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Mouse Anti-ASIC1 Recombinant Antibody (N271-44) (CBMAB-1147-CN)


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Published Data
Mouse Anti-ASIC1 Recombinant Antibody (N271-44) CBMAB-1147-CN in WB
Effect of femoral artery ligation on ASIC1-3 isoform expression levels Western blots for ASIC1 (A), ASIC2 (B) and ASIC3 (C) in DRG tissue isolated from rats that were freely perfused (FP1-FP5) or with ligated femoral arteries (LIG1-LIG4). The 66 and 89 kDa bands in A represent the non-glycosylated and glycosylated forms of ASIC1, respectively. Each lane (loaded with 0.2 μg μl−1 protein) represents one animal. Actin was used as the loading control. The anti-ASIC1, anti-ASIC2, anti-ASIC3 and anti-actin antibodies were used at a dilution of 1:100, 1:200, 1:20 and 1:400, respectively. The plots on the bottom show the mean (±SD) values for ASIC/actin ratios for FP and LIG rats. ∗P = 0.007 and #P = 0.009 for ASIC1 and ASIC3, respectively, employing t tests. ...View More
Farrag, M., Drobish, J. K., Puhl, H. L., Kim, J. S., Herold, P. B., Kaufman, M. P., & Ruiz-Velasco, V. (2017). Endomorphins potentiate acid-sensing ion channel currents and enhance the lactic acid-mediated increase in arterial blood pressure: effects amplified in hindlimb ischaemia. The Journal of physiology, 595(23), 7167-7183.
Mouse Anti-ASIC1 Recombinant Antibody (N271-44) CBMAB-1147-CN in WB
Detection of ASIC1 expression in prostate cancer cell lines by western blot. ...View More
Chen, B., Liu, J., Ho, T. T., Ding, X., & Mo, Y. Y. (2016). ERK-mediated NF-κB activation through ASIC1 in response to acidosis. Oncogenesis, 5(12), e279-e279.

Summary

Host Animal
Mouse
Specificity
Mouse, Rat
Clone
N271-44
Antibody Isotype
IgG1
Application
WB, IHC, ICC

Basic Information

Immunogen
Fusion protein amino acids 460-526 (cytoplasmic C-terminus) of mouse ASIC1 produced recombinantly in E. Coli.
Specificity
Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
10 mM Tris, 50 mM sodium chloride
Preservative
0.065% sodium azide
Concentration
1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Acid Sensing Ion Channel Subunit 1
Introduction
Proton-gated sodium channel; it is activated by a drop of the extracellular pH and then becomes rapidly desensitized. The protein generates a biphasic current with a fast inactivating and a slow sustained phase. It has high selectivity for sodium ions and can also transport lithium ions with high efficiency. It is nearly impermeable to the larger rubidium and cesium ions. This protein mediates glutamate-independent Ca2+ entry into neurons upon acidosis. This Ca2+ overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury.
Entrez Gene ID
Mouse11419
Rat79123
UniProt ID
MouseQ6NXK8
RatP55926
Alternative Names
ASIC; Accn2; BNaC2; ASIC1a; AI843610; B530003N02Rik
Function
Isoform 2 and isoform 3 function as proton-gated sodium channels; they are activated by a drop of the extracellular pH and then become rapidly desensitized. The channel generates a biphasic current with a fast inactivating and a slow sustained phase. Has high selectivity for sodium ions and can also transport lithium ions with high efficiency. Isoform 2 can also transport potassium, but with lower efficiency. It is nearly impermeable to the larger rubidium and cesium ions. Isoform 3 can also transport calcium ions. Mediates glutamate-independent Ca2+ entry into neurons upon acidosis. This Ca2+ overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca2+ concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear.
Isoform 1 does not display proton-gated cation channel activity.
Biological Process
Associative learning Source: Ensembl
Behavioral fear response Source: Ensembl
Calcium ion transmembrane transport Source: Ensembl
Cellular response to pH Source: UniProtKB
Ion transmembrane transport Source: Reactome
Memory Source: Ensembl
Negative regulation of neurotransmitter secretion Source: Ensembl
Protein homotrimerization Source: UniProtKB
Regulation of membrane potential Source: Ensembl
Response to acidic pH Source: Ensembl
Response to amphetamine Source: Ensembl
Response to pH Source: ProtInc
Sensory perception of sour taste Source: UniProtKB
Signal transduction Source: ProtInc
Sodium ion transmembrane transport Source: UniProtKB
Sodium ion transport Source: UniProtKB
Cellular Location
Cell membrane. Localizes in synaptosomes at dendritic synapses of neurons. Colocalizes with DLG4 (By similarity).
Topology
Cytoplasmic: 1-45 aa
Helical: 46-69 aa
Extracellular: 70-427 aa
Discontinuously helical: 428-454 aa
Cytoplasmic: 455-528 aa
PTM
Phosphorylation by PKA regulates interaction with PRKCABP and subcellular location. Phosphorylation by PKC may regulate the channel.
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For research use only. Not intended for any clinical use.

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