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Mouse Anti-ABCG5 Recombinant Antibody (V2-179093) (CBMAB-A0330-YC)

Provided herein is a Mouse monoclonal antibody against Human ATP Binding Cassette Subfamily G Member 5. The antibody can be used for immunoassay techniques, such as ELISA, FC, IHC-P, WB.
See all ABCG5 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-179093
Antibody Isotype
IgG1
Application
ELISA, FC, IHC-P, WB

Basic Information

Immunogen
Partial recombinant human ABCG5 (between residues 300-500) expressed in E. coli.
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:500-1:2,000
IHC-P1:200-1:2,000
IHC1:200-1:2,000
FC1:200-1:400
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
PBS, 0.05% sodium azide, 0.5% protein stabilizer
Buffer
PBS, 0.5% proprietary protein stabilizer
Preservative
0.05% sodium azide
Concentration
1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 300-500

Target

Full Name
ATP Binding Cassette Subfamily G Member 5
Introduction
ABCG5 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN
Entrez Gene ID
64240
UniProt ID
Q9H222
Alternative Names
ATP Binding Cassette Subfamily G Member 5; ATP-Binding Cassette, Sub-Family G (WHITE), Member 5; Sterolin 1; ATP-Binding Cassette Sub-Family G Member 5; Sterolin-1; STSL;
Function
ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg2+- and ATP-dependent sterol transport across the cell membrane. Plays an essential role in the selective transport of dietary plant sterols and cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. Required for normal sterol homeostasis. The heterodimer with ABCG8 has ATPase activity.
Biological Process
Bile acid signaling pathway
Cholesterol efflux
Cholesterol homeostasis
Excretion
Intestinal cholesterol absorption
Intracellular receptor signaling pathway
Lipid transport
Negative regulation of intestinal cholesterol absorption
Negative regulation of intestinal phytosterol absorption
Response to drug
Response to ionizing radiation
Response to muscle activity
Response to nutrient
Sterol transport
Triglyceride homeostasis
Cellular Location
Cell membrane; Apical cell membrane
Involvement in disease
A form of sitosterolemia, an autosomal recessive metabolic disorder characterized by unregulated intestinal absorption of cholesterol, phytosterols and shellfish sterols, and decreased biliary excretion of dietary sterols into bile. Patients have hypercholesterolemia, very high levels of plant sterols in the plasma, and frequently develop tendon and tuberous xanthomas, accelerated atherosclerosis and premature coronary artery disease.
Topology
Cytoplasmic: 1-383 aa
Helical: 384-404 aa
Extracellular: 405-421 aa
Helical: 422-442 aa
Cytoplasmic: 443-467 aa
Helical: 468-489 aa
Extracellular: 490-500 aa
Helical: 501-521 aa
Cytoplasmic: 522-528 aa
Helical: 529-549 aa
Extracellular: 550-623 aa
Helical: 624-644 aa
Cytoplasmic: 645-651 aa
PTM
N-glycosylated.

Fashe, M., Yi, M., Sueyoshi, T., & Negishi, M. (2021). Sex-specific expression mechanism of hepatic estrogen inactivating enzyme and transporters in diabetic women. Biochemical Pharmacology, 114662.

Yamada, Y., Sugi, K., Gatate, Y., Senbonmatsu, T., Inoue, I., Fukushima, K., ... & Tada, H. (2021). Premature acute myocardial infarction in a young patient with sitosterolemia. CJC Open.

Nishikawa, R., Furuhashi, M., Hori, M., Ogura, M., Harada-Shiba, M., Okada, T., ... & Miura, T. (2021). A Resuscitated Case of Acute Myocardial Infarction with both Familial Hypercholesterolemia Phenotype Caused by Possibly Oligogenic Variants of the PCSK9 and ABCG5 Genes and Type I CD36 Deficiency. Journal of Atherosclerosis and Thrombosis, 58909.

Nakano, Y., Komiya, C., Shimizu, H., Mishima, H., Shiba, K., Tsujimoto, K., ... & Yamada, T. (2020). A case of ezetimibe-effective hypercholesterolemia with a novel heterozygous variant in ABCG5. Endocrine Journal, EJ20-0044.

Zein, A. A., Kaur, R., Hussein, T. O., Graf, G. A., & Lee, J. Y. (2019). ABCG5/G8: a structural view to pathophysiology of the hepatobiliary cholesterol secretion. Biochemical society transactions, 47(5), 1259-1268.

Mikhailova, S., Ivanoshchuk, D., Timoshchenko, O., & Shakhtshneider, E. (2019). Genes potentially associated with familial hypercholesterolemia. Biomolecules, 9(12), 807.

Tada, H., Nomura, A., Yamagishi, M., & Kawashiri, M. A. (2018). First case of sitosterolemia caused by double heterozygous mutations in ABCG5 and ABCG8 genes. Journal of clinical lipidology, 12(5), 1164-1168.

Ono, S., Matsuda, J., Saito, A., Yamamoto, T., Fujimoto, W., Shimizu, H., ... & Ouchi, K. (2017). A case of sitosterolemia due to compound heterozygous mutations in ABCG5: clinical features and treatment outcomes obtained with colestimide and ezetimibe. Clinical Pediatric Endocrinology, 26(1), 17-23.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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