Sign in or Register   Sign in or Register
  |  

Rabbit Anti-CYP21A2 Recombinant Antibody (EG887) (CBMAB-EN1058-LY)

The product is antibody recognizes CYP21A2. The antibody EG887 immunoassay techniques such as: WB: 1:500~1:1000 ELISA: 1:20000.
See all CYP21A2 antibodies

Summary

Host Animal
Rabbit
Specificity
Human
Clone
EG887
Antibody Isotype
IgG
Application
WB: 1:500~1:1000 ELISA: 1:20000

Basic Information

Immunogen
The antibody was produced against synthesized peptide derived from internal of human Cytochrome P450 21A2.
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cytochrome P450 Family 21 Subfamily A Member 2
Introduction
CYP21A2 (Cytochrome P450 Family 21 Subfamily A Member 2) is a Protein Coding gene. Diseases associated with CYP21A2 include Adrenal Hyperplasia, Congenital, Due To 21-Hydroxylase Deficiency and Classic Congenital Adrenal Hyperplasia Due To 21-Hydroxylase Deficiency, Simple Virilizing Form. Among its related pathways are superpathway of steroid hormone biosynthesis and Aldosterone synthesis and secretion. Gene Ontology (GO) annotations related to this gene include iron ion binding and oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen. An important paralog of this gene is CYP17A1.
Entrez Gene ID
1589
UniProt ID
P08686
Alternative Names
Cytochrome P450 Family 21 Subfamily A Member 2; Cytochrome P450, Subfamily XXIA (Steroid 21-Hydroxylase, Congenital Adrenal Hyperplasia), Polypeptide 2; Cytochrome P450, Family 21, Subfamily A, Polypeptide 2; Steroid 21-Monooxygenase; Cytochrome P450-C21B; Cytochrome P450 XXI; 21-OHase; CYP21B; CYP21; Steroid 21 Hydroxylase; Steroid 21-Hydroxylase;
Function
A cytochrome P450 monooxygenase that plays a major role in adrenal steroidogenesis. Catalyzes the hydroxylation at C-21 of progesterone and 17alpha-hydroxyprogesterone to respectively form 11-deoxycorticosterone and 11-deoxycortisol, intermediate metabolites in the biosynthetic pathway of mineralocorticoids and glucocorticoids (PubMed:25855791, PubMed:10602386, PubMed:16984992, PubMed:22014889, PubMed:27721825).

Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:25855791).
Biological Process
Glucocorticoid biosynthetic process Source: GO_Central
Mineralocorticoid biosynthetic process Source: Reactome
Steroid biosynthetic process Source: UniProtKB
Steroid metabolic process Source: UniProtKB
Sterol metabolic process Source: Reactome
Cellular Location
Endoplasmic reticulum membrane; Microsome membrane
Involvement in disease
Adrenal hyperplasia 3 (AH3):
A form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: 'salt wasting' (SW, the most severe type), 'simple virilizing' (SV, less severely affected patients), with normal aldosterone biosynthesis, 'non-classic form' or late-onset (NC or LOAH) and 'cryptic' (asymptomatic).

Gangodkar, P., Khadilkar, V., Raghupathy, P., Kumar, R., Dayal, A. A., Dayal, D., ... & Khadilkar, A. (2021). Clinical application of a novel next generation sequencing assay for CYP21A2 gene in 310 cases of 21-hydroxylase congenital adrenal hyperplasia from India. Endocrine, 71(1), 189-198.

Prado, M. J., Singh, S., Ligabue-Braun, R., Meneghetti, B. V., Rispoli, T., Kopacek, C., ... & Pandey, A. V. (2021). Characterization of Mutations Causing CYP21A2 Deficiency in Brazilian and Portuguese Populations. International journal of molecular sciences, 23(1), 296.

Cohen, M., Pignatti, E., Dines, M., Mory, A., Ekhilevitch, N., Kolodny, R., ... & Tiosano, D. (2020). In silico structural and biochemical functional analysis of a novel CYP21A2 pathogenic variant. International journal of molecular sciences, 21(16), 5857.

Lin, B., Zhang, H., & Zheng, Q. (2020). How do mutations affect the structural characteristics and substrate binding of CYP21A2? An investigation by molecular dynamics simulations. Physical Chemistry Chemical Physics, 22(16), 8870-8877.

Malikova, J., Zingg, T., Fingerhut, R., Sluka, S., Grössl, M., Brixius-Anderko, S., ... & Flück, C. E. (2019). HIV drug efavirenz inhibits CYP21A2 activity with possible clinical implications. Hormone research in paediatrics, 91(4), 262-270.

Santos-Silva, R., Cardoso, R., Lopes, L., Fonseca, M., Espada, F., Sampaio, L., ... & Borges, T. (2019). CYP21A2 gene pathogenic variants: a multicenter study on genotype–phenotype correlation from a Portuguese pediatric cohort. Hormone Research in Paediatrics, 91(1), 33-45.

Concolino, P., & Costella, A. (2018). Congenital adrenal hyperplasia (CAH) due to 21-hydroxylase deficiency: a comprehensive focus on 233 pathogenic variants of CYP21A2 gene. Molecular diagnosis & therapy, 22(3), 261-280.

Simonetti, L., Bruque, C. D., Fernández, C. S., Benavides‐Mori, B., Delea, M., Kolomenski, J. E., ... & Dain, L. (2018). CYP21A2 mutation update: Comprehensive analysis of databases and published genetic variants. Human mutation, 39(1), 5-22.

Khajuria, R., Walia, R., Bhansali, A., & Prasad, R. (2017). The spectrum of CYP21A2 mutations in Congenital Adrenal Hyperplasia in an Indian cohort. Clinica Chimica Acta, 464, 189-194.

Concolino, P., Rizza, R., Costella, A., Carrozza, C., Zuppi, C., & Capoluongo, E. (2017). CYP21A2 intronic variants causing 21-hydroxylase deficiency. Metabolism, 71, 46-51.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Rabbit Anti-CYP21A2 Recombinant Antibody (EG887)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare