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Mouse Anti-CR1 Recombinant Antibody (CBFYC-2173) (CBMAB-C2242-FY)

This product is mouse antibody that recognizes CR1. The antibody CBFYC-2173 can be used for immunoassay techniques such as: FC, IF, IHC.
See all CR1 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
CBFYC-2173
Antibody Isotype
IgG1
Application
FC, IF, IHC

Basic Information

Immunogen
Intact human monocytes. Epitope: Distal from C3b-binding site
Specificity
Human, Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Complement C3b/C4b Receptor 1 (Knops Blood Group)
Introduction
This gene is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in its gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus and sarcoidosis. Mutations in this gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. Alternate allele-specific splice variants, encoding different isoforms, have been characterized. Additional allele specific isoforms, including a secreted form, have been described but have not been fully characterized.
Entrez Gene ID
1378
UniProt ID
P17927
Alternative Names
KN; C3BR; C4BR; CD35
Function
Membrane immune adherence receptor that plays a critical role in the capture and clearance of complement-opsonized pathogens by erythrocytes and monocytes/macrophages (PubMed:2963069).

Mediates the binding by these cells of particles and immune complexes that have activated complement to eliminate them from the circulation (PubMed:2963069).

Acts also in the inhibition of spontaneous complement activation by impairing the formation and function of the alternative and classical pathway C3/C5 convertases, and by serving as a cofactor for the cleavage by factor I of C3b to iC3b, C3c and C3d,g, and of C4b to C4c and C4d (PubMed:2972794, PubMed:8175757).

Plays also a role in immune regulation by contributing, upon ligand binding, to the generation of regulatory T cells from activated helper T cells (PubMed:25742728).

(Microbial infection) Acts as a receptor for Epstein-Barr virus.
Biological Process
ATP export Source: ARUK-UCL
Complement activation, alternative pathway Source: ARUK-UCL
Complement activation, classical pathway Source: UniProtKB-KW
Complement receptor mediated signaling pathway Source: ARUK-UCL
Immune complex clearance by erythrocytes Source: ARUK-UCL
Negative regulation of activation of membrane attack complex Source: ARUK-UCL
Negative regulation of complement activation Source: ARUK-UCL
Negative regulation of complement activation, alternative pathway Source: UniProtKB
Negative regulation of complement activation, classical pathway Source: UniProtKB
Negative regulation of cytolysis Source: ARUK-UCL
Negative regulation of immunoglobulin production Source: ARUK-UCL
Negative regulation of interferon-gamma production Source: ARUK-UCL
Negative regulation of interleukin-2 production Source: ARUK-UCL
Negative regulation of plasma cell differentiation Source: ARUK-UCL
Negative regulation of serine-type endopeptidase activity Source: UniProtKB
Negative regulation of T cell proliferation Source: ARUK-UCL
Neutrophil degranulation Source: Reactome
Plasma membrane organization Source: ARUK-UCL
Positive regulation of activation of membrane attack complex Source: ARUK-UCL
Positive regulation of cell population proliferation Source: ARUK-UCL
Positive regulation of regulatory T cell differentiation Source: ARUK-UCL
Positive regulation of serine-type endopeptidase activity Source: UniProtKB
Regulation of complement activation Source: Reactome
Regulation of regulatory T cell differentiation Source: Reactome
Cellular Location
Membrane
Topology
Extracellular: 42-1971
Helical: 1972-1996
Cytoplasmic: 1997-2039

Kisserli, A., Schneider, N., Audonnet, S., Tabary, T., Goury, A., Cousson, J., ... & Cohen, J. H. (2021). Acquired decrease of the C3b/C4b receptor (CR1, CD35) and increased C4d deposits on erythrocytes from ICU COVID-19 patients. Immunobiology, 226(3), 152093.

Wymann, S., Dai, Y., Nair, A. G., Cao, H., Powers, G. A., Schnell, A., ... & Hardy, M. P. (2021). A novel soluble complement receptor 1 fragment with enhanced therapeutic potential. Journal of Biological Chemistry, 296.

Qidwai, T. (2021). Human Complement Receptor 1 Polymorphisms. In Exploration of Host Genetic Factors associated with Malaria (pp. 113-122). Springer, Singapore.

Simmons, K. T., Mazzilli, J. L., Mueller-Ortiz, S. L., Domozhirov, A. Y., Garcia, C. A., Zsigmond, E. M., & Wetsel, R. A. (2020). Complement Receptor 1 (CR1/CD35)-expressing retinal pigment epithelial cells as a potential therapy for age-related macular degeneration. Molecular Immunology, 118, 91-98.

Kisserli, A., Audonnet, S., Duret, V., Tabary, T., Cohen, J. H. M., & Mahmoudi, R. (2020). Measuring erythrocyte complement receptor 1 using flow cytometry. JoVE (Journal of Visualized Experiments), (159), e60810.

Kucukkilic, E., Brookes, K., Barber, I., Guetta-Baranes, T., Morgan, K., & Hollox, E. J. (2018). Complement receptor 1 gene (CR1) intragenic duplication and risk of Alzheimer’s disease. Human genetics, 137(4), 305-314.

Kretzschmar, G. C., Oliveira, L. C., Nisihara, R. M., Velavan, T. P., Stinghen, S. T., Stahlke, E. R., ... & Boldt, A. B. W. (2018). Complement receptor 1 (CR1, CD35) association with susceptibility to leprosy. PLoS neglected tropical diseases, 12(8), e0006705.

Mahmoudi, R., Feldman, S., Kisserli, A., Duret, V., Tabary, T., Bertholon, L. A., ... & Cohen, J. H. M. (2018). Inherited and acquired decrease in complement receptor 1 (CR1) density on red blood cells associated with high levels of soluble CR1 in Alzheimer’s disease. International Journal of Molecular Sciences, 19(8), 2175.

Jacquet, M., Cioci, G., Fouet, G., Bally, I., Thielens, N. M., Gaboriaud, C., & Rossi, V. (2018). C1q and mannose-binding lectin interact with CR1 in the same region on CCP24-25 modules. Frontiers in Immunology, 9, 453.

Kisserli, A., Tabary, T., Cohen, J. H. M., Duret, V., & Mahmoudi, R. (2017). High-resolution melting PCR for complement receptor 1 length polymorphism genotyping: an innovative tool for Alzheimer's disease gene susceptibility assessment. JoVE (Journal of Visualized Experiments), (125), e56012.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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