Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-ATF6 Recombinant Antibody (3D5) (CBMAB-A0569-LY)


See all ATF6 antibodies
Online Inquiry

Summary

Host Animal
Mouse
Specificity
Human
Clone
3D5
Antibody Isotype
IgG2a, κ
Application
WB, ELISA, IHC-P

Basic Information

Immunogen
ATF6 (AAH14969.1, 91 a.a. ~ 193 a.a) partial recombinant protein with GST tag.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IHC-P3 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Activating Transcription Factor 6
Introduction
ATF6 is an endoplasmic reticulum (ER) stress-regulated transmembrane transcription factor that activates the transcription of ER molecules.[supplied by OMIM
Entrez Gene ID
22926
UniProt ID
P18850
Alternative Names
ATF6A
Function
Cyclic AMP-dependent transcription factor ATF-6 alpha: Precursor of the transcription factor form (Processed cyclic AMP-dependent transcription factor ATF-6 alpha), which is embedded in the endoplasmic reticulum membrane (PubMed:10564271, PubMed:11158310, PubMed:11779464).
Endoplasmic reticulum stress promotes processing of this form, releasing the transcription factor form that translocates into the nucleus, where it activates transcription of genes involved in the unfolded protein response (UPR) (PubMed:10564271, PubMed:11158310, PubMed:11779464).
Processed cyclic AMP-dependent transcription factor ATF-6 alpha: Transcription factor that initiates the unfolded protein response (UPR) during endoplasmic reticulum stress by activating transcription of genes involved in the UPR (PubMed:10564271, PubMed:11163209, PubMed:11158310, PubMed:11779464).
Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N9-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3') (PubMed:10564271, PubMed:11158310, PubMed:11779464).
Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor (PubMed:10564271, PubMed:11158310, PubMed:11779464).
May play a role in foveal development and cone function in the retina (PubMed:26029869).
Biological Process
ATF6-mediated unfolded protein response Source: ParkinsonsUK-UCL
Endoplasmic reticulum unfolded protein response Source: GO_Central
Eye development Source: UniProtKB
Positive regulation of apoptotic process Source: ParkinsonsUK-UCL
Positive regulation of ATF6-mediated unfolded protein response Source: UniProtKB
Positive regulation of autophagy Source: MGI
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress Source: ParkinsonsUK-UCL
Protein folding Source: ProtInc
Regulation of transcription by RNA polymerase II Source: GO_Central
Signal transduction Source: ProtInc
Visual perception Source: UniProtKB
Cellular Location
Golgi apparatus membrane; Endoplasmic reticulum membrane. Translocates from the endoplasmic reticulum to the Golgi, where it is processed.
Processed cyclic AMP-dependent transcription factor ATF-6 alpha: Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus (PubMed:10564271, PubMed:12782636). THBS4 promotes its nuclear shuttling (By similarity).
Involvement in disease
Achromatopsia 7 (ACHM7): A form of achromatopsia, an ocular stationary disorder due to the absence of functioning cone photoreceptors in the retina. It is characterized by total colorblindness, low visual acuity, photophobia and nystagmus.
Topology
Cytoplasmic: 1-377 aa
Helical: 378-398 aa
Lumenal: 399-670 aa
PTM
During unfolded protein response, a fragment of approximately 50 kDa containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 (MBTPS1, S1P) and site-2 (MBTPS2, S2P) proteases.
N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR).
More Infomation

Shen, Y., Li, R., Yu, S., Zhao, Q., Wang, Z., Sheng, H., & Yang, W. (2021). Activation of the ATF6 (Activating Transcription Factor 6) Signaling Pathway in Neurons Improves Outcome After Cardiac Arrest in Mice. Journal of the American Heart Association, e020216.

Ji, Z., Zhou, Z. L., Hao, Q., Zhao, L., Cui, C., Huang, S. B., ... & Shen, Y. Q. (2021). Activating Transcription Factor 6 Contributes to Functional Recovery After Spinal Cord Injury in Adult Zebrafish. Journal of Molecular Neuroscience, 71(4), 734-745.

Cheng, N., Shi, J. H., Jin, Y., Shi, Y. B., Liu, X. D., Zhang, H. P., ... & Zhang, S. J. (2021). Pharmacological Activating Transcription Factor 6 Activation Is Beneficial for Liver Retrieval With ex vivo Normothermic Mechanical Perfusion From Cardiac Dead Donor Rats. Frontiers in Surgery, 8, 212.

Tao, J., Chen, H., Li, X., & Wang, J. (2021). The role of activating transcription factor 6 in hydroxycamptothecin-induced fibroblast autophagy and apoptosis. Journal of Orthopaedic Surgery and Research, 16(1), 1-8.

Stengel, S. T., Fazio, A., Lipinski, S., Jahn, M. T., Aden, K., Ito, G., ... & Rosenstiel, P. (2020). Activating transcription factor 6 mediates inflammatory signals in intestinal epithelial cells upon endoplasmic reticulum stress. Gastroenterology, 159(4), 1357-1374.

Liu, F., Chang, L., & Hu, J. (2020). Activating transcription factor 6 regulated cell growth, migration and inhibiteds cell apoptosis and autophagy via MAPK pathway in cervical cancer. Journal of reproductive immunology, 139, 103120.

Du, Y., Liu, X., Zhu, X., Liu, Y., Wang, X., & Wu, X. (2020). Activating transcription factor 6 reduces Aβ1–42 and restores memory in Alzheimer’s disease model mice. International Journal of Neuroscience, 130(10), 1015-1023.

Shen, T., Li, Y., Chen, Z., Liang, S., Qiu, Y., Zhu, L., ... & Qiu, L. (2020). Activating transcription factor 6 (ATF6) negatively regulates Polo-like kinase 4 expression via recruiting C/EBPβ to the upstream-promoter during ER stress. Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms, 1863(2), 194488.

Liu, S., Wang, W., Ge, W., Lv, X., Han, Z., Li, Y., ... & Song, L. (2020). An activating transcription factor 6 beta (ATF6β) regulates apoptosis of hemocyte during immune response in Crassostrea gigas. Fish & shellfish immunology, 99, 442-451.

Jegal, K. H., Park, S. M., Cho, S. S., Byun, S. H., Ku, S. K., Kim, S. C., ... & Cho, I. J. (2017). Activating transcription factor 6-dependent sestrin 2 induction ameliorates ER stress-mediated liver injury. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1864(7), 1295-1307.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-ATF6 Recombinant Antibody (3D5)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry