Sign in or Register   Sign in or Register
  |  

Mouse Anti-CYP17A1 Recombinant Antibody (6E1-H9-F6) (CBMAB-C8240-LY)

This product is antibody recognizes CYP17A1. The antibody 6E1-H9-F6 immunoassay techniques such as: WB.
See all CYP17A1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
6E1-H9-F6
Antibody Isotype
IgG2b
Application
WB

Basic Information

Immunogen
Purified recombinant human CYP17A1 protein fragments expressed in E.coli
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cytochrome P450 Family 17 Subfamily A Member 1
Introduction
CYP11B2 (Cytochrome P450 Family 11 Subfamily B Member 2) is a Protein Coding gene. Diseases associated with CYP11B2 include Corticosterone Methyloxidase Type I Deficiency and Corticosterone Methyloxidase Type Ii Deficiency. Among its related pathways are superpathway of steroid hormone biosynthesis and Metabolism. Gene Ontology (GO) annotations related to this gene include iron ion binding and oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen.
An important paralog of this gene is CYP11B1.
Entrez Gene ID
1585
UniProt ID
P19099
Alternative Names
Cytochrome P450 Family 11 Subfamily B Member 2; Cytochrome P450, Subfamily XIB (Steroid 11-Beta-Hydroxylase), Polypeptide 2; Cytochrome P450, Family 11, Subfamily B, Polypeptide 2; Aldosterone-Synthesizing Enzyme; Steroid 11-Beta-Monooxygenase; Aldosterone Synthase; Cytochrome P-450Aldo; Cytochrome P-450C18; EC 1.14.15.4; CYPXIB2; ALDOS; Mitochondrial Cytochrome P450, Family 11, Subfamily B, Polypeptide 2; Steroid 18-Hydroxylase, Aldosterone Synthase, P450C18, P450aldo;
Function
A cytochrome P450 monooxygenase involved in corticoid and androgen biosynthesis (PubMed:9452426, PubMed:27339894, PubMed:22266943, PubMed:25301938).

Catalyzes 17-alpha hydroxylation of C21 steroids, which is common for both pathways. A second oxidative step, required only for androgen synthesis, involves an acyl-carbon cleavage. The 17-alpha hydroxy intermediates, as part of adrenal glucocorticoids biosynthesis pathway, are precursors of cortisol (PubMed:9452426, PubMed:25301938) (Probable).

Hydroxylates steroid hormones, pregnenolone and progesterone to form 17-alpha hydroxy metabolites, followed by the cleavage of the C17-C20 bond to form C19 steroids, dehydroepiandrosterone (DHEA) and androstenedione (PubMed:9452426, PubMed:27339894, PubMed:22266943, PubMed:25301938).

Has 16-alpha hydroxylase activity. Catalyzes 16-alpha hydroxylation of 17-alpha hydroxy pregnenolone, followed by the cleavage of the C17-C20 bond to form 16-alpha-hydroxy DHEA. Also 16-alpha hydroxylates androgens, relevant for estriol synthesis (PubMed:27339894, PubMed:25301938).

Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:9452426, PubMed:27339894, PubMed:22266943, PubMed:25301938).
Biological Process
Androgen biosynthetic process Source: Reactome
Glucocorticoid biosynthetic process Source: Reactome
Hormone biosynthetic process Source: UniProtKB
Progesterone metabolic process Source: UniProtKB
Sex differentiation Source: ProtInc
Steroid biosynthetic process Source: ProtInc
Steroid metabolic process Source: UniProtKB
Cellular Location
Endoplasmic reticulum membrane; Microsome membrane
Involvement in disease
Adrenal hyperplasia 5 (AH5):
A form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: 'salt wasting' (SW, the most severe type), 'simple virilizing' (SV, less severely affected patients), with normal aldosterone biosynthesis, 'non-classic form' or late-onset (NC or LOAH) and 'cryptic' (asymptomatic).
PTM
Phosphorylation is necessary for 17,20-lyase, but not for 17-alpha-hydroxylase activity.

Zhai, G., Shu, T., Chen, K., Lou, Q., Jia, J., Huang, J., ... & Yin, Z. (2022). Successful production of an all-female common carp (Cyprinus carpio L.) population using cyp17a1-deficient neomale carp. Engineering, 8, 181-189.

Liu, Y., Denisov, I. G., Sligar, S. G., & Kincaid, J. R. (2021). Substrate-Specific Allosteric Effects on the Enhancement of CYP17A1 Lyase Efficiency by Cytochrome b 5. Journal of the American Chemical Society, 143(10), 3729-3733.

Tian, J., Li, Y., Fu, H., Ren, L., He, Y., Zhai, S., ... & Liu, S. (2021). Physiological role of CYP17A1-like in cadmium detoxification and its transcriptional regulation in the Pacific oyster, Crassostrea gigas. Science of The Total Environment, 796, 149039.

Xiao, F., Song, X., Tian, P., Gan, M., Verkhivker, G. M., & Hu, G. (2020). Comparative dynamics and functional mechanisms of the CYP17A1 tunnels regulated by ligand binding. Journal of Chemical Information and Modeling, 60(7), 3632-3647.

Lin, H. Y., Ko, C. Y., Kao, T. J., Yang, W. B., Tsai, Y. T., Chuang, J. Y., ... & Hsu, T. I. (2019). CYP17A1 Maintains the survival of glioblastomas by regulating SAR1-mediated endoplasmic reticulum health and redox homeostasis. Cancers, 11(9), 1378.

Kaur, R., Kaur, T., & Kaur, A. (2018). Genetic association study from North India to analyze association of CYP19A1 and CYP17A1 with polycystic ovary syndrome. Journal of assisted reproduction and genetics, 35(6), 1123-1129.

Almassi, N., Reichard, C., Li, J., Russell, C., Perry, J., Ryan, C. J., ... & Sharifi, N. (2018). HSD3B1 and response to a nonsteroidal CYP17A1 inhibitor in castration-resistant prostate cancer. JAMA oncology, 4(4), 554-557.

Zhai, G., Shu, T., Xia, Y., Lu, Y., Shang, G., Jin, X., ... & Yin, Z. (2018). Characterization of sexual trait development in cyp17a1-deficient zebrafish. Endocrinology, 159(10), 3549-3562.

Li, A., Yadav, R., White, J. K., Herroon, M. K., Callahan, B. P., Podgorski, I., ... & Kodanko, J. J. (2017). Illuminating cytochrome P450 binding: Ru (ii)-caged inhibitors of CYP17A1. Chemical Communications, 53(26), 3673-3676.

Sivonova, M. K., Jurecekova, J., Tatarkova, Z., Kaplan, P., Lichardusova, L., & Hatok, J. (2017). The role of CYP17A1 in prostate cancer development: structure, function, mechanism of action, genetic variations and its inhibition. Gen Physiol Biophys, 36(5), 487-499.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-CYP17A1 Recombinant Antibody (6E1-H9-F6)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare