Mouse Anti-ALKBH3 Recombinant Antibody (2A5-4F5) (CBMAB-A2186-LY)

Name: Mouse Anti-ALKBH3 Recombinant Antibody (2A5-4F5)
SKU: CBMAB-A2186-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2A5-4F5

Application

WB, ELISA

Immunogen

DEPC-1 (AAH15155, 1 a.a. ~ 139 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

AlkB Homolog 3, Alpha-Ketoglutaratedependent Dioxygenase

Introduction

The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM

Entrez Gene ID

221120

UniProt ID

Q96Q83

Alternative Names

ABH3; DEPC-1; DEPC1; MGC118790; MGC118792; MGC118793; PCA1

Function

Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A) (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:26863196, PubMed:26863410). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:25944111). Has a strong preference for single-stranded DNA (PubMed:12486230, PubMed:12594517, PubMed:16174769). Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3 (PubMed:22055184). Also acts on RNA (PubMed:12594517, PubMed:16174769, PubMed:26863196, PubMed:26863410, PubMed:16858410). Demethylates N1-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons (PubMed:26863196, PubMed:26863410). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:22055184, PubMed:16858410).

Biological Process

Cell population proliferation Source: UniProtKB
DNA dealkylation involved in DNA repair Source: UniProtKB
DNA repair Source: UniProtKB
Oxidative single-stranded DNA demethylation Source: UniProtKB
Oxidative single-stranded RNA demethylation Source: UniProtKB

Cellular Location

Nucleus; Cytoplasm. Colocalizes with ASCC2 and ASCC3 in nuclear foci when cells have been exposed to alkylating agents that cause DNA damage (PubMed:29144457). Predominantly localizes to the nucleus.

PTM

Ubiquitinated; undergoes 'Lys-48'-linked polyubiquitination. OTUD4 promotes USP7 and USP9X-dependent deubiquitination of 'Lys-48'-polyubiquitinated ALKBH3 promoting the repair of alkylated DNA lesions.

HU, J. Y., ZHANG, L., & ZHANG, L. (2021). Function of human nucleic acid alkylation damage repair enzyme ALKBH3 in cancer progression and oncotherapy. JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE), 41(5), 684.

Kanazhevskaya, L. Y., Smyshlyaev, D. A., & Fedorova, O. S. (2020). Conformational dynamics in methylated DNA repairby human Fe (II)/alpha-ketoglutarate dependent dioxygenases ALKBH2 and ALKBH3. In BIOINFORMATICS OF GENOME REGULATION AND STRUCTURE/SYSTEMS BIOLOGY (BGRS/SB-2020) (pp. 576-576).

Ueda, M., Shimizu, T., Mabuchi, M., Horiike, K., Kitae, K., Hase, H., ... & Tanaka, A. (2018). Novel metabolically stable PCA-1/ALKBH3 inhibitor has potent antiproliferative effects on DU145 cells in vivo. Anticancer research, 38(1), 211-218.

Nigam, R., Babu, K. R., Ghosh, T., Kumari, B., Akula, D., Rath, S. N., ... & Khan, F. A. (2018). Indenone derivatives as inhibitor of human DNA dealkylation repair enzyme AlkBH3. Bioorganic & medicinal chemistry, 26(14), 4100-4112.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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