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Mouse XL Cytokine Antibody Array (Membrane, 111 Targets) (Y0231) (AbAr-0231-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Mouse XL Cytokine Antibody Array detects 111 mouse cytokines simultaneously.
Specifications
Target Array
XL Cytokine Array
Target
Adiponectin/Acrp30, DPPIV/CD26, IL-27, Amphiregulin, EGF, IL-28, Angiopoietin-1, Endoglin/CD105, IL-33, Angiopoietin-2, Endostatin, LDL R, Angiopoietin-like 3, Fetuin A/AHSG, Leptin, BAFF/BLyS/TNFSF13B, FGF acidic, LIF, C1q R1/CD93, FGF-21, Lipocalin-2/NGAL, CCL2/JE/MCP-1, Flt-3 Ligand, LIX, CCL3/CCL4 MIP-1 alpha/beta, Gas6, M-CSF, CCL5/RANTES, G-CSF, MMP-2, CCL6/C10, GDF-15, MMP-3, CCL11/Eotaxin, GM-CSF, MMP-9, CCL12/MCP-5, HGF, Myeloperoxidase, CCL17/TARC, ICAM-1/CD54, Osteopontin (OPN), CCL19/MIP-3 beta, IFN-gamma, Osteoprotegerin/TNFRSF11B, CCL20/MIP-3 alpha, IGFBP-1, PD-ECGF/Thymidine phosphorylase, CCL21/6Ckine, IGFBP-2, PDGF-BB, CCL22/MDC, IGFBP-3, Pentraxin 2/SAP, CD14, IGFBP-5, Pentraxin 3/ TSG-14, CD40/TNFRSF5, IGFBP-6, Periostin/OSF-2, CD160, IL-1 alpha/IL1F1, Pref-1/DLK-1/FA1, Chemerin, IL-1 beta/IL-1F2, Proliferin, Chitinase 3-like 1, IL-1ra/IL-1F3, Proprotein Convertase 9/PCSK9, Coagulation Factor III/Tissue Factor, IL-2, RAGE, Complement Component C5/C5a, IL-3, RBP4, Complement Factor D, IL-4, Reg3G, C-Reactive Protein/CRP, IL-5, Resistin, CX3CL1/Fractalkine, IL-6, E-Selectin/CD62E, CXCL1/KC, IL-7, P-Selectin/CD62P, CXCL2/MIP-2, IL-10, Serpin E1/PAI-1, CXCL9/MIG, IL-11, Serpin F1/PEDF, CXCL10/IP-10, IL-12p40, Thrombopoietin, CXCL11/I-TAC, IL-13, TIM-1/KIM-1/HAVCR, CXCL13/BLC/BCA-1, IL-15, TNF-alpha, CXCL16, IL-17A, VCAM-1/CD106, Cystatin C , IL-22, VEGF, Dkk-1, IL-23, WISP-1/CCN4
Species Reactivity
Mouse
Sample Type
Cell Culture Supernatant, Cell Lysates, Tissue Lysates, Serum, Urine
Applications
Application
Multiplex Protein Detection
Solid Support
Membrane
Detection Assay
Sandwich-based
Protocol Outline
Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.
Background
Introduction
Cytokines are a broad and loose category of small proteins (5–20 kDa) that are important in cell signaling. Cytokines are peptides, and cannot cross the lipid bilayer of cells to enter the cytoplasm. Cytokines have been shown to be involved in autocrine signaling, paracrine signaling and endocrine signaling as immunomodulating agents. Their definite distinction from hormones is still part of ongoing research. Cytokines include chemokines, interferons, interleukins, lymphokines, and tumour necrosis factors, but generally not hormones or growth factors (despite some overlap in the terminology). Cytokines are produced by a broad range of cells, including immune cells like macrophages, B lymphocytes, T lymphocytes and mast cells, as well as endothelial cells, fibroblasts, and various stromal cells; a given cytokine may be produced by more than one type of cell.
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For research use only. Not intended for any clinical use.