Sign in or Register   Sign in or Register
  |  

Mouse Anti-CD244 Recombinant Antibody (10B1116) (CBMAB-C2102-LY)

This product is antibody recognizes CD244. The antibody 10B1116 immunoassay techniques such as: FC, IP.
See all CD244 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
10B1116
Antibody Isotype
IgG1
Application
FC, IP

Basic Information

Immunogen
CD244 Fusion protein
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
CD244 Molecule
Introduction
CD244 (CD244 Molecule) is a Protein Coding gene. Diseases associated with CD244 include Rheumatoid Arthritis and Lymphoproliferative Syndrome, X-Linked, 1. Among its related pathways are Response to elevated platelet cytosolic Ca2+ and Hematopoietic Stem Cell Differentiation Pathways and Lineage-specific Markers. Gene Ontology (GO) annotations related to this gene include receptor activity.
Entrez Gene ID
51744
UniProt ID
Q9BZW8
Alternative Names
CD244 Molecule; CD244 Molecule, Natural Killer Cell Receptor 2B4; Signaling Lymphocytic Activation Molecule 4; NK Cell Type I Receptor Protein 2B4; NK Cell Activation-Inducing Ligand; Natural Killer Cell Receptor 2B4; SLAM Family Member 4; NKR2B4; SLAMF4;
Function
Heterophilic receptor of the signaling lymphocytic activation molecule (SLAM) family; its ligand is CD48. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Acts as activating natural killer (NK) cell receptor (PubMed:10359122, PubMed:8376943, PubMed:11714776).
Activating function implicates association with SH2D1A and FYN (PubMed:15713798).
Downstreaming signaling involves predominantly VAV1, and, to a lesser degree, INPP5D/SHIP1 and CBL. Signal attenuation in the absence of SH2D1A is proposed to be dependent on INPP5D and to a lesser extent PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:10934222, PubMed:15713798).
Stimulates NK cell cytotoxicity, production of IFN-gamma and granule exocytosis (PubMed:8376943, PubMed:11714776).
Optimal expansion and activation of NK cells seems to be dependent on the engagement of CD244 with CD48 expressed on neighboring NK cells (By similarity).
Acts as costimulator in NK activation by enhancing signals by other NK receptors such as NCR3 and NCR1 (PubMed:10741393).
At early stages of NK cell differentiation may function as an inhibitory receptor possibly ensuring the self-tolerance of developing NK cells (PubMed:11917118).
Involved in the regulation of CD8+ T-cell proliferation; expression on activated T-cells and binding to CD488 provides costimulatory-like function for neighboring T-cells (By similarity).
Inhibits inflammatory responses in dendritic cells (DCs) (By similarity).
Biological Process
Adaptive immune response Source: UniProtKB-KW
Innate immune response Source: UniProtKB-KW
Leukocyte migration Source: Reactome
Natural killer cell activation involved in immune response Source: UniProtKB
Positive regulation of granzyme B production Source: UniProtKB
Positive regulation of inositol phosphate biosynthetic process Source: UniProtKB
Positive regulation of interferon-gamma production Source: UniProtKB
Positive regulation of interleukin-8 production Source: UniProtKB
Signal transduction Source: ProtInc
Cellular Location
Cell membrane; Membrane. Receptor engagement results in a recruitment to lipid drafts essential for the subsequent tyrosine phosphorylation of the ITSMs.
Topology
Extracellular: 22-229
Helical: 230-250
Cytoplasmic: 251-370
PTM
N-linked glycosylation is essential for the binding to its ligand CD48. Also O-glycosylated, in contrast, O-linked sialylation has a negative impact on ligand binding.
Phosphorylated by FYN and CSK on tyrosine residues following activation. Coligation with inhibitory receptors such as KIR2DL1 inhibits phosphorylation upon contact of NK cells with sensitive target cells.

Sun, L., Gang, X., Li, Z., Zhao, X., Zhou, T., Zhang, S., & Wang, G. (2021). Advances in Understanding the Roles of CD244 (SLAMF4) in Immune Regulation and Associated Diseases. Frontiers in Immunology, 12, 731.

Berger, A. E., Durrieu, C., Dzviga, C., Perrot, J. L., & Lambert, C. (2021). Human peripheral basophils extended phenotype shows a high expression of CD244 immuno-regulatory receptor. Journal of Immunological Methods, 492, 112951.

Agresta, L., Lehn, M., Lampe, K., Cantrell, R., Hennies, C., Szabo, S., ... & Janssen, E. M. (2020). CD244 represents a new therapeutic target in head and neck squamous cell carcinoma. Journal for immunotherapy of cancer, 8(1).

Buller, C. W., Mathew, P. A., & Mathew, S. O. (2020). Roles of NK cell receptors 2B4 (CD244), CS1 (CD319), and LLT1 (CLEC2D) in cancer. Cancers, 12(7), 1755.

Malaer, J. D., Marrufo, A. M., & Mathew, P. A. (2019). 2B4 (CD244, SLAMF4) and CS1 (CD319, SLAMF7) in systemic lupus erythematosus and cancer. Clinical Immunology, 204, 50-56.

Sigurdsson, V., Koide, S., Radulovic, V., Mansell, E., Van Der Garde, M., Örd, T., ... & Miharada, K. (2019). CD244 Marks Non-Functional Hematopoietic Stem Cells with a Mast Cell Signature after Induction of Endoplasmic Reticulum Stress. Blood, 134, 2474.

Agresta, L., Hoebe, K. H., & Janssen, E. M. (2018). The emerging role of CD244 signaling in immune cells of the tumor microenvironment. Frontiers in immunology, 9, 2809.

Mak, A., Thornhill, S. I., Lee, H. Y., Lee, B., Poidinger, M., Connolly, J. E., & Fairhurst, A. M. (2018). Brief report: Decreased expression of CD244 (SLAMF4) on monocytes and platelets in patients with systemic lupus erythematosus. Clinical rheumatology, 37(3), 811-816.

Ahmad, F., Shankar, E. M., Yong, Y. K., Tan, H. Y., Ahrenstorf, G., Jacobs, R., ... & Ansari, A. W. (2017). Negative checkpoint regulatory molecule 2B4 (CD244) upregulation is associated with invariant natural killer T cell alterations and human immunodeficiency virus disease progression. Frontiers in immunology, 8, 338.

Zhang, F., Liu, X., Chen, C., Zhu, J., Yu, Z., Xie, J., ... & Zheng, J. (2017). CD244 maintains the proliferation ability of leukemia initiating cells through SHP-2/p27kip1 signaling. Haematologica, 102(4), 707.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-CD244 Recombinant Antibody (10B1116)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare