Mouse Anti-ASIC1 Recombinant Antibody (S271-44) (CBMAB-AP449LY)

See all ASIC1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

S271-44

Antibody Isotype

IgG1

Application

WB, IHC, ICC

Basic Information

Immunogen

Fusion protein amino acids 460-526 (Cytoplasmic C-terminus) of mouse ASIC1

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH7.4, 50% glycerol

Preservative

0.09% sodium azide

Concentration

1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Acid Sensing Ion Channel Subunit 1

Introduction

This gene encodes a member of the acid-sensing ion channel (ASIC) family of proteins, which are part of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. Members of the ASIC family are sensitive to amiloride and function in neurotransmission. The encoded proteins function in learning, pain transduction, touch sensation, and development of memory and fear. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2012]

Entrez Gene ID

Mouse	11419
Rat	79123

UniProt ID

Mouse	Q6NXK8
Rat	P55926

Alternative Names

Acid Sensing Ion Channel Subunit 1; Amiloride-Sensitive Cation Channel 2, Neuronal; Acid Sensing (Proton Gated) Ion Channel 1; Brain Sodium Channel 2; ACCN2; BNaC2;

Function

Isoform 2 and isoform 3 function as proton-gated sodium channels; they are activated by a drop of the extracellular pH and then become rapidly desensitized. The channel generates a biphasic current with a fast inactivating and a slow sustained phase. Has high selectivity for sodium ions and can also transport lithium ions with high efficiency. Isoform 2 can also transport potassium, but with lower efficiency. It is nearly impermeable to the larger rubidium and cesium ions. Isoform 3 can also transport calcium ions. Mediates glutamate-independent Ca2+ entry into neurons upon acidosis. This Ca2+ overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca2+ concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear.
Isoform 1 does not display proton-gated cation channel activity.

Biological Process

Associative learning Source: Ensembl
Behavioral fear response Source: Ensembl
Calcium ion transmembrane transport Source: Ensembl
Cellular response to pH Source: UniProtKB
Ion transmembrane transport Source: Reactome
Memory Source: Ensembl
Negative regulation of neurotransmitter secretion Source: Ensembl
Protein homotrimerization Source: UniProtKB
Regulation of membrane potential Source: Ensembl
Response to acidic pH Source: Ensembl
Response to amphetamine Source: Ensembl
Response to pH Source: ProtInc
Sensory perception of sour taste Source: UniProtKB
Signal transduction Source: ProtInc
Sodium ion transmembrane transport Source: UniProtKB
Sodium ion transport Source: UniProtKB

Cellular Location

Cell membrane. Localizes in synaptosomes at dendritic synapses of neurons. Colocalizes with DLG4 (By similarity).

Topology

Cytoplasmic: 1-45 aa
Helical: 46-69 aa
Extracellular: 70-427 aa
Discontinuously helical: 428-454 aa
Cytoplasmic: 455-528 aa

PTM

Phosphorylation by PKA regulates interaction with PRKCABP and subcellular location. Phosphorylation by PKC may regulate the channel.

References

Yang, Y. L., & Lai, T. W. (2021). Citric acid in drug formulations causes pain by potentiating acid-sensing ion channel 1. Journal of Neuroscience, 41(21), 4596-4606.

Liu, Y., Ma, J., DesJarlais, R. L., Hagan, R., Rech, J., Lin, D., ... & Maher, M. (2021). Molecular mechanism and structural basis of small-molecule modulation of the gating of acid-sensing ion channel 1. Communications biology, 4(1), 1-15.

Cristofori-Armstrong, B., Saez, N. J., Chassagnon, I. R., King, G. F., & Rash, L. D. (2019). The modulation of acid-sensing ion channel 1 by PcTx1 is pH-, subtype-and species-dependent: Importance of interactions at the channel subunit interface and potential for engineering selective analogues. Biochemical pharmacology, 163, 381-390.

Sun, D., Yu, Y., Xue, X., Pan, M., Wen, M., Li, S., ... & Tian, C. (2018). Cryo-EM structure of the ASIC1a–mambalgin-1 complex reveals that the peptide toxin mambalgin-1 inhibits acid-sensing ion channels through an unusual allosteric effect. Cell discovery, 4(1), 1-11.

Gregory, N. S., Gautam, M., Benson, C. J., & Sluka, K. A. (2018). Acid sensing ion channel 1a (ASIC1a) mediates activity-induced pain by modulation of heteromeric ASIC channel kinetics. Neuroscience, 386, 166-174.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-ASIC1 Recombinant Antibody (N271-44)

Isotype control

For research use only. Not intended for any clinical use.

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