Rabbit Anti-CENPI Recombinant Antibody (D4D5K) (CBMAB-C2240-CN)

This product is a Rabbit antibody that recognizes CENPI. The antibody D4D5K can be used for immunoassay techniques such as: WB.
See all CENPI antibodies

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human

Clone

D4D5K

Application

Basic Information

Immunogen

Synthetic peptide corresponding to residues surrounding Asn41 of human CENP-I

Specificity

Human

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

10 mM Sodium HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/mL BSA, 50% glycerol

Preservative

0.02% Sodium azide

Target

Full Name

Centromere Protein I

Introduction

This gene encodes a centromere protein that is a component of the CENPA-NAC (nucleosome-associated) complex. This complex is critical for accurate chromosome alignment and segregation and it ensures proper mitotic progression. This protein regulates the recruitment of kinetochore-associated proteins that are required to generate the spindle checkpoint signal. The product of this gene is involved in the response of gonadal tissues to follicle-stimulating hormone. Mutations in this gene may be involved in human X-linked disorders of gonadal development and gametogenesis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 13. [provided by RefSeq, Jan 2016]

Entrez Gene ID

2491

UniProt ID

Q92674

Alternative Names

Centromere Protein I; Follicle-Stimulating Hormone Primary Response Protein; Interphase Centromere Complex Protein 19; Leucine-Rich Primary Response Protein 1; FSHPRH1; CENP-I; LRPR1;

Function

Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Required for the localization of CENPF, MAD1L1 and MAD2 (MAD2L1 or MAD2L2) to kinetochores. Involved in the response of gonadal tissues to follicle-stimulating hormone.

Biological Process

CENP-A containing nucleosome assembly Source: Reactome
Mitotic spindle organization Source: Reactome
Sex differentiation Source: ProtInc

Cellular Location

Nucleus; Centromere. Localizes exclusively in the centromeres. The CENPA-CAD complex is probably recruited on centromeres by the CENPA-NAC complex.

PTM

Sumoylated. Sumoylated form can be polyubiquitinated by RNF4, leading to its degradation. Desumoylation by SENP6 prevents its degradation.

References

Wang, J., Liu, X., Chu, H. J., Li, N., Huang, L. Y., & Chen, J. (2021). Centromere Protein I (CENP-I) Is Upregulated in Gastric Cancer, Predicts Poor Prognosis, and Promotes Tumor Cell Proliferation and Migration. Technology in Cancer Research & Treatment, 20, 15330338211045510.

Basit, S., Al-Edressi, H. M., Sairafi, M. H., Hashmi, J. A., Alharby, E., Safar, R., & Ramzan, K. (2020). Centromere protein I (CENPI) is a candidate gene for X-linked steroid sensitive nephrotic syndrome. Journal of nephrology, 33(4), 763-769.

Ding, N., Li, R., Shi, W., & He, C. (2018). CENPI is overexpressed in colorectal cancer and regulates cell migration and invasion. Gene, 674, 80-86.

Thangavelu, P. U., Lin, C. Y., Vaidyanathan, S., Nguyen, T. H., Dray, E., & Duijf, P. H. (2017). Overexpression of the E2F target gene CENPI promotes chromosome instability and predicts poor prognosis in estrogen receptor-positive breast cancer. Oncotarget, 8(37), 62167.

Q & As

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Review & reward

Have you used Rabbit Anti-CENPI Recombinant Antibody (D4D5K)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote

Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email: