Mouse Anti-ASIC1 Recombinant Antibody (N271-44) (CBMAB-1147-CN)

Name: Mouse Anti-ASIC1 Recombinant Antibody (N271-44)
SKU: CBMAB-1147-CN
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ASIC1 Recombinant Antibody (N271-44) CBMAB-1147-CN in WB

Effect of femoral artery ligation on ASIC1-3 isoform expression levels Western blots for ASIC1 (A), ASIC2 (B) and ASIC3 (C) in DRG tissue isolated from rats that were freely perfused (FP1-FP5) or with ligated femoral arteries (LIG1-LIG4). The 66 and 89 kDa bands in A represent the non-glycosylated and glycosylated forms of ASIC1, respectively. Each lane (loaded with 0.2 μg μl−1 protein) represents one animal. Actin was used as the loading control. The anti-ASIC1, anti-ASIC2, anti-ASIC3 and anti-actin antibodies were used at a dilution of 1:100, 1:200, 1:20 and 1:400, respectively. The plots on the bottom show the mean (±SD) values for ASIC/actin ratios for FP and LIG rats. ∗P = 0.007 and #P = 0.009 for ASIC1 and ASIC3, respectively, employing t tests. ...View More

Farrag, M., Drobish, J. K., Puhl, H. L., Kim, J. S., Herold, P. B., Kaufman, M. P., & Ruiz-Velasco, V. (2017). Endomorphins potentiate acid-sensing ion channel currents and enhance the lactic acid-mediated increase in arterial blood pressure: effects amplified in hindlimb ischaemia. The Journal of physiology, 595(23), 7167-7183.

Mouse Anti-ASIC1 Recombinant Antibody (N271-44) CBMAB-1147-CN in WB

Detection of ASIC1 expression in prostate cancer cell lines by western blot. ...View More

Chen, B., Liu, J., Ho, T. T., Ding, X., & Mo, Y. Y. (2016). ERK-mediated NF-κB activation through ASIC1 in response to acidosis. Oncogenesis, 5(12), e279-e279.

Datasheet

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

N271-44

Application

WB, IHC, ICC

Immunogen

Fusion protein amino acids 460-526 (cytoplasmic C-terminus) of mouse ASIC1 produced recombinantly in E. Coli.

Specificity

Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

10 mM Tris, 50 mM sodium chloride

Preservative

0.065% sodium azide

Concentration

1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Acid Sensing Ion Channel Subunit 1

Introduction

Proton-gated sodium channel; it is activated by a drop of the extracellular pH and then becomes rapidly desensitized. The protein generates a biphasic current with a fast inactivating and a slow sustained phase. It has high selectivity for sodium ions and can also transport lithium ions with high efficiency. It is nearly impermeable to the larger rubidium and cesium ions. This protein mediates glutamate-independent Ca2+ entry into neurons upon acidosis. This Ca2+ overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury.

Entrez Gene ID

Mouse	11419
Rat	79123

UniProt ID

Mouse	Q6NXK8
Rat	P55926

Alternative Names

ASIC; Accn2; BNaC2; ASIC1a; AI843610; B530003N02Rik

Function

Isoform 2 and isoform 3 function as proton-gated sodium channels; they are activated by a drop of the extracellular pH and then become rapidly desensitized. The channel generates a biphasic current with a fast inactivating and a slow sustained phase. Has high selectivity for sodium ions and can also transport lithium ions with high efficiency. Isoform 2 can also transport potassium, but with lower efficiency. It is nearly impermeable to the larger rubidium and cesium ions. Isoform 3 can also transport calcium ions. Mediates glutamate-independent Ca2+ entry into neurons upon acidosis. This Ca2+ overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca2+ concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear.
Isoform 1 does not display proton-gated cation channel activity.

Biological Process

Associative learning Source: Ensembl
Behavioral fear response Source: Ensembl
Calcium ion transmembrane transport Source: Ensembl
Cellular response to pH Source: UniProtKB
Ion transmembrane transport Source: Reactome
Memory Source: Ensembl
Negative regulation of neurotransmitter secretion Source: Ensembl
Protein homotrimerization Source: UniProtKB
Regulation of membrane potential Source: Ensembl
Response to acidic pH Source: Ensembl
Response to amphetamine Source: Ensembl
Response to pH Source: ProtInc
Sensory perception of sour taste Source: UniProtKB
Signal transduction Source: ProtInc
Sodium ion transmembrane transport Source: UniProtKB
Sodium ion transport Source: UniProtKB

Cellular Location

Cell membrane. Localizes in synaptosomes at dendritic synapses of neurons. Colocalizes with DLG4 (By similarity).

Topology

Cytoplasmic: 1-45 aa
Helical: 46-69 aa
Extracellular: 70-427 aa
Discontinuously helical: 428-454 aa
Cytoplasmic: 455-528 aa

PTM

Phosphorylation by PKA regulates interaction with PRKCABP and subcellular location. Phosphorylation by PKC may regulate the channel.

Yang, Y. L., & Lai, T. W. (2021). Citric acid in drug formulations causes pain by potentiating acid-sensing ion channel 1. Journal of Neuroscience, 41(21), 4596-4606.

Liu, Y., Ma, J., DesJarlais, R. L., Hagan, R., Rech, J., Lin, D., ... & Maher, M. (2021). Molecular mechanism and structural basis of small-molecule modulation of the gating of acid-sensing ion channel 1. Communications biology, 4(1), 1-15.

Cristofori-Armstrong, B., Saez, N. J., Chassagnon, I. R., King, G. F., & Rash, L. D. (2019). The modulation of acid-sensing ion channel 1 by PcTx1 is pH-, subtype-and species-dependent: Importance of interactions at the channel subunit interface and potential for engineering selective analogues. Biochemical pharmacology, 163, 381-390.

Sun, D., Yu, Y., Xue, X., Pan, M., Wen, M., Li, S., ... & Tian, C. (2018). Cryo-EM structure of the ASIC1a–mambalgin-1 complex reveals that the peptide toxin mambalgin-1 inhibits acid-sensing ion channels through an unusual allosteric effect. Cell discovery, 4(1), 1-11.

Gregory, N. S., Gautam, M., Benson, C. J., & Sluka, K. A. (2018). Acid sensing ion channel 1a (ASIC1a) mediates activity-induced pain by modulation of heteromeric ASIC channel kinetics. Neuroscience, 386, 166-174.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Mouse Anti-ASIC1 Recombinant Antibody (S271-44) (CAT#: CBMAB-AP449LY)

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For research use only. Not intended for any clinical use.

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