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Mouse Anti-Acrolein Recombinant Antibody (V2-634090) (CBMAB-AP093LY)


See all Acrolein antibodies
Published Data
Mouse Anti-Acrolein Recombinant Antibody (2H2) in IF
Visualizing the elevation of acrolein-Lys adducts as a function of single-impact severity. Representative fluorescence images at ×20 magnification are shown for histiotypical networks that received single impact forces of (A) 0 (control), (B) 30 g, (C) 100 g, (D) and 200 g. Nuclei are visualized with DAPI (blue), neuronal cell bodies and processes with neuron specific Neurofilament (green), and acrolein-Lys adducts with Stressmarq (red). Levels of acrolein-Lys adducts (red) progressively increase with larger g forces, moving top-to-bottom (A-D). Images demonstrating the spatial distribution of acrolein elevation are shown in both neuronal (E1-3) and non-neuronal cells (E4-5) which are enlarged from the (D)-merge series (200 g impact). Note the effective labeling of these antibodies, with the clear majority of all fluorescence being associated with cells, accompanied by very little background noise from our substrate and media. Further, these results suggest that all cells in our histiotypical cultures, both neuron (E1-3) and non-neuron (i.e. glia, E4&5), show increases in acrolein production, participating in the pathological consequences. However, most acrolein production appears to be perinuclear, with the majority localized in cell bodies (E1,4,5). While diffusion into cellular processes is observed (E2&3), it seems to be much less prevalent. ...View More
Rogers, E. A., Beauclair, T., Thyen, A., & Shi, R. (2022). Utilizing novel TBI-on-a-chip device to link physical impacts to neurodegeneration and decipher primary and secondary injury mechanisms. Scientific Reports, 12(1), 11838.

Summary

Host Animal
Mouse
Clone
V2-634090
Antibody Isotype
IgG1
Application
ICC/IF, ELISA, FC, WB

Basic Information

Immunogen
Synthetic Acrolein modified Keyhole Limpet Hemocyanin (KLH).
Host Species
Mouse
Antibody Isotype
IgG1
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
ELISA1:1,000
IF(ICC)1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4, 50% Glycerol, 0.05% BSA
Preservative
0.09% sodium azide
Concentration
1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Acrolein
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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