Mouse Anti-CUL7 Recombinant Antibody (CBXC-0626) (CBMAB-C3114-CQ)

This product is a mouse antibody that recognizes CUL7. The antibody CBXC-0626 can be used for immunoassay techniques such as: ELISA, WB.
See all CUL7 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBXC-0626

Antibody Isotype

IgG1, κ

Application

ELISA, WB

Basic Information

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

cullin 7

Introduction

CUL7 (Cullin 7) is a Protein Coding gene. Diseases associated with CUL7 include Three M Syndrome 1 and Dubowitz Syndrome. Among its related pathways are Innate Immune System and Class I MHC mediated antigen processing and presentation. Gene Ontology (GO) annotations related to this gene include binding and ubiquitin protein ligase binding. An important paralog of this gene is CUL9.

Entrez Gene ID

9820

UniProt ID

Q14999

Alternative Names

Cullin 7; KIAA0076; CUL-7; Cullin-7; DJ20C7.5; 3M1;

Function

Core component of the 3M and Cul7-RING(FBXW8) complexes, which mediates the ubiquitination of target proteins. Core component of the 3M complex, a complex required to regulate microtubule dynamics and genome integrity. It is unclear how the 3M complex regulates microtubules, it could act by controlling the level of a microtubule stabilizer (PubMed:24793695).

Interaction with CUL9 is required to inhibit CUL9 activity and ubiquitination of BIRC5 (PubMed:24793696).

Core component of a Cul7-RING ubiquitin-protein ligase with FBXW8, which mediates ubiquitination and consequent degradation of target proteins such as GORASP1, IRS1 and MAP4K1/HPK1 (PubMed:21572988, PubMed:24362026).

Ubiquitination of GORASP1 regulates Golgi morphogenesis and dendrite patterning in brain (PubMed:21572988).

Mediates ubiquitination and degradation of IRS1 in a mTOR-dependent manner: the Cul7-RING(FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2) (PubMed:18498745).

The Cul7-RING(FBXW8) complex also mediates ubiquitination of MAP4K1/HPK1: recognizes and binds autophosphorylated MAP4K1/HPK1, leading to its degradation, thereby affecting cell proliferation and differentiation (PubMed:24362026).

Acts as a regulator in trophoblast cell epithelial-mesenchymal transition and placental development (PubMed:20139075).

Does not promote polyubiquitination and proteasomal degradation of p53/TP53 (PubMed:16547496, PubMed:17332328).

While the Cul7-RING(FBXW8) and the 3M complexes are associated and involved in common processes, CUL7 and the Cul7-RING(FBXW8) complex may be have additional functions.

Biological Process

Epithelial to mesenchymal transition Source: UniProtKB
Golgi organization Source: UniProtKB
IRE1-mediated unfolded protein response Source: Reactome
Microtubule cytoskeleton organization Source: UniProtKB
Mitotic cytokinesis Source: UniProtKB
Placenta development Source: UniProtKB
Positive regulation of dendrite morphogenesis Source: UniProtKB
Post-translational protein modification Source: Reactome
Protein ubiquitination Source: UniProtKB
Proteolysis Source: UniProtKB
Regulation of mitotic nuclear division Source: UniProtKB
Ubiquitin-dependent protein catabolic process Source: InterPro
Vasculogenesis Source: UniProtKB
Viral process Source: UniProtKB-KW

Cellular Location

Centrosome; Cytoplasm; Golgi apparatus; Perinuclear region. Colocalizes with FBXW8 at the Golgi apparatus in neurons; localization to Golgi is mediated by OBSL1. During mitosis, localizes to the mitotic apparatus (PubMed:24793695). CCDC8 is required for centrosomal location (PubMed:24793695).

Involvement in disease

3M syndrome 1 (3M1):
An autosomal recessive disorder characterized by severe pre- and postnatal growth retardation, facial dysmorphism, large head circumference, and normal intelligence and endocrine function. Skeletal changes include long slender tubular bones and tall vertebral bodies.

PTM

According to a report, may not be neddylated despite the conserved consensus site for neddylation at Lys-1576.

References

Hu, L., Wang, X., Jin, T., Han, Y., Liu, J., Jiang, M., ... & Huang, S. (2020). Identification of two CUL7 variants in two Chinese families with 3‐M syndrome by whole‐exome sequencing. Journal of Clinical Laboratory Analysis, 34(7), e23265.

Xu, J., Zhang, Z., Qian, M., Wang, S., Qiu, W., Chen, Z., ... & Li, G. (2020). Cullin-7 (CUL7) is overexpressed in glioma cells and promotes tumorigenesis via NF-κB activation. Journal of Experimental & Clinical Cancer Research, 39(1), 1-21.

Shi, L., Du, D., Peng, Y., Liu, J., & Long, J. (2020). The functional analysis of Cullin 7 E3 ubiquitin ligases in cancer. Oncogenesis, 9(10), 1-12.

Kong, Y., Wang, Z., Huang, M., Zhou, Z., Li, Y., Miao, H., ... & Chen, C. (2019). CUL7 promotes cancer cell survival through promoting Caspase‐8 ubiquitination. International Journal of Cancer, 145(5), 1371-1381.

Qiu, N., He, Y., Zhang, S., Hu, X., Chen, M., & Li, H. (2018). Cullin 7 is a predictor of poor prognosis in breast cancer patients and is involved in the proliferation and invasion of breast cancer cells by regulating the cell cycle and microtubule stability. Oncology reports, 39(2), 603-610.

Hsu, P. H., Ma, Y. T., Fang, Y. C., Huang, J. J., Gan, Y. L., Chang, P. T., ... & Jeng, C. J. (2017). Cullin 7 mediates proteasomal and lysosomal degradations of rat Eag1 potassium channels. Scientific reports, 7(1), 1-15.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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