Rabbit Anti-CLCN3 Recombinant Antibody (CBWJC-2758) (CBMAB-C3871WJ)

This product is a Rabbit antibody that recognizes CLCN3. This antibody CBWJC-2758 can be used for immunoassay techniques such as: WB, IP, IF.
See all CLCN3 antibodies

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human, Mouse, Rat

Clone

CBWJC-2758

Antibody Isotype

IgG

Application

WB, IP, IF

Basic Information

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Chloride Voltage-Gated Channel 3

Introduction

This gene encodes a member of the voltage-gated chloride channel (ClC) family. The encoded protein is present in all cell types and localized in plasma membranes and in intracellular vesicles. It is a multi-pass membrane protein which contains a ClC domain and two additional C-terminal CBS (cystathionine beta-synthase) domains. The ClC domain catalyzes the selective flow of Cl- ions across cell membranes, and the CBS domain may have a regulatory function. This protein plays a role in both acidification and transmitter loading of GABAergic synaptic vesicles, and in smooth muscle cell activation and neointima formation. This protein is required for lysophosphatidic acid (LPA)-activated Cl- current activity and fibroblast-to-myofibroblast differentiation. The protein activity is regulated by Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) in glioma cells. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Entrez Gene ID

Human	1182
Mouse	12725
Rat	84360

UniProt ID

Human	P51790
Mouse	P51791
Rat	P51792

Alternative Names

Chloride Voltage-Gated Channel 3; Chloride Channel, Voltage-Sensitive 3; Chloride Channel Protein 3; Chloride Transporter ClC-3; Chloride Channel 3; ClC-3; H(+)/Cl(-) Exchange Transporter 3; CLC3;

Function

Isoform 1:
Strongly outwardly rectifying, electrogenic H+/Cl-exchanger which mediates the exchange of chloride ions against protons (By similarity).

The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons (PubMed:29845874).

The presence of conserved gating glutamate residues is typical for family members that function as antiporters (PubMed:29845874).

Isoform 2:
Strongly outwardly rectifying, electrogenic H+/Cl-exchanger which mediates the exchange of chloride ions against protons.

Biological Process

Adult locomotory behavior Source: Ensembl
Chloride transmembrane transport Source: GO_Central
Endosomal lumen acidification Source: UniProtKB
Negative regulation of cell volume Source: GO_Central
Phagocytosis, engulfment Source: Ensembl
Photoreceptor cell maintenance Source: Ensembl
Positive regulation of reactive oxygen species biosynthetic process Source: Ensembl
Regulation of pH Source: UniProtKB
Synaptic transmission, GABAergic Source: Ensembl
Synaptic transmission, glutamatergic Source: Ensembl
Synaptic vesicle lumen acidification Source: Ensembl

Cellular Location

Isoform 1: Early endosome membrane; Late endosome membrane; Cell membrane; Lysosome membrane. Isoform 1 is localized mainly in late endosomes.
Isoform 2: Ruffle membrane; Golgi apparatus membrane. Isoform 2 is mainly enriched in the Golgi (PubMed:12471024). Colocalizes with SLC9A3R1/EBP50 in membrane ruffles (PubMed:11967229).

Topology

Cytoplasmic: 1-125
Helical: 126-163
Helical: 209-232
Helical: 241-248
Helical: 258-276
Helical: 282-301
Helical: 313-325
Helical: 329-337
Helical: 349-367
Helical: 391-416
Helical: 423-443
Helical: 500-520
Helical: 525-544
Helical: 572-586
Helical: 590-601
Loop between two helices: 602-605
Helical: 606-624
Cytoplasmic: 625-818

PTM

N-glycosylated.

References

Chen, Q., Liu, X., Luo, Z., Wang, S., Lin, J., Xie, Z., ... & Xu, B. (2019). Chloride channel‐3 mediates multidrug resistance of cancer by upregulating P‐glycoprotein expression. Journal of cellular physiology, 234(5), 6611-6623.

Takeno, T., Hasegawa, T., Hasegawa, H., Ueno, Y., Hamataka, R., Nakajima, A., ... & Sakamaki, T. (2019). MicroRNA-205-5p inhibits three-dimensional spheroid proliferation of ErbB2-overexpressing breast epithelial cells through direct targeting of CLCN3. PeerJ, 7, e7799.

Zhang, Y., Zhou, L. E. I., Zhang, J., Zhang, L., Yan, X., & Su, J. (2018). Suppression of chloride voltage‑gated channel 3 expression increases sensitivity of human glioma U251 cells to cisplatin through lysosomal dysfunction. Oncology letters, 16(1), 835-842.

Qi, Y., Mair, N., Kummer, K. K., Leitner, M. G., Camprubí-Robles, M., Langeslag, M., & Kress, M. (2018). Identification of chloride channels CLCN3 and CLCN5 mediating the excitatory Cl− currents activated by sphingosine-1-phosphate in sensory neurons. Frontiers in molecular neuroscience, 11, 33.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-CLCN3 Recombinant Antibody (S258-5)

Mouse Anti-CLCN3 Recombinant Antibody (CBLNC-161)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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