Sign in or Register   Sign in or Register
  |  

Mouse Anti-CPEB3 Antibody (1C1) (CBMAB-0235-YC)

Provided herein are mouse monoclonal antibodies against Human CPEB3. The antibody clone 1C1 can be used for immunoassay techniques, such as IP, WB and MA.
See all CPEB3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1C1
Antibody Isotype
IgG2b
Application
IP, WB, MA

Basic Information

Immunogen
Recombinant protein
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CPEB3
Introduction
CPEB is a highly conserved RNA-binding protein that promotes the elongation of the polyadenine tail of messenger RNA. CPEB most commonly activates the target RNA for translation, but can also act as a repressor, dependent on its phosphorylation state. In animals, CPEB is expressed in several alternative splicing isoforms that are specific to particular tissues and functions, including the self-cleaving Mammalian CPEB3 ribozyme.
Entrez Gene ID
22849
UniProt ID
Q8NE35
Alternative Names
KIAA0940
Function
Sequence-specific RNA-binding protein which acts as a translational repressor in the basal unstimulated state but, following neuronal stimulation, acts as a translational activator (By similarity).

In contrast to CPEB1, does not bind to the cytoplasmic polyadenylation element (CPE), a uridine-rich sequence element within the mRNA 3'-UTR, but binds to a U-rich loop within a stem-loop structure (By similarity).

Required for the consolidation and maintenance of hippocampal-based long term memory (By similarity).

In the basal state, binds to the mRNA 3'-UTR of the glutamate receptors GRIA2/GLUR2 mRNA and negatively regulates their translation (By similarity).

Also represses the translation of DLG4, GRIN1, GRIN2A and GRIN2B (By similarity).

When activated, acts as a translational activator of GRIA1 and GRIA2 (By similarity).

In the basal state, suppresses SUMO2 translation but activates it following neuronal stimulation (By similarity).

Binds to the 3'-UTR of TRPV1 mRNA and represses TRPV1 translation which is required to maintain normal thermoception (By similarity).

Binds actin mRNA, leading to actin translational repression in the basal state and to translational activation following neuronal stimulation (By similarity).

Negatively regulates target mRNA levels by binding to TOB1 which recruits CNOT7/CAF1 to a ternary complex and this leads to target mRNA deadenylation and decay (PubMed:21336257).

In addition to its role in translation, binds to and inhibits the transcriptional activation activity of STAT5B without affecting its dimerization or DNA-binding activity. This, in turn, represses transcription of the STAT5B target gene EGFR which has been shown to play a role in enhancing learning and memory performance (PubMed:20639532).

In contrast to CPEB1, CPEB2 and CPEB4, not required for cell cycle progression (PubMed:26398195).
Biological Process
3'-UTR-mediated mRNA destabilization Source: UniProtKB
Cellular response to amino acid stimulus Source: UniProtKB
Long-term memory Source: UniProtKB
Negative regulation of cytoplasmic translation Source: GO_Central
Negative regulation of cytoplasmic translational elongation Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Negative regulation of translation Source: UniProtKB
Positive regulation of dendritic spine development Source: UniProtKB
Positive regulation of mRNA polyadenylation Source: UniProtKB
Positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: UniProtKB
Positive regulation of nuclear-transcribed mRNA poly(A) tail shortening Source: UniProtKB
Positive regulation of translation Source: UniProtKB
Regulation of dendritic spine development Source: UniProtKB
Regulation of synaptic plasticity Source: UniProtKB
Cellular Location
Cytoplasm; Nucleus; Synapse; Dendrite; Postsynaptic density. Predominantly cytoplasmic in unstimulated neurons but translocates to the nucleus following neuronal stimulation (PubMed:20639532, PubMed:22730302). Nuclear import is mediated by importin IPO5 (By similarity).
PTM
Activated by NEURL1-mediated monoubiquitination, resulting in the growth of new dendritic spines and increased levels of GRIA1 and GRIA2. NEURL1-mediated monoubiquitination facilitates synaptic plasticity and hippocampal-dependent memory storage.
Under basal unstimulated conditions when CPEB3 is mainly unaggregated, sumoylated and acts as a translational repressor. Following neuronal stimulation, becomes desumoylated and aggregated which is required for the translation of mRNA targets and for dendritic filopodia formation.
Following neuronal stimulation, cleaved by CAPN2 which abolishes its translational repressor activity, leading to translation of CPEB3 target mRNAs.
Phosphorylation is enhanced by neuronal stimulation.

Zhang, H., Yin, W., Wang, C., Liu, Y., Li, Y., Wang, L., ... & Gao, X. (2021). CPEB3 deficiency in mice affect ovarian follicle development and causes premature ovarian insufficiency. Cell death & disease, 13(1), 1-9.

de Mingo, D. R., López-García, P., Hervás, R., Laurents, D. V., & Carrión-Vázquez, M. (2020). Molecular determinants of liquid demixing and amyloidogenesis in human CPEB3. BioRxiv.

Miao, K., Liu, S. D., Huang, W. X., & Dong, H. (2020). MiR-224 executes a tumor accelerative role during hepatocellular carcinoma malignancy by targeting cytoplasmic polyadenylation element-binding protein 3. Pharmacology, 105(7-8), 477-487.

Ford, L., Asok, A., Tripp, A. D., Parro, C., Fitzpatrick, M., de Solis, C. A., ... & Kandel, E. R. (2020). The low complexity motif of cytoplasmic polyadenylation element binding protein 3 (CPEB3) is critical for the trafficking of its targets in neurons. bioRxiv.

Zhang, H., Zou, C., Qiu, Z., Li, Q., Chen, M., Wang, D., ... & Gao, X. (2020). CPEB3-mediated MTDH mRNA translational suppression restrains hepatocellular carcinoma progression. Cell death & disease, 11(9), 1-17.

Qu, W. R., Sun, Q. H., Liu, Q. Q., Jin, H. J., Cui, R. J., Yang, W., ... & Li, B. J. (2020). Role of CPEB3 protein in learning and memory: new insights from synaptic plasticity. Aging (Albany NY), 12(14), 15169.

Ford, L., Ling, E., Kandel, E. R., & Fioriti, L. (2019). CPEB3 inhibits translation of mRNA targets by localizing them to P bodies. Proceedings of the National Academy of Sciences, 116(36), 18078-18087.

Liu, F., Zhang, G., Lv, S., Wen, X., & Liu, P. (2019). miRNA‐301b‐3p accelerates migration and invasion of high‐grade ovarian serous tumor via targeting CPEB3/EGFR axis. Journal of Cellular Biochemistry, 120(8), 12618-12627.

Lin, H., Guo, Q., Lu, S., Chen, J., Li, X., Gong, M., ... & Wen, J. (2019). LncRNA SUMO1P3 promotes proliferation and inhibits apoptosis in colorectal cancer by epigenetically silencing CPEB3. Biochemical and biophysical research communications, 511(2), 239-245.

Liu, H., Wang, Y., Chen, B., Shen, X., & Li, W. (2018). Effects of lidocaine-mediated CPEB3 upregulation in human hepatocellular carcinoma cell proliferation in vitro. BioMed Research International, 2018.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-CPEB3 Antibody (1C1)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare