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Rabbit Anti-CASP6 Recombinant Antibody (BA0016) (CBMAB-0163CQ)

This product is a rabbit antibody that recognizes CASP6. The antibody BA0016 can be used for immunoassay techniques such as: WB, IP, FC, IHC-P, IF.
See all CASP6 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
BA0016
Antibody Isotype
IgG
Application
WB, IP, FC, IHC-P, IF

Basic Information

Immunogen
Synthetic peptide from Human Caspase-6 aa 150-250
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Caspase 6
Introduction
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family of enzymes. Caspases (cysteinyl aspartate proteases) are involved in the signaling pathways of apoptosis, necrosis and inflammation. These enzymes can be divided into initiators and effectors. The initiator isoforms are activated by, and interact with, upstream adaptor molecules.
Entrez Gene ID
Human839
Mouse12368
Rat83584
UniProt ID
HumanP55212
MouseO08738
RatO35397
Alternative Names
Caspase 6, Apoptosis-Related Cysteine Peptidase; Caspase 6, Apoptosis-Related Cysteine Protease; EC 3.4.22.59; MCH2; Apoptotic Protease MCH-2; Apoptotic Protease Mch-2; EC 3.4.22; CASP-6
Function
Cysteine protease that plays essential roles in programmed cell death, axonal degeneration, development and innate immunity (PubMed:8663580, PubMed:32298652).
During apoptosis, localizes in the nucleus and cleaves the nuclear structural protein NUMA1 and lamin A/LMNA thereby inducing nuclear shrinkage and fragmentation (PubMed:17401638, PubMed:8663580, PubMed:9463409).
Furthermore, cleaves many transcription factors such as NF-kappa-B and cAMP response element-binding protein/CREBBP (PubMed:10559921, PubMed:14657026).
Plays an essential role in axon degeneration during axon pruning which is the remodeling of axons during neurogenesis but not apoptosis (By similarity).
Regulates B-cell programs both during early development and after antigen stimulation (By similarity).
In addition, promotes the ZBP1-mediated activation of programmed cell death pathways including pyroptosis, apoptosis, and necroptosis (PANoptosis) and plays an essential role in defense against viruses (PubMed:32298652).
Mechanistically, interacts with RIPK3 and enhances the interaction between RIPK3 and ZBP1, leading to ZBP1-mediated inflammasome activation and cell death (PubMed:32298652).
Biological Process
Apoptotic process Source: GO_Central
Cellular response to staurosporine Source: CAFA
Epithelial cell differentiation Source: UniProtKB
Execution phase of apoptosis Source: Reactome
Proteolysis Source: UniProtKB
Regulation of apoptotic process Source: Reactome
Cellular Location
Cytoplasm
PTM
Phosphorylated by NUAK1; phosphorylation inhibits self-activation.
Cleavages by caspase-8 and subsequently by caspase-3 generate the two active subunits.

Flores, J., Noël, A., Fillion, M. L., & LeBlanc, A. C. (2021). Therapeutic potential of Nlrp1 inflammasome, Caspase-1, or Caspase-6 against Alzheimer disease cognitive impairment. Cell Death & Differentiation, 1-13.

Zheng, M., Karki, R., Kancharana, B., Berns, H., Pruett-Miller, S. M., & Kanneganti, T. D. (2021). Caspase-6 promotes activation of the caspase-11-NLRP3 inflammasome during gram-negative bacterial infections. Journal of Biological Chemistry, 101379.

Noël, A., Foveau, B., & LeBlanc, A. C. (2021). Caspase-6-cleaved Tau fails to induce Tau hyperphosphorylation and aggregation, neurodegeneration, glial inflammation, and cognitive deficits. Cell death & disease, 12(3), 1-20.

Zhou, L., Flores, J., Noël, A., Beauchet, O., Sjöström, P. J., & LeBlanc, A. C. (2019). Methylene blue inhibits Caspase-6 activity, and reverses Caspase-6-induced cognitive impairment and neuroinflammation in aged mice. Acta neuropathologica communications, 7(1), 1-18.

Islam, M. I., Nagakannan, P., Ogungbola, O., Djordjevic, J., Albensi, B. C., & Eftekharpour, E. (2019). Thioredoxin system as a gatekeeper in caspase-6 activation and nuclear lamina integrity: Implications for Alzheimer's disease. Free Radical Biology and Medicine, 134, 567-580.

Tubeleviciute-Aydin, A., Zhou, L., Sharma, G., Soni, I. V., Savinov, S. N., Hardy, J. A., & LeBlanc, A. C. (2018). Rare human Caspase-6-R65W and Caspase-6-G66R variants identify a novel regulatory region of Caspase-6 activity. Scientific reports, 8(1), 1-14.

Girling, K. D., Demers, M. J., Laine, J., Zhang, S., Wang, Y. T., & Graham, R. K. (2018). Activation of caspase‐6 and cleavage of caspase‐6 substrates is an early event in NMDA receptor–mediated excitotoxicity. Journal of neuroscience research, 96(3), 391-406.

Offen, D., Angel, A., & Ben-Zur, T. (2018). Caspase-6 knock-out using CRISPR/Cas9 improves cognitive behavior in the 3xTg mouse model of Alzheimer's disease. Cytotherapy, 20(5), S94-S95.

Woo, V., Cheng, C., Duraikannu, A., Chandrasekhar, A., Purdy, K., Martinez, J. A., & Zochodne, D. W. (2018). Caspase-6 is a dispensable enabler of adult mammalian axonal degeneration. Neuroscience, 371, 242-253.

Skotte, N. H., Sanders, S. S., Singaraja, R. R., Ehrnhoefer, D. E., Vaid, K., Qiu, X., ... & Hayden, M. R. (2017). Palmitoylation of caspase-6 by HIP14 regulates its activation. Cell Death & Differentiation, 24(3), 433-444.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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