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Mouse Anti-BCL2A1 Recombinant Antibody (CBYY-0398) (CBMAB-0400-YY)


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Published Data
Mouse Anti-BCL2A1 Recombinant Antibody (CBYY-0398) (CBMAB-0400-YY) in WB
(A) Western blot analysis of Bfl-1/A1 protein expression after transfection of RAW 264.7 cells with nanoplexes formulated using anti-Bfl-1/A1 siRNA (80 pmol) for 72 h; (B) Bfl-1/A1 gene silencing monitored using RT-PCR. Bfl-1/A1 mRNA levels were normalized to bactin mRNA expression. Red bars indicate treatment with anti-Bfl-1/A1 siRNA whereas black bars indicate treatment with scrambled siRNA. Endogenous Bfl1/A1 gene expression in RAW 264.7 cells was stimulated using lipopolysaccharide from E. coli for 24 h. Bfl-1/A1 expression was analyzed after treatment with naked siRNA and nanoplexes for 4 h, followed by incubation with normal growth medium for further 65 h, with intermittent replacement of medium every 24 h. ...View More
Dandekar, P., Jain, R., Keil, M., Loretz, B., Koch, M., Wenz, G., & Lehr, C. M. (2015). Enhanced uptake and siRNA-mediated knockdown of a biologically relevant gene using cyclodextrin polyrotaxane. Journal of Materials Chemistry B, 3(13), 2590-2598.

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYY-0398
Antibody Isotype
IgG1, κ
Application
WB, IP, ELISA, IHC-P, IF

Basic Information

Immunogen
Amino acids 1-175 representing full length A1 of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
C-Terminus

Target

Full Name
BCL2 Related Protein A1
Introduction
This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators that are involved in a wide variety of cellular activities such as embryonic development, homeostasis and tumorigenesis. The protein encoded by this gene is able to reduce the release of pro-apoptotic cytochrome c from mitochondria and block caspase activation. This gene is a direct transcription target of NF-kappa B in response to inflammatory mediators, and is up-regulated by different extracellular signals, such as granulocyte-macrophage colony-stimulating factor (GM-CSF), CD40, phorBovinel ester and inflammatory cytokine TNF and IL-1, which suggests a cytoprotective function that is essential for lymphocyte activation as well as cell survival. Alternatively spliced transcript variants encoding different isoforMouse have been found for this gene.
Entrez Gene ID
597
UniProt ID
Q16548
Alternative Names
BCL2 Related Protein A1; Hemopoietic-Specific Early Response Protein; Bcl-2-Like Protein 5; Protein BFL-1; Bcl2-L-5; BCL2L5; HBPA1; BFL1; GRS;
Function
Retards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection (By similarity).
Can inhibit apoptosis induced by serum starvation in the mammary epithelial cell line HC11 (By similarity).
Biological Process
Aging Source: Ensembl
Cerebral cortex development Source: Ensembl
Extrinsic apoptotic signaling pathway in absence of ligand Source: GO_Central
Intrinsic apoptotic signaling pathway in response to DNA damage Source: GO_Central
Negative regulation of apoptotic process Source: UniProtKB
Cellular Location
Cytoplasm
More Infomation

Chilakamarti, R. (2021). Lung Epithelial Regulation of BCL2 Related Protein A1 (BCL2A1) by Coronaviruses (SARS-CoV) and Type I Interferon Signaling. bioRxiv.

Liu, F., Pan, R., Ding, H., Gu, L., Yang, Y., Li, C., ... & Nie, Y. (2021). UBQLN4 is an ATM substrate that stabilizes the anti‐apoptotic proteins BCL2A1 and BCL2L10 in mesothelioma. Molecular Oncology.

Li, X., Dou, J., You, Q., & Jiang, Z. (2021). Inhibitors of BCL2A1/Bfl-1 Protein: Potential Stock in Cancer Therapy. European Journal of Medicinal Chemistry, 113539.

Zheng, Q., Gan, G., Gao, X., Luo, Q., & Chen, F. (2021). Targeting the IDO-BCL2A1-Cytochrome c Pathway Promotes Apoptosis in Oral Squamous Cell Carcinoma. OncoTargets and therapy, 14, 1673.

Lin, P., Li, J., Ye, F., Fu, W., Hu, X., Shao, Z., & Song, C. (2020). KCNN4 induces multiple chemoresistance in breast cancer by regulating BCL2A1. American journal of cancer research, 10(10), 3302.

Kim, J. T., Cho, H. J., Cho, M. Y., Lim, J., Park, E. S., Lim, J. S., & Lee, H. G. (2019). Prenylated Rab acceptor RABAC1 inhibits anti-apoptotic protein BCL2A1 and induces apoptosis. Biochemical and biophysical research communications, 513(4), 940-946.

Hiraki, M., Maeda, T., Mehrotra, N., Jin, C., Alam, M., Bouillez, A., ... & Kufe, D. (2018). Targeting MUC1-C suppresses BCL2A1 in triple-negative breast cancer. Signal transduction and targeted therapy, 3(1), 1-8.

Sochalska, M., Schuler, F., Weiss, J. G., Prchal-Murphy, M., Sexl, V., & Villunger, A. (2017). MYC selects against reduced BCL2A1/A1 protein expression during B cell lymphomagenesis. Oncogene, 36(15), 2066-2073.

Berberich, I., & Hildeman, D. A. (2017). The Bcl2a1 gene cluster finally knocked out: first clues to understanding the enigmatic role of the Bcl-2 protein A1. Cell death and differentiation, 24(4), 572.

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For research use only. Not intended for any clinical use.

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