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Rabbit Anti-5-caC Recombinant Antibody (V2-623778) (CBMAB-MA265-YC)

Provided herein is a Rabbit monoclonal antibody against 5-carboxylcytosine. The antibody can be used for immunoassay techniques, such as Nucleotide Array, ELISA, MeDIP.
See all 5-caC antibodies
Published Data
Rabbit Anti-5-caC Recombinant Antibody (MA001YC) in Dot blot
Dot blot analysis of genomic 5fC, and 5caC in WT and PROSER1 KO HEK293 cells. 5fC, and 5caC are decreased in PROSER1 KO versus WT cells. Methylene Blue staining was performed to ensure that equal amounts of DNA were used from WT and PROSER1 KO cells. ...View More
Wang, X., Rosikiewicz, W., Sedkov, Y., Martinez, T., Hansen, B. S., Schreiner, P., ... & Herz, H. M. (2022). PROSER1 mediates TET2 O-GlcNAcylation to regulate DNA demethylation on UTX-dependent enhancers and CpG islands. Life Science Alliance, 5(1).
Rabbit Anti-5-caC Recombinant Antibody (MA001YC) in Dot blot
5caC dot blot in WT and Tdg−/− mESC genomic DNA. Replicates as in (B) with methylene blue staining as loading control. Relative 5caC abundance when compared with WT control is determined through methylene blue normalization. ...View More
Nanan, K. K., Sturgill, D. M., Prigge, M. F., Thenoz, M., Dillman, A. A., Mandler, M. D., & Oberdoerffer, S. (2019). TET-catalyzed 5-carboxylcytosine promotes CTCF binding to suboptimal sequences genome-wide. Iscience, 19, 326-339.

Summary

Host Animal
Rabbit
Clone
V2-623778
Antibody Isotype
IgG
Application
Nucleotide Array, ELISA, MeDIP

Basic Information

Immunogen
5-carboxylcytidine conjugated to keyhole limpet haemocyan (KLH)
Host Species
Rabbit
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
HEPES pH 7.5, 0.01% BSA, 50% Glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

Target

Full Name
5-carboxylcytosine
Introduction
5-carboxylcytosine (5caC) is the final oxidized derivative of 5-methylcytosine (5mC). 5mC is oxidized to 5-hydroxymethylcytosine (5hmC) which is then oxidized to 5-formylcytosine (5fC) then 5caC. Each of these oxidation steps are catalyzed by the Ten-Eleven Translocation (TET) family of enzymes. 5fC can then be further oxidized to 5-carboxylcytosine (5caC) by TET. Both 5fC and 5caC can be converted to unmodified cytosine by Terminal deoxynucleotidyl transferase (TdT) or Thymine DNA glycosylase (TDG) by base excision repair.
Alternative Names
5-carboxylcytosine
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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