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Rat Anti-CENPT Recombinant Antibody (42F10) (CBMAB-C6478-LY)

This product is antibody recognizes CENPT. The antibody 42F10 immunoassay techniques such as: ICC, IP, WB.
See all CENPT antibodies

Summary

Host Animal
Rat
Specificity
Human
Clone
42F10
Antibody Isotype
IgG2a
Application
ICC, IP, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
50% glycerol
Concentration
1 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Centromere Protein T
Introduction
The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPT is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM, Mar 2008]
Entrez Gene ID
80152
UniProt ID
Q96BT3
Alternative Names
Centromere Protein T; Interphase Centromere Complex Protein 22; C16orf56; CENP-T; Chromosome 16 Open Reading Frame 56; ICEN22;
Function
Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPT has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis.
Biological Process
Cell division Source: UniProtKB-KW
CENP-A containing nucleosome assembly Source: Reactome
Chromosome organization Source: UniProtKB
Chromosome segregation Source: UniProtKB
Kinetochore assembly Source: UniProtKB
Mitotic cell cycle Source: UniProtKB
Mitotic spindle organization Source: Reactome
Protein localization to kinetochore involved in kinetochore assembly Source: GO_Central
Cellular Location
Nucleus; Centromere; Kinetochore. Constitutively localizes to centromeres throughout the cell cycle, and to kinetochores during mitosis. Localizes to the inner kinetochore, and may connect it to the outer kinetochore via its N-terminus.
Involvement in disease
Short stature and microcephaly with genital anomalies (SSMGA): An autosomal recessive disease characterized by growth failure resulting in severe short stature, severe microcephaly, and delayed and dissociated bone age. Additional features include global psychomotor developmental delay, pubertal delay and genital anomalies.
PTM
Dynamically phosphorylated at Ser-47 and probably also other sites during the cell cycle. Phosphorylated at Ser-47 during G2 phase, metaphase and anaphase, but not during telophase or G1 phase.

Dong, Q., Liu, X. L., Wang, X. H., Zhao, Y., Chen, Y. H., & Li, F. (2021). Ccp1-Ndc80 switch at the N terminus of CENP-T regulates kinetochore assembly. Proceedings of the National Academy of Sciences, 118(48).

Wang, Y., Li, J., Dong, F., Yue, W., Ouyang, Y. C., Wang, Z. B., ... & Sun, Q. Y. (2020). CENP-T regulates both the G2/M transition and anaphase entry by acting through CDH1 in meiotic oocytes. Journal of cell science, 133(3), jcs238105.

Hinshaw, S. M., & Harrison, S. C. (2020). The structural basis for kinetochore stabilization by Cnn1/CENP-T. Current Biology, 30(17), 3425-3431.

Cortes-Silva, N., Ulmer, J., Kiuchi, T., Hsieh, E., Cornilleau, G., Ladid, I., ... & Drinnenberg, I. A. (2020). CenH3-independent kinetochore assembly in Lepidoptera requires CCAN, including CENP-T. Current Biology, 30(4), 561-572.

Ding, M., Jiang, J., Yang, F., Zheng, F., Fang, J., Wang, Q., ... & Yao, X. (2019). Holliday junction recognition protein interacts with and specifies the centromeric assembly of CENP-T. Journal of Biological Chemistry, 294(3), 968-980.

Yang, Y., & Yu, H. (2018). CENP-T bears the load in mitosis. Nature cell biology, 20(12), 1335-1337.

Lang, J., Barber, A., & Biggins, S. (2018). An assay for de novo kinetochore assembly reveals a key role for the CENP-T pathway in budding yeast. Elife, 7, e37819.

Giunta, S., & Funabiki, H. (2017). Integrity of the human centromere DNA repeats is protected by CENP-A, CENP-C, and CENP-T. Proceedings of the National Academy of Sciences, 114(8), 1928-1933.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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