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Mouse Anti-CUL4A Recombinant Antibody (129) (CBMAB-AP1789LY)


See all CUL4A antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
129
Antibody Isotype
IgG1
Application
ELISA, IF, IHC, WB

Basic Information

Immunogen
Fusion protein of CUL4A
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cullin 4A
Introduction
CUL4A (Cullin 4A) is a Protein Coding gene. Diseases associated with CUL4A include Xeroderma Pigmentosum Group E and Xeroderma Pigmentosum, Variant Type. Among its related pathways are Transcription-Coupled Nucleotide Excision Repair (TC-NER) and Nucleotide excision repair. Gene Ontology (GO) annotations related to this gene include ubiquitin protein ligase binding. An important paralog of this gene is CUL4B.
Entrez Gene ID
Human8451
Mouse99375
Rat361181
UniProt ID
HumanQ13619
MouseQ3TCH7
RatB2RYJ3
Alternative Names
Cullin 4A; CUL-4A; Cullin-4A;
Function
Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. DCX(DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled (H3.1) and replication-independent (H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9. DCX(DTL) directs autoubiquitination of DTL. The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1 (PubMed:26431207).

With CUL4B, contributes to ribosome biogenesis (PubMed:26711351).
Biological Process
Cellular response to DNA damage stimulus Source: GO_Central
DNA damage response, detection of DNA damage Source: Reactome
G1/S transition of mitotic cell cycle Source: ProtInc
Global genome nucleotide-excision repair Source: Reactome
Hemopoiesis Source: Ensembl
Intrinsic apoptotic signaling pathway Source: ProtInc
In utero embryonic development Source: Ensembl
Negative regulation of granulocyte differentiation Source: Ensembl
Nucleotide-excision repair, DNA damage recognition Source: Reactome
Nucleotide-excision repair, DNA duplex unwinding Source: Reactome
Nucleotide-excision repair, DNA incision Source: Reactome
Nucleotide-excision repair, DNA incision, 3'-to lesion Source: Reactome
Nucleotide-excision repair, DNA incision, 5'-to lesion Source: Reactome
Nucleotide-excision repair, preincision complex assembly Source: Reactome
Nucleotide-excision repair, preincision complex stabilization Source: Reactome
Positive regulation of cell population proliferation Source: Ensembl
Positive regulation of G1/S transition of mitotic cell cycle Source: Ensembl
Post-translational protein modification Source: Reactome
Proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
Protein ubiquitination Source: UniProtKB
Regulation of DNA damage checkpoint Source: Ensembl
Regulation of nucleotide-excision repair Source: Ensembl
Regulation of protein metabolic process Source: Ensembl
Rhythmic process Source: UniProtKB-KW
Ribosome biogenesis Source: UniProtKB
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: GO_Central
Somatic stem cell population maintenance Source: Ensembl
Transcription-coupled nucleotide-excision repair Source: Reactome
Viral process Source: UniProtKB-KW
Cellular Location
Nucleoplasm; Cul4-RING E3 ubiquitin ligase complex; Cul4A-RING E3 ubiquitin ligase complex; Cullin-RING ubiquitin ligase complex; SCF ubiquitin ligase complex
PTM
Neddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
(Microbial infection) Deneddylated by Epstein-Barr virus BPLF1 leading to a S-phase-like environment that is required for efficient replication of the viral genome.

Lung, J., Chen, Y. C., Lin, Y. C., Li, Y. C., You, L., Jablons, D. M., ... & Hung, M. S. (2021). The effect of cullin 4A on lung cancer cell chemosensitivity to paclitaxel through p33ING1b regulation. American Journal of Translational Research, 13(10), 11194.

Wang, X., & Chen, T. (2021). CUL4A regulates endometrial cancer cell proliferation, invasion and migration by interacting with CSN6. Molecular Medicine Reports, 23(1), 1-1.

Jin, X., Ma, Y. C., Zhu, W. Y., & Fan, L. (2019). CUL4A expression is associated with tumor stage and prognosis in nasopharyngeal carcinoma. Medicine, 98(51).

Hung, M. S., Chen, Y. C., Lin, P. Y., Li, Y. C., Hsu, C. C., Lung, J. H., ... & Yang, C. T. (2019). Cul4A modulates invasion and metastasis of lung cancer through regulation of ANXA10. Cancers, 11(5), 618.

Chen, G., Zhao, X., Tan, Z., Wang, D., Luo, D., Zhang, P., ... & Li, L. (2018). Investigation of the role of cullin 4A overexpression in human liver cancer. Molecular Medicine Reports, 18(3), 2531-2540.

Li, C., Bu, J., Liao, Y., Zhang, J., Han, J., Zhang, H., ... & Yang, T. (2018). High expressions of CUL4A and TP53 in colorectal cancer predict poor survival. Cellular Physiology and Biochemistry, 51(6), 2829-2842.

Lian, Y., Yan, C., Xu, H., Yang, J., Yu, Y., Zhou, J., ... & Wang, K. (2018). A novel lncRNA, LINC00460, affects cell proliferation and apoptosis by regulating KLF2 and CUL4A expression in colorectal cancer. Molecular Therapy-Nucleic Acids, 12, 684-697.

Yang, Y., Wang, S., Li, J., Qi, S., & Zhang, D. (2017). CUL4A as a marker and potential therapeutic target in multiple myeloma. Tumor Biology, 39(7), 1010428317703923.

Zhang, T. J., Xue, D., Zhang, C. D., Zhang, Z. D., Liu, Q. R., & Wang, J. Q. (2017). Cullin 4A is associated with epithelial to mesenchymal transition and poor prognosis in perihilar cholangiocarcinoma. World journal of gastroenterology, 23(13), 2318.

Gong, Y., Xiang, X. J., Feng, M., Chen, J., Fang, Z. L., & Xiong, J. P. (2017). CUL4A promotes cell invasion in gastric cancer by activating the NF-κB signaling pathway. Biologics: Targets & Therapy, 11, 45.

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For research use only. Not intended for any clinical use.

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