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Rabbit Anti-AIF1 Recombinant Antibody (V2-266302) (CBMAB-I1284-YY)

This product is Rabbit antibody that recognizes AIF1. The antibody CBYY-I0582 can be used for immunoassay techniques such as: IHC-P, FC, IF
See all AIF1 antibodies

Summary

Host Animal
Rabbit
Specificity
Mouse, Rat, Human
Clone
V2-266302
Antibody Isotype
IgG
Application
ELISA, WB, IP

Basic Information

Immunogen
Recombinant Human IBA1 / AIF1 protein.
Host Species
Rabbit
Specificity
Mouse, Rat, Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
ELISA1:5,000-1:10,000
WB1:500-1:2,000
IP1-4 μl/mg of lysate

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
None
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Allograft Inflammatory Factor 1
Introduction
This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain.
Entrez Gene ID
Human199
Mouse11629
Rat29427
UniProt ID
HumanP55008
MouseO70200
RatP55009
Alternative Names
Allograft Inflammatory Factor 1; Ionized Calcium-Binding Adapter Molecule 1; Interferon Gamma Responsive Transcript; Protein G1; AIF-1; IBA1; IRT-1; IRT1; G1;
Function
Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
Biological Process
Actin crosslink formation Source: ARUK-UCL
Actin filament bundle assembly Source: UniProtKB
Actin filament polymerization Source: UniProtKB
Cellular response to interferon-gamma Source: UniProtKB
Cellular response to oxidative stress Source: ARUK-UCL
Inflammatory response Source: UniProtKB
Microglial cell activation Source: UniProtKB
Negative regulation of smooth muscle cell chemotaxis Source: UniProtKB
Negative regulation of smooth muscle cell proliferation Source: UniProtKB
Parallel actin filament bundle assembly Source: ARUK-UCL
Phagocytosis, engulfment Source: UniProtKB
Positive regulation of cell migration Source: ARUK-UCL
Positive regulation of cell population proliferation Source: ARUK-UCL
Positive regulation of chemokine production Source: ARUK-UCL
Positive regulation of chemotaxis Source: ARUK-UCL
Positive regulation of fibroblast growth factor production Source: ARUK-UCL
Positive regulation of G1/S transition of mitotic cell cycle Source: UniProtKB
Positive regulation of interleukin-6 production Source: ARUK-UCL
Positive regulation of monocyte chemotaxis Source: UniProtKB
Positive regulation of mononuclear cell migration Source: ARUK-UCL
Positive regulation of smooth muscle cell chemotaxis Source: UniProtKB
Positive regulation of smooth muscle cell proliferation Source: UniProtKB
Positive regulation of T cell migration Source: UniProtKB
Positive regulation of T cell proliferation Source: UniProtKB
Rac protein signal transduction Source: UniProtKB
Regulation of gene expression Source: ARUK-UCL
Ruffle assembly Source: UniProtKB
Cellular Location
Ruffle membrane; Cytoplasmic side; Cytoskeleton; Phagocytic cup. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.
PTM
Phosphorylated on serine residues.

da Silva, R. L., Elizondo, D. M., Brandy, N. Z., Haddock, N. L., Boddie, T. A., de Oliveira, L. L., ... & Lipscomb, M. W. (2021). Leishmania donovani infection suppresses Allograft Inflammatory Factor-1 in monocytes and macrophages to inhibit inflammatory responses. Scientific reports, 11(1), 1-10.

Sikora, M., Kopeć, B., Piotrowska, K., & Pawlik, A. (2020). Role of allograft inflammatory factor-1 in pathogenesis of diseases. Immunology letters, 218, 1-4.

Vizioli, J., Verri, T., & Pagliara, P. (2020). Allograft inflammatory factor-1 in metazoans: Focus on invertebrates. Biology, 9(11), 355.

Egaña-Gorroño, L., Chinnasamy, P., Casimiro, I., Almonte, V. M., Parikh, D., Oliveira-Paula, G. H., ... & Sibinga, N. E. (2019). Allograft inflammatory factor-1 supports macrophage survival and efferocytosis and limits necrosis in atherosclerotic plaques. Atherosclerosis, 289, 184-194.

Elizondo, D. M., Brandy, N. Z., da Silva, R. L., Haddock, N. L., Kacsinta, A. D., de Moura, T. R., & Lipscomb, M. W. (2019). Allograft inflammatory factor-1 governs hematopoietic stem cell differentiation into cDC1 and monocyte-derived dendritic cells through IRF8 and RelB in vitro. Frontiers in immunology, 10, 173.

Zhou, Y., Li, X., Yuan, X., & Hao, L. (2019). Role of allograft inflammatory factor‐1 in the regulation of inflammation and oxidative stress in primary peritoneal mesothelial cells. Cell biology international, 43(5), 495-505.

Donovan, K. M., Leidinger, M. R., McQuillen, L. P., Goeken, J. A., Hogan, C. M., Harwani, S. C., ... & Meyerholz, D. K. (2018). Allograft inflammatory factor 1 as an immunohistochemical marker for macrophages in multiple tissues and laboratory animal species. Comparative medicine, 68(5), 341-348.

Ren, J., Lin, Y., Tang, J., Yue, H., & Zhao, Y. (2018). Allograft inflammatory factor-1 mediates macrophage-induced impairment of insulin signaling in adipocytes. Cellular Physiology and Biochemistry, 47(1), 403-413.

Elizondo, D. M., Andargie, T. E., Yang, D., Kacsinta, A. D., & Lipscomb, M. W. (2017). Inhibition of allograft inflammatory factor-1 in dendritic cells restrains CD4+ T cell effector responses and induces CD25+ Foxp3+ T regulatory subsets. Frontiers in immunology, 8, 1502.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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