Mouse Anti-CERS1 Recombinant Antibody (CBYJL-082) (CBMAB-L0138-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to Ceramide Synthase 1 (CERS1). The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all CERS1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBYJL-082

Antibody Isotype

IgG2a, κ

Application

ELISA, WB

Basic Information

Immunogen

Partial recombinant corresponding to aa301-350 from human LASS1 (NP_067090) with GST tag. MW of the GST tag alone is 26kD.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.4

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 301-350

Target

Full Name

Ceramide Synthase 1

Introduction

CERS1 is a ceramide synthase enzyme catalyzing the synthesis of ceramide, the hydrophobic moiety of sphingolipids. CERS1 synthesizes 18-carbon (C18) ceramide in brain neurons. Elevated expression of this gene may be associated with increased longevity, while decreased expression of this gene may be associated with myoclonus epilepsy with dementia in human patients. CERS1 is transcribed from a monocistronic mRNA as well as a bicistronic mRNA, which also encodes growth differentiation factor 1.

Entrez Gene ID

10715

UniProt ID

P27544

Alternative Names

Ceramide Synthase 1; Longevity Assurance (LAG1; S. Cerevisiae) Homolog 1; Longevity Assurance Gene 1 Protein Homolog 1; Protein UOG-1; LASS1; LAG1; UOG1

Function

Ceramide synthase that catalyzes formation of ceramide from sphinganine and acyl-CoA substrates, with high selectivity toward stearoyl-CoA (octadecanoyl-CoA; C18:0-CoA).

Biological Process

Cellular response to dithiothreitol Source: UniProtKB
Cellular response to drug Source: UniProtKB
Cellular response to mycotoxin Source: UniProtKB
Cellular response to UV-A Source: UniProtKB
Ceramide biosynthetic process Source: UniProtKB
Negative regulation of telomerase activity Source: UniProtKB
Sphingolipid biosynthetic process Source: UniProtKB

Cellular Location

Endoplasmic reticulum membrane

Involvement in disease

Epilepsy, progressive myoclonic 8 (EPM8): A form of progressive myoclonic epilepsy, a clinically and genetically heterogeneous group of disorders defined by the combination of action and reflex myoclonus, other types of epileptic seizures, and progressive neurodegeneration and neurocognitive impairment. EPM8 is an autosomal recessive form characterized by myoclonus, generalized tonic-clonic seizures and moderate to severe cognitive impairment.

PTM

Acetylated. Deacetylation by SIRT3 increases enzyme activity and promotes mitochondrial ceramide accumulation.

References

Chen, W., Wu, C., Chen, Y., Guo, Y., Qiu, L., Liu, Z., ... & Li, L. (2021). Downregulation of ceramide synthase 1 promotes oral cancer through endoplasmic reticulum stress. International journal of oral science, 13(1), 1-9.

Tosetti, B., Brodesser, S., Brunn, A., Deckert, M., Blüher, M., Doehner, W., ... & Krönke, M. (2020). A tissue‐specific screen of ceramide expression in aged mice identifies ceramide synthase‐1 and ceramide synthase‐5 as potential regulators of fiber size and strength in skeletal muscle. Aging cell, 19(1), e13049.

Turpin-Nolan, S. M., Hammerschmidt, P., Chen, W., Jais, A., Timper, K., Awazawa, M., ... & Brüning, J. C. (2019). CerS1-derived C18: 0 ceramide in skeletal muscle promotes obesity-induced insulin resistance. Cell Reports, 26(1), 1-10.

Turner, N., Lim, X. Y., Toop, H. D., Osborne, B., Brandon, A. E., Taylor, E. N., ... & Don, A. S. (2018). A selective inhibitor of ceramide synthase 1 reveals a novel role in fat metabolism. Nature communications, 9(1), 1-14.

Wang, Z., Wen, L., Zhu, F., Wang, Y., Xie, Q., Chen, Z., & Li, Y. (2017). Overexpression of ceramide synthase 1 increases C18-ceramide and leads to lethal autophagy in human glioma. Oncotarget, 8(61), 104022.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-CERS1 Recombinant Antibody (2E8)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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