGF R alpha, PDGF R beta, RIP1, SCF R/c-Kit, TfR, TNF R1/TNFRSF1A, TRAF-2, TRAF-3, TRAF-6, Trk A, VEGF R3/Flt-4
Species Reactivity
Human
Applications
Application
Multiplex Protein Detection
Solid Support
Membrane
Detection Assay
Sandwich-based
Protocol Outline
Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.
Background
Introduction
Ubiquitin is a small protein that is found in almost all cellular tissues in humans and other eukaryotic organisms, which helps to regulate the processes of other proteins in the body.
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For Research Use Only. Not For Clinical Use.