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Rabbit Anti-CRTC3 Recombinant Antibody (C35G4) (CBMAB-T0195-YJ)

Provided herein is a Rabbit monoclonal antibody, which binds to CRTC3 (CREB Regulated Transcription Coactivator 3). The antibody can be used for immunoassay techniques, such as WB.
See all CRTC3 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat, Monkey
Clone
C35G4
Antibody Isotype
IgG
Application
WB

Basic Information

Specificity
Human, Mouse, Rat, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CREB Regulated Transcription Coactivator 3
Introduction
CRTC3 (CREB Regulated Transcription Coactivator 3) is a Protein Coding gene. Diseases related to CRTC3 include Human T-Cell Leukemia Virus Type 1 and Mucoepidermoid Carcinoma. Among its related pathways are Organelle biogenesis and maintenance and Mitochondrial Gene Expression. Gene Ontology (GO) annotations related to CRTC3 include cAMP response element binding protein binding. An important paralog of CRTC3 is CRTC2.
Entrez Gene ID
Human64784
Mouse70461
Rat365297
Monkey709458
UniProt ID
HumanQ6UUV7
MouseQ91X84
RatF1LVL6
MonkeyK7CIH9
Alternative Names
CREB Regulated Transcription Coactivator 3; TORC-3; TORC3; Transducer Of Regulated CAMP Response Element-Binding Protein (CREB) 3; Transducer Of Regulated CAMP Response Element-Binding Protein 3; CREB-Regulated Transcription Coactivator 3; Transducer Of Regulated CREB Protein 3; Transducer Of CREB Protein 3
Function
Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).
Biological Process
Energy homeostasis Source: Ensembl
Macrophage activation Source: CACAO
Negative regulation of cAMP-mediated signaling Source: Ensembl
Negative regulation of lipid catabolic process Source: Ensembl
Positive regulation of CREB transcription factor activity Source: GO_Central
Positive regulation of transcription by RNA polymerase II Source: GO_Central
Protein homotetramerization Source: InterPro
Viral process Source: UniProtKB-KW
Cellular Location
Nucleus; Cytoplasm. Appears to be mainly nuclear (PubMed:15454081). Translocates to the nucleus following adenylyl cyclase or MAP kinase activation (PubMed:30611118).
PTM
Phosphorylation/dephosphorylation states of Ser-273 are required for regulating transduction of CREB activity (By similarity). CRTCs/TORCs are inactive when phosphorylated, and active when dephosphorylated at this site (By similarity). May be phosphorylated at Ser-391 by MAPK3/ERK1 and/or MAPK1/ERK2 or by some cyclin-dependent kinases such as CDK1,CDK2 or CDK5 (PubMed:30611118). Following adenylyl cyclase activation, dephosphorylated at Ser-162 and Ser-273 resulting in its dissociation from 14-3-3 proteins probably promoting CRTC3 translocation into the nucleus (PubMed:30611118).

Smith, L. I., Zhao, Z., Walker, J., Lightman, S., & Spiga, F. (2021). Activation and expression of endogenous CREB‐regulated transcription coactivators (CRTC) 1, 2 and 3 in the rat adrenal gland. Journal of Neuroendocrinology, 33(1), e12920.

Liu, J., Wang, L., Chen, W., Li, J., & Shan, T. (2021). CRTC3 regulates the lipid metabolism and adipogenic differentiation of porcine Intramuscular and subcutaneous adipocytes by activating the calcium pathway. Journal of Agricultural and Food Chemistry, 69(25), 7243-7255.

Kim, J. H., Hong, A. R., Kim, Y. H., Yoo, H., Kang, S. W., Chang, S. E., & Song, Y. (2020). JNK suppresses melanogenesis by interfering with CREB-regulated transcription coactivator 3-dependent MITF expression. Theranostics, 10(9), 4017.

Liu, J., Nong, Q., Wang, J., Chen, W., Xu, Z., You, W., ... & Shan, T. (2020). Breed difference and regulatory role of CRTC3 in porcine intramuscular adipocyte. Animal Genetics, 51(4), 521-530.

Xu, Z., Liu, J., You, W., Wang, Y., & Shan, T. (2019). Cold exposure induces nuclear translocation of CRTC3 in brown adipose tissue. Journal of Cellular Biochemistry, 120(6), 9138-9146.

Sonntag, T., Ostojić, J., Vaughan, J. M., Moresco, J. J., Yoon, Y. S., Yates III, J. R., & Montminy, M. (2019). Mitogenic signals stimulate the CREB coactivator CRTC3 through PP2A recruitment. Iscience, 11, 134-145.

Kim, Y. H., Yoo, H., Hong, A. R., Kwon, M., Kang, S. W., Kim, K., & Song, Y. (2018). NEDD4L limits cAMP signaling through ubiquitination of CREB‐regulated transcription coactivator 3. The FASEB Journal, 32(7), 4053-4062.

Yoon, Y. S., Tsai, W. W., Van de Velde, S., Chen, Z., Lee, K. F., Morgan, D. A., ... & Montminy, M. (2018). cAMP-inducible coactivator CRTC3 attenuates brown adipose tissue thermogenesis. Proceedings of the National Academy of Sciences, 115(23), E5289-E5297.

Kim, J. H., Hedrick, S., Tsai, W. W., Wiater, E., Le Lay, J., Kaestner, K. H., ... & Montminy, M. (2017). CREB coactivators CRTC2 and CRTC3 modulate bone marrow hematopoiesis. Proceedings of the National Academy of Sciences, 114(44), 11739-11744.

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For research use only. Not intended for any clinical use.

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