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Mouse Anti-CREB3L2 (AA 421-518) Recombinant Antibody (CBFYC-2196) (CBMAB-C2266-FY)

This product is mouse antibody that recognizes CREB3L2. The antibody CBFYC-2196 can be used for immunoassay techniques such as: ELISA, WB.
See all CREB3L2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYC-2196
Antibody Isotype
IgG2a, k
Application
ELISA, WB

Basic Information

Immunogen
CREB3L2 (NP_919047.2, 421 a.a.-518 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Immunogen sequence: ASVVRSRNLL IYEEHSPPEE SSSPGSAGEL GGWDRGSSLL RVSGLESRPD VDLPHFIISN ETSLEKSVLL ELQQHLVSAK LEGNETLKVV ELDRRVNT
Specificity
Human
Antibody Isotype
IgG2a, k
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 421-518

Target

Full Name
CAMP Responsive Element Binding Protein 3 Like 2
Introduction
CREB3L2 (CAMP Responsive Element Binding Protein 3 Like 2) is a Protein Coding gene. Diseases associated with CREB3L2 include Myxofibrosarcoma and Sarcoma. Among its related pathways are Aldosterone synthesis and secretion and Circadian entrainment. Gene Ontology (GO) annotations related to this gene include DNA binding transcription factor activity and RNA polymerase II proximal promoter sequence-specific DNA binding. An important paralog of this gene is CREB3L1.
Entrez Gene ID
64764
UniProt ID
Q70SY1
Alternative Names
CAMP Responsive Element Binding Protein 3 Like 2; CAMP-Responsive Element-Binding Protein 3-Like Protein 2; BBF2 Human Homolog On Chromosome 7; BBF2H7; Cyclic AMP-Responsive Element-Binding Protein 3-Like Protein 2; Basic Transcription Factor 2; B-ZIB Transcription Factor; FUS/BBF2H7 Protein; TCAG_1951439
Function
Transcription factor involved in unfolded protein response (UPR). In the absence of endoplasmic reticulum (ER) stress, inserted into ER membranes, with N-terminal DNA-binding and transcription activation domains oriented toward the cytosolic face of the membrane. In response to ER stress, transported to the Golgi, where it is cleaved in a site-specific manner by resident proteases S1P/MBTPS1 and S2P/MBTPS2. The released N-terminal cytosolic domain is translocated to the nucleus to effect transcription of specific target genes. Plays a critical role in chondrogenesis by activating the transcription of SEC23A, which promotes the transport and secretion of cartilage matrix proteins, and possibly that of ER biogenesis-related genes (By similarity).

In a neuroblastoma cell line, protects cells from ER stress-induced death (PubMed:17178827).

In vitro activates transcription of target genes via direct binding to the CRE site (PubMed:17178827).
Biological Process
Cartilage development Source: UniProtKB
Chondrocyte differentiation Source: UniProtKB
Endoplasmic reticulum to Golgi vesicle-mediated transport Source: Ensembl
Endoplasmic reticulum unfolded protein response Source: InterPro
Positive regulation of gene expression Source: Ensembl
Positive regulation of neuron projection development Source: Ensembl
Positive regulation of transcription, DNA-templated Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Response to endoplasmic reticulum stress Source: UniProtKB
Response to wounding Source: Ensembl
Cellular Location
Endoplasmic reticulum membrane. ER membrane resident protein. Upon ER stress, translocated to the Golgi apparatus where it is cleaved. The cytosolic N-terminal fragment (processed cyclic AMP-responsive element-binding protein 3-like protein 1) is transported into the nucleus.
Processed cyclic AMP-responsive element-binding protein 3-like protein 2: Nucleus. Upon ER stress, translocated into the nucleus.
Involvement in disease
A chromosomal aberration involving CREB3L2 is found in low grade fibromyxoid sarcoma (LGFMS). Translocation t(7;16)(q33;p11) with FUS.
Topology
Cytoplasmic: 1-379
Helical: 380-400
Lumenal: 401-520
PTM
Upon ER stress, translocated to the Golgi apparatus, where it is processed by regulated intramembrane proteolysis (RIP) to release the cytosol-facing N-terminal transcription factor domain. The cleavage is performed sequentially by site-1 and site-2 proteases (S1P/MBTPS1 and S2P/MBTPS2).
N-glycosylated.
Ubiquitinated by HRD1/SYVN1; undergoes 'Lys-48'-linked ubiquitination, followed by rapid proteasomal degradation under normal conditions. Upon ER stress, SYVN1 E3 ubiquitin-protein ligase dissociates from its substrate, ubiquitination does not occur and CREB3L2 is stabilized.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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