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Rabbit Anti-CYP11A1 Recombinant Antibody (CBWJC-3524) (CBMAB-C4756WJ)

This product is a Rabbit antibody that recognizes CYP11A1. This antibody CBWJC-3524 can be used for immunoassay techniques such as: WB, IP, IF.
See all CYP11A1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
CBWJC-3524
Antibody Isotype
IgG
Application
WB, IP, IF

Basic Information

Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cytochrome P450 Family 11 Subfamily A Member 1
Introduction
CYP11A1 (Cytochrome P450 Family 11 Subfamily A Member 1) is a Protein Coding gene. Diseases associated with CYP11A1 include Adrenal Insufficiency, Congenital, With 46,Xy Sex Reversal, Partial Or Complete and Inherited Isolated Adrenal Insufficiency Due To Partial Cyp11a1 Deficiency. Among its related pathways are Aldosterone synthesis and secretion and Metabolism. Gene Ontology (GO) annotations related to this gene include iron ion binding and oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen. An important paralog of this gene is CYP11B2.
Entrez Gene ID
Human1583
Mouse13070
Rat29680
UniProt ID
HumanP05108
MouseQ9QZ82
RatP14137
Alternative Names
Cytochrome P450 Family 11 Subfamily A Member 1; Cytochrome P450, Subfamily XIA (Cholesterol Side Chain Cleavage); Cytochrome P450 11A1; Cytochrome P450(Scc); EC 1.14.15.6; CYPXIA1; CYP11A; Cytochrome P450, Family 11, Subfamily A, Polypeptide 1; Cholesterol Side-Chain Cleavage Enzyme, Mitochondrial;
Function
A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones (PubMed:21636783).

Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal (PubMed:21636783).

Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin) (PubMed:21636783).
Biological Process
C21-steroid hormone biosynthetic process Source: UniProtKB
Cellular response to peptide hormone stimulus Source: GO_Central
Cholesterol metabolic process Source: UniProtKB
Cortisol metabolic process Source: GO_Central
Glucocorticoid biosynthetic process Source: GO_Central
Sterol metabolic process Source: Reactome
Vitamin D metabolic process Source: UniProtKB
Cellular Location
Mitochondrion inner membrane. Localizes to the matrix side of the mitochondrion inner membrane.
Involvement in disease
Adrenal insufficiency, congenital, with 46,XY sex reversal (AICSR):
A rare disorder that can present as acute adrenal insufficiency in infancy or childhood. ACTH and plasma renin activity are elevated and adrenal steroids are inappropriately low or absent; the 46,XY patients have female external genitalia, sometimes with clitoromegaly. The phenotypic spectrum ranges from prematurity, complete underandrogenization, and severe early-onset adrenal failure to term birth with clitoromegaly and later-onset adrenal failure. Patients with congenital adrenal insufficiency do not manifest the massive adrenal enlargement typical of congenital lipoid adrenal hyperplasia.

Heidarzadehpilehrood, R., Pirhoushiaran, M., Abdollahzadeh, R., Binti Osman, M., Sakinah, M., Nordin, N., & Abdul Hamid, H. (2022). A Review on CYP11A1, CYP17A1, and CYP19A1 Polymorphism Studies: Candidate Susceptibility Genes for Polycystic Ovary Syndrome (PCOS) and Infertility. Genes, 13(2), 302.

Lin, Y. C., & Papadopoulos, V. (2021). Neurosteroidogenic enzymes: CYP11A1 in the central nervous system. Frontiers in neuroendocrinology, 62, 100925.

Slominski, R. M., Raman, C., Elmets, C., Jetten, A. M., Slominski, A. T., & Tuckey, R. C. (2021). The significance of CYP11A1 expression in skin physiology and pathology. Molecular and cellular endocrinology, 530, 111238.

Chaiprasongsuk, A., Janjetovic, Z., Kim, T. K., Tuckey, R. C., Li, W., Raman, C., ... & Slominski, A. T. (2020). CYP11A1-derived vitamin D3 products protect against UVB-induced inflammation and promote keratinocytes differentiation. Free Radical Biology and Medicine, 155, 87-98.

Jones, P., Lucock, M., Chaplin, G., Jablonski, N. G., Veysey, M., Scarlett, C., & Beckett, E. (2020). Distribution of variants in multiple vitamin D-related loci (DHCR7/NADSYN1, GC, CYP2R1, CYP11A1, CYP24A1, VDR, RXRα and RXRγ) vary between European, East-Asian and Sub-Saharan African-ancestry populations. Genes & Nutrition, 15(1), 1-11.

Maharaj, A., Buonocore, F., Meimaridou, E., Ruiz-Babot, G., Guasti, L., Peng, H. M., ... & Metherell, L. A. (2019). Predicted benign and synonymous variants in CYP11A1 cause primary adrenal insufficiency through missplicing. Journal of the Endocrine Society, 3(1), 201-221.

Kolli, V., Kim, H., Torky, A., Lao, Q., Tatsi, C., Mallappa, A., & Merke, D. P. (2019). Characterization of the CYP11A1 nonsynonymous variant p. E314K in children presenting with adrenal insufficiency. The Journal of Clinical Endocrinology & Metabolism, 104(2), 269-276.

Su, H., Wang, B., & Shaik, S. (2019). Quantum-mechanical/molecular-mechanical studies of CYP11A1-catalyzed biosynthesis of pregnenolone from cholesterol reveal a C–C bond cleavage reaction that occurs by a compound i-mediated electron transfer. Journal of the American Chemical Society, 141(51), 20079-20088.

Pan, T., He, G., Chen, M., Bao, C., Chen, Y., Liu, G., ... & Liu, X. (2017). Abnormal CYP11A1 gene expression induces excessive autophagy, contributing to the pathogenesis of preeclampsia. Oncotarget, 8(52), 89824.

Chien, Y., Rosal, K., & Chung, B. C. (2017). Function of CYP11A1 in the mitochondria. Molecular and cellular endocrinology, 441, 55-61.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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