Sign in or Register   Sign in or Register
  |  

Mouse Anti-CLP1 Recombinant Antibody (CBYY-C3236) (CBMAB-C4679-YY)

This product is mouse antibody that recognizes CLP1. The antibody CBYY-C3236 can be used for immunoassay techniques such as: ELISA, WB
See all CLP1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYY-C3236
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
Partial recombinant corresponding to aa316-426 from human HEAB (NP_006822) with GST tag. MW of the GST tag alone is 26kD
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Protein A purified
Preservative
PBS, pH 7.2
Concentration
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cleavage And Polyadenylation Factor I Subunit 1
Entrez Gene ID
10978
UniProt ID
Q92989
Alternative Names
C4B112, C4B2, C4B3, C4B5, C4BD, C4B_2, C4F, CH, CO4, CPAMD3, C4B
Function
Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA (PubMed:24766809, PubMed:24766810).

Its role in tRNA splicing and maturation is required for cerebellar development (PubMed:24766809, PubMed:24766810).

Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.
Biological Process
Cerebellar cortex development Source: UniProtKB
mRNA 3'-end processing Source: UniProtKB
mRNA polyadenylation Source: GO_Central
mRNA splicing, via spliceosome Source: Reactome
RNA processing Source: GO_Central
siRNA loading onto RISC involved in RNA interference Source: UniProtKB
Targeting of mRNA for destruction involved in RNA interference Source: UniProtKB
Termination of RNA polymerase II transcription Source: Reactome
tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB
Cellular Location
Nucleus
Involvement in disease
Pontocerebellar hypoplasia 10 (PCH10):
The disease is caused by variants affecting the gene represented in this entry. Neurodegeneration is due to defects in tRNA splicing (PubMed:24766809, PubMed:24766810). A form of pontocerebellar hypoplasia, a disorder characterized by structural defects of the pons and cerebellum, evident upon brain imaging. PCH10 features include cortical dysgenesis marked by a simplified gyral pattern, cortical atrophy, mild or focal cerebellar vermian volume loss, delayed myelination, progressive microcephaly, global growth and developmental delays, severe intellectual disabilities, and seizures refractory to treatment.
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-CLP1 Recombinant Antibody (CBYY-C3236)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare