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Mouse Anti-CUL5 Recombinant Antibody (ZC009) (CBMAB-C11339-LY)

The product is antibody recognizes CUL5. The antibody ZC009 immunoassay techniques such as: ELISA, WB.
See all CUL5 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
ZC009
Antibody Isotype
IgG1, κ
Application
ELISA, WB

Basic Information

Immunogen
Synthetic peptide derived from the N-terminal region of the mouse and rat CUL-5/VACM-1 proteins, which are 89% homologous to human
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Preservative
0.1% sodium azide
Concentration
0.5 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cullin 5
Introduction
CUL5 (Cullin 5) is a Protein Coding gene. Among its related pathways are Innate Immune System and CDK-mediated phosphorylation and removal of Cdc6. Gene Ontology (GO) annotations related to this gene include protein heterodimerization activity and ubiquitin protein ligase binding. An important paralog of this gene is CUL1.
Entrez Gene ID
Human8065
Mouse75717
Rat64624
UniProt ID
HumanQ93034
MouseQ9D5V5
RatQ9JJ31
Function
Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAK2. Seems to be involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor.
Biological Process
ERBB2 signaling pathway Source: Reactome
G1/S transition of mitotic cell cycle Source: ProtInc
Intrinsic apoptotic signaling pathway Source: ProtInc
Post-translational protein modification Source: Reactome
Protein ubiquitination Source: GO_Central
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: GO_Central
Viral process Source: UniProtKB-KW
Cellular Location
Cytosol; Cul5-RING ubiquitin ligase complex; Cullin-RING ubiquitin ligase complex; SCF ubiquitin ligase complex; Site of DNA damage
PTM
Neddylated; which enhances the ubiquitination activity of SCF and prevents binding of the inhibitor CAND1. Deneddylated via its interaction with the COP9 signalosome (CSN).

Bano, I., Soomro, A. S., Abbas, S. Q., Ahmadi, A., Hassan, S. S. U., Behl, T., & Bungau, S. (2022). A Comprehensive Review of Biological Roles and Interactions of Cullin-5 Protein. ACS omega.

Xu, S., Ma, Y., Tong, Q., Yang, J., Liu, J., Wang, Y., ... & Zhang, J. (2021). Cullin-5 neddylation-mediated NOXA degradation is enhanced by PRDX1 oligomers in colorectal cancer. Cell death & disease, 12(3), 1-16.

Li, Z., Hu, N., Dai, L., Hou, X., Hu, W., Liang, W., & Wang, X. (2021). Cullin-5 (CUL5) as a potential prognostic marker in a pan-cancer analysis of human tumors. Bioengineered, 12(1), 5348-5360.

Gao, F., Fan, Y., Zhou, B., Guo, W., Jiang, X., Shi, J., & Ren, C. (2020). The functions and properties of cullin-5, a potential therapeutic target for cancers. American Journal of Translational Research, 12(2), 618.

Jia, Y., Ramamoorthi, G., Kodumudi, K., Basu, A., Wiener, D., & Czerniecki, B. (2019). Th1 cytokines promotes E3 ubiquitin ligase Cullin 5 expression via STAT1 signaling cascade and enhance cul5 mediated proteasomal degradation of HER2 in HER2+/ER-breast cancer.

Si, L., Jia, Y., Lin, R., Jian, W., Yu, Q., & Yang, S. (2019). MicroRNA-27a regulates the proliferation, chemosensitivity and invasion of human ovarian cancer cell lines by targeting Cullin 5. Archives of biochemistry and biophysics, 668, 9-15.

Tapia-Laliena, M. Á., Korzeniewski, N., Peña-Llopis, S., Scholl, C., Fröhling, S., Hohenfellner, M., ... & Duensing, S. (2019). Cullin 5 is a novel candidate tumor suppressor in renal cell carcinoma involved in the maintenance of genome stability. Oncogenesis, 8(1), 1-11.

Talamantez-Lyburn, S., Brown, P., Hondrogiannis, N., Ratliff, J., Wicks, S. L., Nana, N., ... & Ehrlich, E. S. (2019). Gold nanoparticles loaded with cullin-5 DNA increase sensitivity to 17-AAG in cullin-5 deficient breast cancer cells. International journal of pharmaceutics, 564, 281-292.

Zhao, G., Gong, L., Su, D., Jin, Y., Guo, C., Yue, M., ... & Ji, H. (2019). Cullin5 deficiency promotes small-cell lung cancer metastasis by stabilizing integrin β1. The Journal of clinical investigation, 129(3), 972-987.

Gu, Q., Zhang, Z., Gertzen, C. G., Häussinger, D., Gohlke, H., & Münk, C. (2018). Identification of a conserved interface of human immunodeficiency virus type 1 and feline immunodeficiency virus Vifs with Cullin 5. Journal of virology, 92(6), e01697-17.

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For research use only. Not intended for any clinical use.

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