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Mouse Anti-ATP6V1B2 Recombinant Antibody (1E11) (CBMAB-A4092-YC)

Provided herein is a Mouse monoclonal antibody against Human ATPase H+ Transporting V1 Subunit B2. The antibody can be used for immunoassay techniques, such as WB.
See all ATP6V1B2 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
1E11
Antibody Isotype
IgG2a
Application
WB, IHC

Basic Information

Immunogen
Full length human recombinant protein of human ATP6V1B2 produced in HEK293T cell.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:2,000
IHC1:150

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.3, 1% BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
Introduction
ATP6V1B2 is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, z
Entrez Gene ID
Human526
Mouse11966
Rat117596
UniProt ID
HumanP21281
MouseP62814
RatP62815
Alternative Names
ATPase H+ Transporting V1 Subunit B2; ATPase, H+ Transporting, Lysosomal 56/58kDa, V1 Subunit B2; Endomembrane Proton Pump 58 KDa Subunit; Vacuolar Proton Pump Subunit B 2; V-ATPase Subunit B 2; ATP6B2; VPP3; HO57; ATPase, H+ Transporting, Lysosomal (Vacu
Function
Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (By similarity).
V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (PubMed:32001091).
In renal intercalated cells, can partially compensate the lack of ATP6V1B1 and mediate secretion of protons (H+) into the urine under base-line conditions but not in conditions of acid load (By similarity).
Biological Process
ATP metabolic process Source: InterPro
Cellular response to amino acid starvation Source: Reactome
Insulin receptor signaling pathway Source: Reactome
Ion transmembrane transport Source: Reactome
Phagosome acidification Source: Reactome
Proton transmembrane transport Source: ProtInc
Regulation of macroautophagy Source: ParkinsonsUK-UCL
Transferrin transport Source: Reactome
Cellular Location
Apical cell membrane; Cytoplasm; Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Involvement in disease
Zimmermann-Laband syndrome 2 (ZLS2): A form of Zimmermann-Laband syndrome, a rare developmental disorder characterized by facial dysmorphism with bulbous nose and thick floppy ears, gingival enlargement, hypoplasia or aplasia of terminal phalanges and nails, hypertrichosis, joint hyperextensibility, and hepatosplenomegaly. Some patients manifest intellectual disability with or without epilepsy. ZLS2 inheritance is autosomal dominant.
Deafness, congenital, with onychodystrophy, autosomal dominant (DDOD): An autosomal dominant syndrome characterized mainly by congenital sensorineural hearing loss accompanied by dystrophic or absent nails. Coniform teeth, selective tooth agenesis, and hands and feet abnormalities are present in some patients.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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