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Mouse Anti-CD180 Recombinant Antibody (CBT3081) (V2LY-0625-LY1889)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT3081
Antibody Isotype
IgG1
Application
IHC, ICC, FC

Basic Information

Immunogen
Purified recombinant fragment of human CD180 (AA: extra 24-185) expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
IHC-P1:200-1:1,000
ICC1:200-1:1,000
FC1:200-1:400
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
CD180 Molecule
Entrez Gene ID
4064
UniProt ID
Q99467
Function
May cooperate with MD-1 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) in B-cells. Leads to NF-kappa-B activation. Also involved in the life/death decision of B-cells (By similarity).
Biological Process
B cell proliferation involved in immune response Source: GO_Central
Cellular response to lipopolysaccharide Source: GO_Central
Inflammatory response Source: UniProtKB-KW
Innate immune response Source: UniProtKB-KW
Positive regulation of lipopolysaccharide-mediated signaling pathway Source: MGI
Cellular Location
Cell membrane
Topology
Extracellular: 24-626
Helical: 627-650
Cytoplasmic: 651-661
More Infomation

Hayden, Z., Erdő-Bonyár, S., Bóné, B., Balázs, N., Bodó, K., Illes, Z., ... & Simon, D. (2021). Toll-Like Receptor Homolog CD180 Expression Is Diminished on Natural Autoantibody-Producing B Cells of Patients with Autoimmune CNS Disorders. Journal of Immunology Research, 2021.

Miguet, L., Mayeur-Rousse, C., Eischen, A., Galoisy, A. C., Rolland, D., Gervais, C., ... & Mauvieux, L. (2021). The Contribution of CD148, CD180 and CD200 Combination in the Diagnosis of Chronic B-Cell Lymphoproliferative Disorders. Blood, 138(Supplement 1), 3520-3520.

Edwards, K., Volpi, E., Lydyard, P., Zaitseva, K., Sayed, U., Krysov, S., ... & Porakishvili, N. (2021). Expression patterns of CD180 in the lymph nodes of patients with chronic lymphocytic leukaemia. British Journal of Haematology, 195(2), e131-e134.

Dong, G., Yao, X., Yan, F., Zhang, H., Zhu, Y., Yang, Y., ... & Xiong, H. (2019). Ligation of CD180 contributes to endotoxic shock by regulating the accumulation and immunosuppressive activity of myeloid-derived suppressor cells through STAT3. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1865(3), 535-546.

Roe, K., Shu, G. L., Draves, K. E., Giordano, D., Pepper, M., & Clark, E. A. (2019). Targeting antigens to CD180 but not CD40 programs immature and mature B cell subsets to become efficient APCs. The Journal of Immunology, 203(7), 1715-1729.

Kikuchi, J., Kuroda, Y., Koyama, D., Osada, N., Izumi, T., Yasui, H., ... & Furukawa, Y. (2018). Myeloma cells are activated in bone marrow microenvironment by the CD180/MD-1 complex, which senses lipopolysaccharide. Cancer research, 78(7), 1766-1778.

Carreras-González, A., Navasa, N., Martín-Ruiz, I., Lavín, J. L., Azkargorta, M., Atondo, E., ... & Anguita, J. (2018). A multi-omic analysis reveals the regulatory role of CD180 during the response of macrophages to Borrelia burgdorferi. Emerging microbes & infections, 7(1), 1-13.

Yang, Y., Wang, C., Cheng, P., Zhang, X., Li, X., Hu, Y., ... & Xiong, H. (2018). CD180 Ligation inhibits TLR7-and TLR9-mediated activation of macrophages and dendritic cells through the Lyn-SHP-1/2 axis in murine lupus. Frontiers in immunology, 9, 2643.

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For research use only. Not intended for any clinical use.

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