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Mouse Anti-CRISPR-Cas9 Recombinant Antibody (7A9) (CBMAB-V208-0928-CQ)

This product is a mouse antibody that recognizes CRISPR-Cas9. The antibody 7A9 can be used for immunoassay techniques such as: IF, IP, WB.
See all CRISPR-Cas9 antibodies
Published Data
Fig.1 Western blot analysis of Cas9 presence as a function of time.
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Summary

Host Animal
Mouse
Specificity
S. pyogenes
Clone
7A9
Antibody Isotype
IgG1, κ
Application
IF, IP, WB

Basic Information

Immunogen
Recombinant S. pyogenes Cas9 Protein (N-terminal 250 amino acids) expressed in E. coli
Specificity
S. pyogenes
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CRISPR-associated endonuclease Cas9
Introduction
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.
Alternative Names
Cas9; CRISPR-associated endonuclease Cas9/Csn1; CRISPR-Cas9/Csn1; csn1; SpyCas9; CRISPR-Cas9
Function
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids) (PubMed:21455174).

CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA; Cas9 only stabilizes the pre-crRNA:tracrRNA interaction and has no catalytic function in RNA processing (PubMed:24270795).

Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed 3'-5' exonucleolytically. DNA-binding requires protein and both gRNAs, as does nuclease activity. Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. DNA strand separation and heteroduplex formation starts at PAM sites; PAM recognition is required for catalytic activity (PubMed:24476820).

Confers immunity against a plasmid with homology to the appropriate CRISPR spacer sequences (CRISPR interference) (PubMed:21455174).
Biological Process
Defense response to virus Source: UniProtKB-UniRule
Maintenance of CRISPR repeat elements Source: UniProtKB

Yip, B. H. (2020). Recent advances in CRISPR/Cas9 delivery strategies. Biomolecules, 10(6), 839.

Zhan, T., Rindtorff, N., Betge, J., Ebert, M. P., & Boutros, M. (2019, April). CRISPR/Cas9 for cancer research and therapy. In Seminars in cancer biology (Vol. 55, pp. 106-119). Academic Press.

You, L., Tong, R., Li, M., Liu, Y., Xue, J., & Lu, Y. (2019). Advancements and obstacles of CRISPR-Cas9 technology in translational research. Molecular Therapy-Methods & Clinical Development, 13, 359-370.

Cui, Y., Xu, J., Cheng, M., Liao, X., & Peng, S. (2018). Review of CRISPR/Cas9 sgRNA design tools. Interdisciplinary Sciences: Computational Life Sciences, 10(2), 455-465.

Harrington, L. B., Doxzen, K. W., Ma, E., Liu, J. J., Knott, G. J., Edraki, A., ... & Doudna, J. A. (2017). A broad-spectrum inhibitor of CRISPR-Cas9. Cell, 170(6), 1224-1233.

Jiang, F., & Doudna, J. A. (2017). CRISPR–Cas9 structures and mechanisms. Annual review of biophysics, 46, 505-529.

Liu, X., Wu, S., Xu, J., Sui, C., & Wei, J. (2017). Application of CRISPR/Cas9 in plant biology. Acta pharmaceutica sinica B, 7(3), 292-302.

Pulecio, J., Verma, N., Mejía-Ramírez, E., Huangfu, D., & Raya, A. (2017). CRISPR/Cas9-based engineering of the epigenome. Cell Stem Cell, 21(4), 431-447.

Tang, L., Zeng, Y., Du, H., Gong, M., Peng, J., Zhang, B., ... & Liu, J. (2017). CRISPR/Cas9-mediated gene editing in human zygotes using Cas9 protein. Molecular genetics and genomics, 292(3), 525-533.

Rauch, B. J., Silvis, M. R., Hultquist, J. F., Waters, C. S., McGregor, M. J., Krogan, N. J., & Bondy-Denomy, J. (2017). Inhibition of CRISPR-Cas9 with bacteriophage proteins. Cell, 168(1-2), 150-158.

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For research use only. Not intended for any clinical use.

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