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Mouse Anti-ALKBH3 Recombinant Antibody (B7) (CBMAB-P0040-YC)

Provided herein is a Mouse monoclonal antibody against Human AlkB Homolog 3, Alpha-Ketoglutaratedependent Dioxygenase. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all ALKBH3 antibodies
Published Data
Mouse Anti-ALKBH3 Recombinant Antibody (B7) in IF
HeLa cells transfected with P-body marker CFP-DCP1A were treated for 1 h as indicated, fixed, and double stained with anti-ASCC3 (red) and anti-ALKBH3 (green). ...View More
Wollen, K. L., Hagen, L., Vågbø, C. B., Rabe, R., Iveland, T. S., Aas, P. A., ... & Slupphaug, G. (2021). ALKBH3 partner ASCC3 mediates P-body formation and selective clearance of MMS-induced 1-methyladenosine and 3-methylcytosine from mRNA. Journal of Translational Medicine, 19(1), 1-22.
Mouse Anti-ALKBH3 Recombinant Antibody (B7) in Immunoblot
Immunoblot shows ALKBH3 overexpression (OE) increases CSF-1 protein, and ALKBH3 silencing (shRNA) decreases CSF-1 protein in Hey cells. ...View More
Woo, H. H., & Chambers, S. K. (2019). Human ALKBH3-induced m1A demethylation increases the CSF-1 mRNA stability in breast and ovarian cancer cells. Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms, 1862(1), 35-46.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
B7
Antibody Isotype
IgG1, κ
Application
ELISA, WB, IP, IF

Basic Information

Immunogen
Amino acids 1-270 mapping at the N-terminus of ALKBH3 of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
AlkB Homolog 3, Alpha-Ketoglutaratedependent Dioxygenase
Introduction
The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine.
Entrez Gene ID
221120
UniProt ID
Q96Q83
Alternative Names
AlkB Homolog 3, Alpha-Ketoglutaratedependent Dioxygenase; Alkylated DNA Repair Protein AlkB Homolog 3; DEPC-1; DEPC1; HABH3; ABH3; AlkB Homolog 3, Alpha-Ketoglutarate-Dependent Dioxygenase; Alpha-Ketoglutarate-Dependent Dioxygenase AlkB Homolog 3;
Function
Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A) (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:26863196, PubMed:26863410). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:25944111). Has a strong preference for single-stranded DNA (PubMed:12486230, PubMed:12594517, PubMed:16174769). Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3 (PubMed:22055184). Also acts on RNA (PubMed:12594517, PubMed:16174769, PubMed:26863196, PubMed:26863410, PubMed:16858410). Demethylates N1-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons (PubMed:26863196, PubMed:26863410). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:22055184, PubMed:16858410).
Biological Process
Cell population proliferation Source: UniProtKB
DNA dealkylation involved in DNA repair Source: UniProtKB
DNA repair Source: UniProtKB
Oxidative single-stranded DNA demethylation Source: UniProtKB
Oxidative single-stranded RNA demethylation Source: UniProtKB
Cellular Location
Nucleus; Cytoplasm. Colocalizes with ASCC2 and ASCC3 in nuclear foci when cells have been exposed to alkylating agents that cause DNA damage (PubMed:29144457). Predominantly localizes to the nucleus.
PTM
Ubiquitinated; undergoes 'Lys-48'-linked polyubiquitination. OTUD4 promotes USP7 and USP9X-dependent deubiquitination of 'Lys-48'-polyubiquitinated ALKBH3 promoting the repair of alkylated DNA lesions.
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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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